LOCUS       sequence-306380-        8934 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway entry clone with PE2 enzyme (with stop codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Bosch JA, Birchak G, Perrimon N
  TITLE     Precise genome engineering in Drosophila using prime editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 
            10.1073/pnas.2021996118.
  PUBMED    33443210
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2021996118. doi"; pages: " 10.1073/pnas.2021996118"
COMMENT     Sequence Label: pEntr_PE2
FEATURES             Location/Qualifiers
     source          1..8934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(280..307)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(399..485)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     534..556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     548..565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     549..565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    581..680
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             737..757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(758..3271,3272..3274,3275..4858)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             758..4858
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7031..7051
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(7071..7170)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7187..7206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7188..7206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7211..7227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7211..7227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7329..7348)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7340..8149
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7407..7426)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8296..8884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8785..8804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
       61 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      121 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      241 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
      301 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
      361 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
      421 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
      481 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
      541 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
      601 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
      661 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgaaacgga cagccgacgg
      721 aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca gcatcggcct
      781 ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
      841 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
      901 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
      961 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     1021 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     1081 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     1141 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     1201 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     1261 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     1321 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     1381 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     1441 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
     1501 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     1561 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     1621 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     1681 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     1741 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     1801 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     1861 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     1921 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     1981 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     2041 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     2101 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     2161 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     2221 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     2281 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     2341 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     2401 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     2461 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     2521 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     2581 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     2641 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     2701 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     2761 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     2821 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     2881 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     2941 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     3001 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     3061 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     3121 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     3181 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     3241 catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct ttctgaagga
     3301 cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa
     3361 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     3421 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     3481 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     3541 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     3601 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     3661 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     3721 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     3781 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     3841 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     3901 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     3961 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     4021 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     4081 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     4141 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     4201 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     4261 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     4321 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     4381 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     4441 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     4501 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     4561 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     4621 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     4681 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     4741 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     4801 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgactc
     4861 tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg agtcagcaac
     4921 accagagagc agtggcggca gcagcggcgg cagcagcacc ctaaatatag aagatgagta
     4981 tcggctacat gagacctcaa aagagccaga tgtttctcta gggtccacat ggctgtctga
     5041 ttttcctcag gcctgggcgg aaaccggggg catgggactg gcagttcgcc aagctcctct
     5101 gatcatacct ctgaaagcaa cctctacccc cgtgtccata aaacaatacc ccatgtcaca
     5161 agaagccaga ctggggatca agccccacat acagagactg ttggaccagg gaatactggt
     5221 accctgccag tccccctgga acacgcccct gctacccgtt aagaaaccag ggactaatga
     5281 ttataggcct gtccaggatc tgagagaagt caacaagcgg gtggaagaca tccaccccac
     5341 cgtgcccaac ccttacaacc tcttgagcgg gctcccaccg tcccaccagt ggtacactgt
     5401 gcttgattta aaggatgcct ttttctgcct gagactccac cccaccagtc agcctctctt
     5461 cgcctttgag tggagagatc cagagatggg aatctcagga caattgacct ggaccagact
     5521 cccacagggt ttcaaaaaca gtcccaccct gtttaatgag gcactgcaca gagacctagc
     5581 agacttccgg atccagcacc cagacttgat cctgctacag tacgtggatg acttactgct
     5641 ggccgccact tctgagctag actgccaaca aggtactcgg gccctgttac aaaccctagg
     5701 gaacctcggg tatcgggcct cggccaagaa agcccaaatt tgccagaaac aggtcaagta
     5761 tctggggtat cttctaaaag agggtcagag atggctgact gaggccagaa aagagactgt
     5821 gatggggcag cctactccga agacccctcg acaactaagg gagttcctag ggaaggcagg
     5881 cttctgtcgc ctcttcatcc ctgggtttgc agaaatggca gcccccctgt accctctcac
     5941 caaaccgggg actctgttta attggggccc agaccaacaa aaggcctatc aagaaatcaa
     6001 gcaagctctt ctaactgccc cagccctggg gttgccagat ttgactaagc cctttgaact
     6061 ctttgtcgac gagaagcagg gctacgccaa aggtgtccta acgcaaaaac tgggaccttg
     6121 gcgtcggccg gtggcctacc tgtccaaaaa gctagaccca gtagcagctg ggtggccccc
     6181 ttgcctacgg atggtagcag ccattgccgt actgacaaag gatgcaggca agctaaccat
     6241 gggacagcca ctagtcattc tggcccccca tgcagtagag gcactagtca aacaaccccc
     6301 cgaccgctgg ctttccaacg cccggatgac tcactatcag gccttgcttt tggacacgga
     6361 ccgggtccag ttcggaccgg tggtagccct gaacccggct acgctgctcc cactgcctga
     6421 ggaagggctg caacacaact gccttgatat cctggccgaa gcccacggaa cccgacccga
     6481 cctaacggac cagccgctcc cagacgccga ccacacctgg tacacggatg gaagcagtct
     6541 cttacaagag ggacagcgta aggcgggagc tgcggtgacc accgagaccg aggtaatctg
     6601 ggctaaagcc ctgccagccg ggacatccgc tcagcgggct gaactgatag cactcaccca
     6661 ggccctaaag atggcagaag gtaagaagct aaatgtttat actgatagcc gttatgcttt
     6721 tgctactgcc catatccatg gagaaatata cagaaggcgt gggtggctca catcagaagg
     6781 caaagagatc aaaaataaag acgagatctt ggccctacta aaagccctct ttctgcccaa
     6841 aagacttagc ataatccatt gtccaggaca tcaaaaggga cacagcgccg aggctagagg
     6901 caaccggatg gctgaccaag cggcccgaaa ggcagccatc acagagactc cagacacctc
     6961 taccctcctc atagaaaatt catcaccctc tggcggctca aaaagaaccg ccgacggcag
     7021 cgaattcgag cccaagaaga agaggaaagt ctaaaagggt gggcgcgccg acccagcttt
     7081 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     7141 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     7201 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     7261 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     7321 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     7381 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     7441 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     7501 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     7561 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     7621 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     7681 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     7741 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     7801 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     7861 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     7921 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     7981 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     8041 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     8101 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     8161 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     8221 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     8281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8461 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttt
//
