LOCUS       sequence-306433-       12168 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway compatible expression plasmid for Drosophila and 
            establishing stable cell lines by Hygromycin selection. Actin5c 
            ubiquitous promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Bosch JA, Birchak G, Perrimon N
  TITLE     Precise genome engineering in Drosophila using prime editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 
            10.1073/pnas.2021996118.
  PUBMED    33443210
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2021996118. doi"; pages: " 10.1073/pnas.2021996118"
COMMENT     Sequence Label: pAct-GW-HygroR
FEATURES             Location/Qualifiers
     source          1..12168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(635..652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(806..1394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(886..905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1565..2356,2357..2425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2188..2207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2426..2530)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2598..2616
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4012..4291
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             4337..5362
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4337..5362
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5725..5790
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5920..5940
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6365..6499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6402..6421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6456..6475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6550..9059
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    9175..9299
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9324..9329,9330..9347,9348..9354)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9408..10067
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9474..9493)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10409..10714
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10593..10612
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10755..10879)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
       61 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt ggaccgctga
      121 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
      181 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
      241 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
      301 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
      361 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
      421 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
      481 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
      541 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
      601 tctggaaacg cggaagtcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1861 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2161 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     2221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2581 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattcacat
     2641 ttgtacgaat ttttttttta tcaaaagttc gagtttttca ccaatttcct catcaaccga
     2701 gcaaggcaaa cggctttgaa taatatggtg ttatatatac atatatcaaa tcgctgctga
     2761 ctgcgtgatt gatggcccca agattacata ttatcgaatc aggattcaga aggagatcaa
     2821 tgtcaaatgc ggacaggaac atgaaagacg cctgttatgc gcaattaaaa atttgggttt
     2881 aattgctgtg gaaactgttg ttggcggcat cttaagttcc tgtttaacaa catcaactac
     2941 ttatgtacgt agaagcgttt aagccatttg catacagatg agaactggct tttgtgctaa
     3001 tcagtcaaga tgactccgat gatgatgact cattacctga ccagttttcg ctgctttctt
     3061 ttcaacaact acttgtatat gtattgtatc caatagcaat acattgaatt tccatggtct
     3121 agtcacgtat tatcatttaa ttgacaccaa gtcgtgttat tgttgagcta tcgagttcag
     3181 ctcaaacatt tcttattccc atgaataagc cggcaaaaat atgcaatcta tgaaagttaa
     3241 tataagcaaa ccttactttg actcaatacc aatgcacttt gtgtcgatag gttcacgcaa
     3301 ttgaggcgat tattccgata acccaagcga ttgactgttc ccgtttcgat tccaattgaa
     3361 atttggaaat gtacaatagt tttgctatat gctgtcaagt acgctcttat cttctctggg
     3421 ttttcttcag agtttcgaaa cgcttcttct tttttttgtt tttttttttt tggaatctcg
     3481 tattttggaa ggggctcccc tctggaattt gttacactgt cgttatcatt gcgaacaagc
     3541 ggcccgaagc tatcagcgac tttaacattt acaatgcact tttttacgac caattaaatg
     3601 tacattttcc tttcttcgcc cgttgataag cgaacgcgat gtggcgcagg caatgtgttg
     3661 ctcttgcgac acaaacgcaa tcaaaatgga ttcaatttcg ctttttccca gtgaaacgaa
     3721 gaacgaaccg accatcatga tatgctcctc tgcatgttgc gtattgaatc aatgacaatt
     3781 tcaattaagc cgcccgttcg tcatgcgttt tcgtgcgctt cgaaatgctg ataacgctgc
     3841 tgtcctccaa ctgctttgca tgtggacaca attccattta tttaattctt ttatttggat
     3901 cggttaaatt aaaaagcgcc ttgttacgca tttaacgttg tttccggtgc gtggtggttt
     3961 catgcttctg ggaacggcaa atgggtttag gattgggaac ccctcatcat ctgttggaat
     4021 atactattca acctacaaaa gtaacgttaa acaacactac tttatatttg atatgaatgg
     4081 ccacaccttt tatgccataa aacatattgt aagagaatac cactcttttt attccttctt
     4141 tccttcttgt acgttttttg ctgtaagtag gtcgtggtgc tggtgttgca gttgaaataa
     4201 cttaaaatat aaatcataaa actcaaacat aaacttgact atttatttat ttattaagaa
     4261 aggaaatata aattataaat tacaacaggt tatggacctg cagccaagct tggcgctcgt
     4321 ccgggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     4381 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     4441 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     4501 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     4561 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     4621 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     4681 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     4741 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     4801 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     4861 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     4921 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     4981 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     5041 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     5101 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     5161 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     5221 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     5281 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     5341 tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg
     5401 cctcagccag caactcgcgc gagcctagca aggcaaatgc gagagaacgg ccttacgctt
     5461 ggtggcacag ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc gggagggccg
     5521 gtcgcagtga ttcaggccct tctggattgt gttggtcccc agggcacgat tgtcatgccc
     5581 acgcactcgg gtgatctgac tgatcccgca gattggagat cgccgcccgt gcctgccgat
     5641 tgggtgcaga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     5701 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     5761 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     5821 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     5881 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     5941 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     6001 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     6061 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     6121 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     6181 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     6241 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     6301 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     6361 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6481 atcttatcat gtctggatcg catgcaattc tatattctaa aaacacaaat gatacttcta
