LOCUS       sequence-306559-        1945 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018343
FEATURES             Location/Qualifiers
     source          1..1945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1893..1913
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1914..1937
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcgggtcta ccatgcgctc gtcctcgggc tggtccttgc cgggtgcaag aaggtgggcc
      121 cgtcctctga cagcacggcg gcgcgcgtgc agggtggcgc gccgatcgtg gccgacgcgc
      181 gcatcggcac gcctaacccc ccgggcggcg aaggggagtg ggtcatgccc gcgcgcgact
      241 acgcgagcag ccgctattcc accctcgcgc agatcacgtc ggccaatgtc aagaacctca
      301 aggccgcttg gacgttctcc acaggcgtgc tccgcggcca tgagggacag ccgctggtgg
      361 tcaacaaaac catgtatctc gtgaccccgt accccaacgt cgcctatgcc atcgacctca
      421 cgcagcccgg ctacccgctg aagtggaagt tccgccccga gaacgcacag gccgccgccg
      481 gtacggcctg ctgcgacgtg gtcaaccggg gcgccgcgta cgccgatgga aagatcgtgt
      541 ataacctcct ggatggccac acggtcgcgg tggacgccga gtccgggaag cagctgtggc
      601 gcgtccagat ggccgacctc aaccgcgggg agacgatcac gatggcgccc atcgtcgtca
      661 agaaccgggt gatcgtcggc agctcgggag gggagatggg cgtgcgaggg tggatcgcgg
      721 cgctcgatct ggcgacgggc agggagctgt ggcgggcgta caacaccggc tcggacgcgg
      781 acgtcaagat cggcgcgcgg ttcaagccct tctatgccac cgagcgcggc aaggacctcg
      841 gccttacgtc ctggccgggc gactcgtgga aggtcggcgg cggcgccgtc tggggctggc
      901 tgagctacga cccgaccctg aacctggtct accatggcac ctcgaacccg ggcccctgga
      961 accaggacgt ccggccgggc gacaacaagt ggacctgcgc gatcttggcg cgggacgccg
     1021 acaccggcga gctggtctgg gccttccagg tcacgcctca cgacctgtgg gattacgacg
     1081 cagtgaacga gaacatcctc gtggacatgc cggtcaaggg acagccccgc aaggccctcg
     1141 tgcactttga tcgcaacggg ttcgcgtaca cccttgaccg ccagacgggc gaggtcctgc
     1201 tggcccagcc gtacgtcccc atgaactggg ccaagtacat taacctgcag accggccgac
     1261 ccgagatcga ctcgtccaag gccaccaagc aggggaagct cgtcaagaac atctgcccgt
     1321 cgctggaggg cggaaagaac caacagcccg cggccttctc gccccagacc ggcctgttct
     1381 acgtcccgac gaacaatctg tgcatggact tcgagtcccg gccagtcagc tacattgccg
     1441 ggaccccgta catcggcgcg aacgccccgg agaccggcgg accgggtgac tataagggcg
     1501 agttcatggc ctgggacgcc gccaatggcc ggaaggtgtg gggcatccgg gagcccttcc
     1561 ccgtctgggg cggggcgctg gtcaccgccg gcaacgtggt cttctatggc acgctggatg
     1621 gctggtttaa ggcggccaac gcccgtaccg gcgaggtgct gtggcagttc aaggtcgggt
     1681 cgggcgtggt cggcaacccg atcacgtaca ccgcccccga cggcaagcag tatatcgcgg
     1741 tctattcggg catcgggggc gatatggggc tcctgatcgc aggcgacgtg gcggcgaacc
     1801 tcccgttcga tgtgcgcgag cgtggtacca ccctccccga cctggccaag tacacctcct
     1861 ggggcggcat gctgttcgtc ttcgcgctcg ccgaaaacct ctattttcag ggacatcatc
     1921 accatcacca tcaccactga cagct
//
