LOCUS       sequence-306560-        1951 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018345
FEATURES             Location/Qualifiers
     source          1..1951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1899..1919
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1920..1943
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgcggccgtc ggtggcgatc gccgcgatgc tcatctggtg ctgtgccccc gcgctcgccc
      121 aacaggaggg ggcagccctg tatgcgtccc actgcgccca gtgccatgac gggggtgacg
      181 cccagagccg ggtccccgga cgctccgcca tgcagggcat gtccttcgac cacgtgctcg
      241 gcaccctcac gaccggctcg atggcggcga tggccaagga acggtccgat gaggagcgcc
      301 gcgcgatcgc ctcgttcgtg acgggcaaga cagccacggg gggcgtggcg gcggatgcgg
      361 agggccggtg cacccaacag gtgcggggct tcccggagcc cctcgacggc cctcgctgga
      421 acgggtgggg cgtggatgcg ggcaactcgc gcttccaacc ggccgatatg gcgggcttga
      481 ccgccgagga ggtgccccgc ctccggctca agtgggccta cgcgttcccg ggggcctcgg
      541 tgtcattcgc gcctcccacc atcatgggcg ggctcctgtt catcgggggg accgaccgga
      601 aggtgcacgc gctggacgcc cgctcgggct gcacgctctg gacgttcgcg acggatgcgg
      661 cggtccgcgc cgccatctcc ttcgccccgc tccccggcac tgatcagttc gtgatcttct
      721 tcggggatct cagggccaac gcgtacgcag tgaacgcgct cacgggggcc ctcatctgga
      781 agacgaaggt cgaggagcac ccggcggcgc gcatcacggg tgccccgacc ctgcactcgg
      841 gcgtgctgta tgtgcccgtg tcgtcgctgg aggaggccgc gggctcgcag gcgtcgtacg
      901 agtgctgcac cttccgcggc tcggtcgtgg ccctggaggc cgtcaccggt aagccggtgt
      961 ggcaggcgta caccatcccg gaagtcccgc accccaccgg caagaacacg aagggcaccc
     1021 agctcttcgg gccgtcgggc gcggccgtgt ggtcggcgcc gaccgtcgac ccgaagcgga
     1081 acgccgtgta tgtggcgacc tcgaattcct actcgaaccc gccggcggcc acggcggacg
     1141 ccattctggc cttcgagctc gccaccggca agctcctgtg gaagcagcag gccaccccga
     1201 aagatgcctt catcgtcgcc tgctatggcg ccgaccggac aaactgcccg gacgagcacg
     1261 ggcccgacca cgacttcggc caatccccca tcctcgtgac cctgcgtgat ggccggcggg
     1321 tgctggccat cgcccagaag tcgggcgtcg tccatgccct ggatccggac gacgggggga
     1381 agatcctgtg gcagacccgc atcggcaagg gcggtgcgct gggcggctcc gaatggggga
     1441 gcgccgcgga ccaggaccgc atctacgtgg ccaactcgga cgtccgattt ctgcatgacg
     1501 gcacgcggcg cctggactcc acccagggcg gcggactctt cggcctcgat ctggcctcgg
     1561 gcaagatcgc gatggaggtg ccgccggtgg cctgcggcga ccggctgcag tgcagccccg
     1621 ccctctccgc ggccgtctcg ctgattccgg gcgtggtctt ctccggaggc gtgtccgggt
     1681 tcctgcgcgc ctatgccacc gccgacggca agctcctctg ggagttcgac acggcccggg
     1741 attacaccgc cgtcaatggc gtccccgcgc acggcggtgc catcgatggc ccgggcccgg
     1801 tgatcgccgg cggtatgctg tacaccaaca gcggctacgg ccagtggagt ggcgtggcgg
     1861 gcaacgtgct cctcgcgttc gaggtgggca aggaggccga aaacctctat tttcagggac
     1921 atcatcacca tcaccatcac cactgacagc t
//