     6541 aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca cctacttctc
     6601 atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc cacaaaccaa
     6661 gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat acataatgaa
     6721 tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag taggagaatg
     6781 cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc caactgacca
     6841 actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc gctccattct
     6901 tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg gcaagataag
     6961 attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt tcgcgatgac
     7021 gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc agttagtgga
     7081 attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt tgactatgac
     7141 aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt ataatggcaa
     7201 tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat ttctgcgtct
     7261 tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt ttcggactgt
     7321 ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga
     7381 tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg
     7441 aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg acgttggagc
     7501 cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt
     7561 tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc tcaacgcacc
     7621 gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt atttaaacaa
     7681 agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact tattgccatg
     7741 tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc tctttcactt
     7801 tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat tacgctgaca
     7861 aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg catttgcaat
     7921 aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca cacaaaaaaa
     7981 aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt tagggtaaca
     8041 acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag agagagagag
     8101 aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct ctctcttcgt
     8161 ctctgttgcg caaacgcata gactgcactg aaaaaatcga ttacctattt tttatgaatg
     8221 aatatttgca ctattactat tcaaaactat taagatagca atcacattca atagccaaat
     8281 actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc tgcatattta
     8341 ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct ggcagctgtt
     8401 tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta gtgaatgccc
     8461 tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa gtgcagatag
     8521 cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat cgctggacat
     8581 tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac aatgagtagt
     8641 tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac cagctgaagt
     8701 atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga cccatggaag
     8761 tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt tgcggaggaa
     8821 attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg gtaaaagttt
     8881 tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc taagcgggct
     8941 ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc ttgtgctgtg
     9001 tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa tccagagacc
     9061 ccggatccag ataccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
     9121 caagtgaatc atctcagtgc aactaaaggg gggatctcga gggatccgat atcaacaagt
     9181 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     9241 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     9301 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     9361 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     9421 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     9481 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     9541 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     9601 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     9661 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     9721 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     9781 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     9841 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     9901 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     9961 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
    10021 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg
    10081 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
    10141 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
    10201 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
    10261 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
    10321 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
    10381 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
    10441 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
    10501 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
    10561 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
    10621 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
    10681 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
    10741 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
    10801 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
    10861 ctttcttgta caaagtggtt gatatcacta gttctagtcg accatgaaga tcaagatcat
    10921 tgccccgcca gagcgcaagt actctgtctg gatcggtggc tccatcctgg cttcgctgtc
    10981 caccttccag cagatgtgga tctccaagca ggagtacgac gagtccggcc cctccattgt
    11041 gcaccgcaag tgcttctaag aaggatcgct tgtctgggca agaggatcag gatcgggatg
    11101 gtcttgattc tgctggagga ggaggaggag aagtcgagga agcagcagcg aaagtgcaag
    11161 tgcgagtggt ggaagtttgg agtgcagcac aacaaaatca acaacaacac caactacaag
    11221 atgaaaagag cggaaccacc tgcacaccat catcactatc atcatcgttt tgggcgcatg
    11281 ttgtgtggtt ccagcgtatt aatataatta atttattcca catgagatat gatatgatat
    11341 actatgtatt ttttgttttt tttttatttg taaaccttta atataacaag aactacaaaa
    11401 aatgaaaatg agcgaaaatg catattctgc cattccacac acacaccaac aacacccaac
    11461 acacgcacac ccacaagctt acacacacac attcgcggca tgacaaggac atcaagataa
    11521 agaagaactt aaagaagata tttcccaaag cgcaaaaaga acacacacac attgcaaaac
    11581 acaaacaaca cactagcgtt ttgtacaatt cgtcagcaac cttatgtatt attttttaat
    11641 tatgatgtaa ttataaacaa agtgaaacaa aaatatgaaa acaaaaagga aaatcaaatc
    11701 tgtcttctct ttctcccgct ctcctcgctc tctgctgcta acctcgccct ctcctctctc
    11761 atctttttgt ctgtctctct tccacatttt tgccggccgg caaaataata acccacacac
    11821 actcacactt ggctgcagtt tcgcgtgcga tattcacaca cattcaagca tacaagcata
    11881 catacatatg tatttttttt ttatttgtac acttttctaa ttgcatgcgt atcgattgat
    11941 aagtttacgc ctgaaaatgt taattaaaat gtgaaaatgc aactgaaaaa ctgatgaaat
    12001 gaaacaacaa caagcgaaca atttgctaca tgtgtattgt ctaacaaccg ttactgcaac
    12061 ggttgcttcg gaaaagggtg aagaggaaga gggcacgagg tcgactagag cggccgccga
    12121 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcg
//
