LOCUS       sequence-306459-       10925 bp    DNA     circular SYN 15-APR-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9B_arr2
FEATURES             Location/Qualifiers
     source          1..10925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2577..2591,2592..2597,2598..2614,2615..2621)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2597..2613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2629..2645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2664..2675
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2682..2708,2709..2711,2712..5198,5199..5201,
                     5202..6788)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     rep_origin      7482..8070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7971..7990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtatatata tcacaatttt gatttaaaag taaattcttt ttttaaaaat acaaaaattt
       61 tttataaatt atatagacca tgatattgta ttaatactaa ttgattttaa tttaaggttg
      121 aactaaaata aacatagttt tgtagaaaat aatttttcat tattttgcta cttgatattg
      181 gatcgtaaaa atattagggc tttacaaagt tcttttaaaa gagaatttag taaagcccta
      241 agtcttttaa taaaaaattt tttgtaaaaa agttggcaaa aatagttttt gctatatatt
      301 tatatataag aaaaaattca atactcaagg agataaaaaa tggaaaactt aaacaataat
      361 ccacaaaaaa ttaatcaaat aaataagcaa actaatttta ttggttttga aaattttata
      421 gaacttgata atcaagttaa tcaaactaaa gaaaaaagta aattaagtaa gataggtaag
      481 aaattacccg gaataagcag tcaagtctag tgcggccgca tggcttgtta tgactgtttt
      541 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
      601 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
      661 ttaaacatca tgaggaggta acgtcatggt aaaagattgg attcctattt ctcatgataa
      721 ttataaacaa gtacaaggac cattttatca tggaactaaa gctaatttag caattggtga
      781 tttactaact acaggattta tttctcattt tgaagatggt cgaattctta aacatattta
      841 tttttcagct ttaatggaac cagcagtttg gggagctgaa cttgctatgt cactatctgg
      901 tcttgaaggt cgtggttata tatatatagt tgaaccaaca ggaccatttg aagatgatcc
      961 aaatcttaca aataaaaaat ttcctggtaa tccaacacaa tcttatagaa cttgtgaacc
     1021 tttaagaatt gttggtgttg ttgaagattg ggaaggacat cctgttgaat taataagagg
     1081 aatgttagat tcattagaag atttaaaacg tcgtggttta catgttattg aagattaacc
     1141 tggcgcgcca aataatgccc gtgtccctgt tgaataggga cacggttttt taacaatttt
     1201 tttattccta ggccttagtg gccggtaccc ggggatcctt attactggcc gctttctagc
     1261 ctggatactt gtctggctag ttgcattaga gagtcagcta gagccctagg gctagctgtt
     1321 tgtgtattcg gagccagtgt agtttttctt tttactaacg atacgggtaa taattgattg
     1381 ccttttacag cctgaccttg agacaattgc aaaagtctgt ccacagaggt ttgacctaat
     1441 aatctaaaat cttgtttgat agtagtaagt ggaggtatgt agcaactaga atcttctgta
     1501 tcatcataac ccacaactga aatatcagcc ccaactctaa gtccactttc agtaatagct
     1561 cgcatagctc ctaaagccat ttgatcatta gcaactaaca tagcagtagg gacaatgcct
     1621 tcatttaaca tttgcattgt ttgctgaaat cctgacatag cagaccaatc tccttctctt
     1681 tctgctattg gttgtatttg atttcttgtt aaatacttgt gccatcctgc taatctcaat
     1741 ctggcactta ctgaacttaa gggacctgca agtagtgcaa tttgttgatg gcctaatgca
     1801 acaaggtgtt caactcctaa ccttgttcca tcttcgtgag aaaaaattat agagtttatt
     1861 ggggtttggt ccgacacatc caaaaacaat gctggaacat tagtacatgc tgcttcaact
     1921 gcaatcgcat cttggtcatc aagaggataa ttaattataa gtccactaac acgttgagct
     1981 aataaattat gtactgctgc cttacaagcc tctacacctg atctctcaac cattgataca
     2041 acaacgcttg caccaagttg atcagctctc gatttaattg cggctacaat ttgactaggc
     2101 gcatgtaatg caagtgatga tgtggcaact ccaattaaaa gtgattgttt ccccgcaagt
     2161 tgctgagcaa cgcgatttgg tatataattc aactctgcca tagctgcttc aaccttctct
     2221 cttgtttttg cagatacatg acttgcttgg ttcacaactc tcgaaaccgt ttggtagcta
     2281 accccggcat attcagcaac atcatacaaa gtaacaggtt tcatggatca ttcctccatg
     2341 ataaaattta aatttctgac tttggcaaat ccttttgcct gttaaaaaga atagcacaaa
     2401 aattaaaaaa aaagaaacgc aaatagcaat agcattaaaa ataattatta ttatttattt
     2461 ctttgattaa aatcaaaagc ctttaatgta ttttcttgat ctttcatgcg cttaacggta
     2521 aaatccaagg tattaagttc accttccact tttctctctc tatcttctct agaaaatcat
     2581 aaaaaattta tttgctttgt gagcggataa caattataat agattcaatt gtgagcggat
     2641 aacaatttca cacagaattc attaaagagg agaaattaca tatggataag aaatactcaa
     2701 taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg
     2761 ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc
     2821 ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt ctcaaacgga
     2881 cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt
     2941 caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg
     3001 tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg
