LOCUS       sequence-306522-        1834 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018269
FEATURES             Location/Qualifiers
     source          1..1834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1782..1802
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1803..1826
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagaagcg ctggggctac ctgtccctcc ccgtcgtcgt tgcgatcggc gccacctttg
      121 cggcgagctc gaccgtcgcc cagaccgtcg ataccgcccg gatcgagacg ggcggccaga
      181 acgactggct gacgtaccac ggctcctaca agtcctatca ctattctccg ctcagccaga
      241 tcaacacctc gaacgtcggc aacctgtccg tggcctggat ccacatcccg ggccggtcca
      301 cccggggcct gcagagcatg ccgctggcgg ccgacggcgt tctctactat acgggctcgt
      361 actcgcgggt tttcgcgctg aacggcgcca cgggcgaggt catctggagc tacttcccgg
      421 agctggacga ggcgttgatc gccaggcaga cccattcgcc ctacaaccgt ggcgccgccc
      481 ttggcgaggg caaggtctac gtcgggacca tggacggccg ccttatcgcc ctcgacatga
      541 agactgggaa ggtggcctgg gacaccaagc ttatcgactc ccagaagttg accgtgggct
      601 tcaccggcgc cccgctctac gcgaagggca cggtcatcat cggcgcgcag ggcggtgaat
      661 ggcccggacg gggaccgatc ttcgccgtcg acgccgcgac gggcaagaaa aagtgggagt
      721 tcctgacagt cgcgggcacc gacgaggcta tgaagacgtg gggcagtgac tcctggcgca
      781 ccggcggggg cggcggctgg atgccgggca cgtatgactc ggagacgaac accatcctct
      841 ggggcacggc gaacccggcc ccgctgtatg attggagcgg gagcgactat aagacccagg
      901 gggcccgtcc gggcgacaac ctgtatacca cgtcggtgat cgggctggac ctcgactcgg
      961 ggaaactgaa gttctaccac caggagctgc cgcacgatgc ctgggacttc gacagcgcgg
     1021 tgggggagtt cgtcatgctc gaccgcgacg ggcagaagct cgtcgtgcat ccgaacaaga
     1081 gcgggtacat cttcgtctac gaccgcaacc tcggcatcaa gaacgtgtgg cggatcaccc
     1141 aaaacagcaa cttcgtcaag gacatcgacc cgaagaccgg cgagctgatt ggccgccggg
     1201 acttttctgc tggcaaggtg aacgagccgc tgtgcccgca catctcaggt ggagtctcgt
     1261 tcaactctgg ctcgtataac ccgaagaccg gcctctatta caagctcggc caggagtggt
     1321 gcatgacgct ggaggtgatg aagacgaccc ctgtgaccgc tccgcaggcc cagctcaaca
     1381 tcggcgccga tttcaagatt gcgccgccgc ccggcggcga aatttacggc cacctcgacg
     1441 cccgagatcc ggtcaccggc gtgaagaagt gggaggtccg ataccccgaa ccccccctgg
     1501 gctccgtcct cagcacgggt gggaatctcg tcttcgtgcc cgattcgcgc ggcaccctgc
     1561 atgcctacga cgcggagagc ggggccgaac tctggtcgca ctcggatggt acgggacacc
     1621 agggtgggat cgtcagctac gccgtcaacg gtaagcaata cgtcgcggtg accgcgggct
     1681 tcggcggcat ggcgagcgac gactacgccc cgacgttcgg cggcgtctac aagtcgatgc
     1741 cccgcgatga cggcgccctg atcgtctaca gcctgaaggc cgaaaacctc tattttcagg
     1801 gacatcatca ccatcaccat caccactgac agct
//
