LOCUS       sequence-306566-        1969 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018357
FEATURES             Location/Qualifiers
     source          1..1969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1917..1937
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1938..1961
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagaagcc gctcaagtcc tggcttctgg cctcctccgt ggcggcgctg ctcgccgcgc
      121 cggcggtctc gacggccaat tccgatgttg agaagctcac ccaaaatccc gcgaactggg
      181 ccacctgggg cggcgattac gcgggcacgc ggtattcgaa gctgtcggag atcaacgcga
      241 caaacgtgaa gaatctccag ccggcgtggt cgttcagcac cggcgtcctg cggggccacg
      301 aggggggccc cctggtggtc aacgacgtgc tctatatcca cacgccgttc ccgaacaccg
      361 tctacgcaat cgaccagaag acccagagcg tcatctggga gttcacgccc acccaggacg
      421 ccgacgtgac gattcccgtc atgtgctgcg acaccgtcaa ccgcggcctc gcctacgggg
      481 acggcaagat cttcttgcag cagagcgata ccgtcctgac cgccctggac gccaagaccg
      541 gcaagcgcgt gtggtccgtc cagaacggcg atcccaagct cggcatgacg aataccgcgg
      601 ccccgctggt ggttaaggac aaggtcctga ccggcattag cggcggcgag ttcggcgtgc
      661 ggggcttcct tgcggcgtac gatatcaaca ccggcgagct cgcctggaag ggctactcca
      721 tgggcccgga caaagatacc ctcatcgacg cgaagaagac gacgacgtgg aaggacggga
      781 aggtgacccc tgtcggcgag aactcctcgc tctccacgtg ggagggcgac cagtggaaga
      841 tcggaggcgg caccacctgg ggatggacct cgtacgaccc gaagctgaac ctgatctact
      901 atggctcggg gaacccgagc acctggaacc cggtccagcg cccgggcgac aacaagtggt
      961 caatgtccct gtgggcgcgg gacgccgata ccggggcggt caagtgggtc taccagatga
     1021 cgccgcacga cgagtgggac ttcgatggca tcaacgagac ggtcctcgtg gaccaggacg
     1081 tgaagggcaa gccgcataag acgatcgtcc atttcgaccg caatggattc ggctacaccc
     1141 tggaccgcga gactggggag ctgctcgtcg cggagaagtt cgataagtcg gtcaactggg
     1201 ccacccatgt cgacatgaac acgggccgtc cgcaggtggt cgccaagtac agcacgcagc
     1261 aaaacggaga ggatgtgaac acgaagggcg tctgcccggc ggcgctcggg agcaagaacc
     1321 aacagcctgt cagctactcg ccgcagaccg gcctcttcta catctcgggc aatcacctct
     1381 gcatggatta cgagccgttc gaggtctcgt acacggctgg ccagccctac gtcggcgcga
     1441 cgctgacgat gatgccggcc ggggcagacg tgctcacggg gaagcccgac aactcgacca
     1501 accttggcca gttcaccgcg tatgacgcga agaccggcaa gatcgcgtgg tcgaacaagg
     1561 agcagttctc ggtgtggtcc ggctccgtgg cgaccgccgg cggggtggtg ttctatggca
     1621 ccctcgaagg ctacctcaag gccgtcgacg ccaagacggg caaggagctg tataagttca
     1681 agaccccgtc gggcatcatc ggaaacgtga acacctggga attcgggggc aagcagtacg
     1741 tgggcgtcct gagcggcatc ggcggctggg cgggtatcgg catcgccgcg ggcctggatt
     1801 cgggcgagga gggctccaac tcggagggcc tgggagccgt cggcgcctac cgctcgctgt
     1861 cctccttcac caagcttggc ggcactctca ccgtcttcgc gctgccgtcg aatgccgaaa
     1921 acctctattt tcagggacat catcaccatc accatcacca ctgacagct
//