     3061 cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg
     3121 ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc
     3181 attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc
     3241 agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag
     3301 atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg
     3361 ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg
     3421 gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt
     3481 caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg
     3541 ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa
     3601 gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg
     3661 aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt
     3721 ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag
     3781 ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg
     3841 aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca
     3901 acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag
     3961 aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc
     4021 gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc
     4081 ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt
     4141 cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag
     4201 tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg
     4261 tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag
     4321 ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag
     4381 attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat
     4441 ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt
     4501 tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg
     4561 aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg
     4621 tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga
     4681 ttaatggtat tagggataag caatctggca aaacaatatt agattttttg aaatcagatg
     4741 gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag
     4801 acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt
     4861 tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat
     4921 tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa
     4981 atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag
     5041 gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc
     5101 aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag
     5161 aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc
     5221 ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat
     5281 cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc
     5341 taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag
     5401 gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa
     5461 tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg
     5521 ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc
     5581 gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg
     5641 cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg
     5701 agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc
     5761 aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca
     5821 aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg
     5881 gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat
     5941 tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt ccagacaggc ggattctcca
     6001 aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg
     6061 atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg
     6121 ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca
     6181 caattctgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa gctaaaggat
     6241 ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag
     6301 aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg
     6361 ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg
     6421 gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag
     6481 atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt
     6541 tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag
     6601 aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt
     6661 ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc
     6721 ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag
     6781 gtgactaata aaagcttgat cagatcccag cttttttttg aagtgcctgg cagtaagttg
     6841 aaaaaatttg aaataaactt ggactatgtt aataaaaaaa tacaaattaa cgaacaaaaa
     6901 gatttgaatt attatataat ttagttaaaa gcaattttaa atgagggagg tttccatcat
     6961 atggctaaac ctttaagtca agaagaaagt actttaattg aaagagctac tgctactatt
     7021 aatagtattc ctattagtga agattatagt gttgctagtg ctgctttaag tagtgatggt
     7081 agaattttta ctggtgttaa tgtttatcat tttactggtg gtccttgtgc tgaattagtt
