LOCUS       sequence-306532-        1879 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018289
FEATURES             Location/Qualifiers
     source          1..1879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1827..1847
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1848..1871
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgacggccac ctccttcaag cgctgcggga tctacgctgc gctctccctc gcccttacgg
      121 ccgcgccagt ctacgcgggc gtgaccgatg aggatatcct cgccgatcaa gagaccacgg
      181 aggacatcgt gacatacggg ctgggcctcc gtgggcagcg ctacagcccg ctcgagagca
      241 tcaaccgcga aacggtccag gaaatccggc ccgtctgggc ctttagcctc ggcggcgaga
      301 agcaacgggg ccaggaatcg cagcccatgg tcaaggatgg ggttatgtat gtcaccgggt
      361 cgtactcccg cgtgtacgcg atcgacgtca agacgggcga cgaactgtgg cagtacgacg
      421 cgcggctccc ggacggcatc atgccgtgct gcgacgtgat caacaggggc gtcgccctgt
      481 atgatgacct cgtgatcttc ggcaccctcg acgccaagct ggtcgcgttg gatgcgaaaa
      541 cgggcaaggt ccggtggaag aagacggtcg ataactacca ggatgggtat agcatcaccg
      601 ccgccccgat cgtggtgaag ggcaacgtca tcacgggtgt gtccggcggc gagttcggcg
      661 tggtgggcaa ggtgcgggcc tatgatgcca agacgggtgc gctcgtgtgg gaacggccta
      721 ccgtggaggg ccatatgggc tacgtgtgga aggacgggaa gaagatggag aacgggatca
      781 gcggcggcaa gccgggccag acctggcccg cggatctgtg gaagagcggc ggcgccgccc
      841 cctggctggg tgggacgtac gacgccgaca ccgacctcct cttcttcggc acgggcaacc
      901 cggcgccgtg gaactcgcac atgcggccgg gggacaacct cttctcctcg tcgcgcctcg
      961 ccatcgaccc cgacgacggc aagatcgttt ggcacttcca gacgaccccc cacgacggct
     1021 gggactttga cggagtgaac gagctcatct cgttcgatta taccgagaac ggcaagccgg
     1081 tcaaggcggc cgcgaccgcg gaccggaacg gcttctttta cgtcctcaac cgtaccaacg
     1141 gcgagttcat ccggggattc ccgttcgtgg acaagatctc atgggcgaag gggctggacg
     1201 acaagggccg cccgatgttc gtctccgaga accgtccggg agatccgacc gagatcaagg
     1261 acggtaagca gggcaacacc gtcacgagcg ccccgtcatt cttgggcggc aagaactgga
     1321 tgcccatggc ctactcggcc aagaccggcc tgttctacgt cccggccaac gagtggcaga
     1381 tggacatctg gaacgagccc gtggcgtaca aaaagggcgc ggcctatctg ggtgccggtt
     1441 tcacgatcaa ggccctcaac gaagattaca tcggcgtcct ccgcgcctat gaccctaaga
     1501 ccggcgagcg ggtctgggag tacaagaact atgcgccgct ctggggcggg gtgctgacca
     1561 ccgccggcgg cctcgtgttt accggcaacc cggaggggta cctcatggcc ttcgatgaca
     1621 agaccggcga gctcttgtac aagttcaaca ccgggtccgg catcgtgggc tcacccgtga
     1681 cgtgggacat ggatggcgaa cagtacatct cggtgctctc tggctggggc ggggccgtcc
     1741 cgctgtgggg gggtgacgtg gccaagcgcg tcaaggactt caaccagggc ggcatggtct
     1801 ggaccttcaa gctcccgaag cgcgccgaaa acctctattt tcagggacat catcaccatc
     1861 accatcacca ctgacagct
//