     7141 gttttaggta ctgccgccgc tgctgctgct ggtaatctca cttgtattgt tgctattggt
     7201 aatgaaaata gaggtatttt aagtccttgt ggtagatgta gacaagtttt attagattta
     7261 catcctggta ttaaagctat tgttaaagat agtgatggtc aacctactgc tgttggtatt
     7321 agagaattat tacctagtgg ttatgtttgg gaaggttaat gacgtcgccg gagcggtcga
     7381 gttctggacc gaccggctcg ggttctcatg accaaaatcc cttaacgtga gttttcgttc
     7441 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7501 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7561 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7621 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7681 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7741 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7801 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7861 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7921 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7981 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8041 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8101 ctggcctttt gctggccttt tgctcacatg ttctttcctg cactagatcc ccggcaagtt
     8161 catccttagg gttttcatat ctcttaaaaa gtcaatttct gcttgattaa tttcttgtgg
     8221 attattgttt ttgcggtttt tcatttttta tctccttgag tattgaattt tttcttatat
     8281 ataaatatat agcaaaaact atttttgcca acttttttac aaaaaatttt ttattaaaac
     8341 acttagggct ttgctaaatt ctcttttaaa agaacttagt aaagccctat ggatttaaga
     8401 actgattata cttacgtagt aaaaatacta cagatattaa tagtatagac catatcaaaa
     8461 tcataatcaa tttatattta tctaaaattg tagtccatac aaaatacaaa aaaattaggt
     8521 ttttctaaat tctattttaa aagaacttag taacacctaa tctctaaatt acttacaaaa
     8581 ttctaaaatt agatttttag ttcttcatat ttctttaaaa gttttttaag aaaatctttt
     8641 tgattttcaa aaatttcatg catcataaaa ctagtaaatt tggatttttt tttgtaaaaa
     8701 tcataacttt cttgggtttt aagttgaaat cttaatggtt ttattggatt ttgcctagat
     8761 ttttttaacg ccggactttc tttatctttt ataaaattat aagaattttt aataccattt
     8821 tctattaaat aatcttcttt tataagcccc tcttgcagtg cagttgctaa tcttaaatac
     8881 ttatacgctt gagtttttgc tacatcaaag tcttttataa aatcaagaaa agttttataa
     8941 ccatcaaatt tataatattg attatctttt atttctttta aaattttaat attttccacc
     9001 ctgtggaata tttctttttt gaaattgtca tttaactttt cctttagtgt cttgtaataa
     9061 gaaattttac tttcattaat cattaaattt tgttcaacat aagtatctat cttttttgtt
     9121 aaaactttaa caacacgttt tttttccata aaaattttcc tttatttttg caacattttc
     9181 cttaattgat ataattgtta ctattttaga ttttttaata tccacttaag aattaatccc
     9241 tcactagaat attttttatt atagaattca tatacatgtt tttctaaatc tataaattta
     9301 tcaacaattg ctttattata gggtgtatct atcttttctt ttcttaataa tcgtctcata
     9361 ttctttaaat aacaacaaac actgccggtt ttaaacctaa attctatgta atatattttt
     9421 gaaaaagtat atacattttc aaccccgttt atttcatact ttacaaaaac attatttatt
     9481 ggttttctat aaccatagta aatgcctaaa aatttgtccc cgtctttcat aggatataga
     9541 taaagttttt gaatctctga tttatctaat aatttttcaa aaaaaattat acaccgcttt
     9601 tgtttgttac taactactct atgcaatgcc tttaatattt ttgtgtgata tatattttta
     9661 tcaccttttt tttctttcag caggaagata ctttcttttg gaaatttaat tttttgttca
     9721 tttattcttg cagccttaag tccgattttc atatttaatc cttatatagc ctttctattt
     9781 aattctttta aactcaataa acttttttgg tttttcatta gttctaataa ttcgcaataa
     9841 tatgtgcaaa acactttatt gtattctatt ttcttttggt tattcaaata ttcttttata
     9901 attggcttta gaagttcaat atttacttct ttttttagtt gttcaataag gatattgaaa
     9961 atgtttgttt ttaaatcttg ataattttgt tgagaatttt ctttttttct ttcaattgac
    10021 ttctctaatt tacgctttat attgtttaaa tcgctatatt tatgactttc aataataaaa
    10081 tgaggcttaa atttgtaatt ttcgtatatt ttttggaaac ttgtttctaa ttgtttagaa
    10141 ttatattcat ttttttcaaa ttctttttga gtgttgcata gaatttcctt taatttttct
    10201 tgtttttctt tcatacaaga tttagtaaaa tgtatatttt tattttttag taatttaatt
    10261 tctattattt ttatgatttt aagtattttg attatattat ctttatcaat atttaaattg
    10321 aataaaattg taagaaaaat tgaaagaatt tttttacata aaaagttaca tttattgaag
    10381 taatttctta cttgatactt ttctatttca ttaatctttc tttcttcttt tatattattt
    10441 ttattattta aacactcctt taaatctaca ctcccatttt tagtaaatct atctttaagg
    10501 ccccttgaaa ctctattttg aaatcttaaa tctttttttt cttgaaagta tttatagatt
    10561 ttttggtaac attctttttt tgaaaaatta agttgatagt atatttcggt ccccaaattt
    10621 acccctaagt gtttgtgata attagttgtg actttaaatt ctttttctaa tttgtaaagg
    10681 taattttgca gtgtttttag tttaacgggt gtttgactat ttcttttaag gttttcatta
    10741 aagtaataga gtatgttttt ttgtgtgtat ttcttatatg ttttatttat gtacgctagt
    10801 gttgagataa ggactactaa tttatactgt aatttattgt agcaagttgt ggttttactg
    10861 gttttgttta tattagataa gttttccatt tatagctcct tgaataccgg attttttatc
    10921 tataa
//
