LOCUS       sequence-306813-       11365 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Yeast expression of Sad1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Jiang Z, Jin X, Li Y, Liu S, Liu XM, Wang YY, Zhao P, Cai X, Liu Y, 
            Tang Y, Sun X, Liu Y, Hu Y, Li M, Cai G, Qi X, Chen S, Du LL, He W
  TITLE     Genetically encoded tags for direct synthesis of EM-visible gold 
            nanoparticles in cells.
  JOURNAL   Nat Methods. 2020 Sep;17(9):937-946. doi: 10.1038/s41592-020-0911-z.
            Epub 2020 Aug 10.
  PUBMED    32778831
REFERENCE   2  (bases 1 to 11365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-020-0911-z"; journalName: "Nat Methods"; date: 
            "2020-09"; volume: "17"; issue: "9"; pages: "937-946"
COMMENT     Sequence Label: pDUAL-P41nmt1-Sad1-GFP
FEATURES             Location/Qualifiers
     source          1..11365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3175..3888
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      3915..4102
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3970..3994)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5766..6560)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      7169..7952
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(8675..8692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8675..8691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8684..8706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8904..9359
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8991..9010)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9201..9222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9376..9395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9495..9517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9555..9573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9641..9745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9746..9814,9815..10606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9964..9983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10777..11365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11266..11285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
      421 gcatgcctgc aggtcgatcg actctagagg atcagaaaat tatcgccata aaagacagaa
      481 taagtcatca gcggttgttt catttcctat attttttttt tattttttta ttttttaata
      541 agggaaaatt taacgtctaa ggatacagaa gattgttagc acattaaagt aataaaggct
      601 taagtagtaa gtgccttagc atgttattgt atttcaaagg acataatcta aaataataac
      661 aatatcattt ctcacaagtt attcaatttt cttttttttt tctaataata tcaagaatgt
      721 attatttgtt tgacataagt caactaattt atttaatatg ctggattaat cttgcagaca
      781 tgtaaattaa caagttttag tcaaataacg ttgaagtttc aatgaactca aataatttct
      841 cttttttttt atataaccat aatctgattt atattttccg cagggatcaa ctgaagttat
      901 gacatttgga ttggatcact tataaccttg gtcgccaaat aatacaaaaa tcagcgttat
      961 aaaacaaaga aggtttttgt taagaaatta atcctctttc ttgataagaa agttgaaccg
     1021 aaattgcaga tactgatata tgaaaataat acccacaatt ttgggaatag cgcaagcctc
     1081 aatttaaaca ataggtgagg acacatgata atgacctcaa tgattgttag aagaaaagag
     1141 cctcattaca aaatcgaaaa atgaatggtt gggtacaagt ttccaaaaca tggtaaagtg
     1201 gactttgcgt atgagacgta aatagaaaaa aacacttgtt atatgttttc tagaattatt
     1261 gttgtctctt tatggttgga tgatgcaaaa tagtaatttc ggttagttgc tgtaaaacac
     1321 cacgagacaa atagatatgg atatttatta aatcaggaaa aacgtaactc tcggctactg
     1381 gatggttcag tcacccaacg attactgggg agagaaaaca gggcaaaagc aaagcttaaa
     1441 ggaatccgat tgtcattcgg caatgtgcag cgaaactaaa aaccggataa tggacctgtt
     1501 aatcgaaaca ttgaagataa aggaagagga atcctggcat atcatcaatt gaataagttg
     1561 aattaattat ttcaatctca ttctcacttt ctgacttata gtcgctttgt taaagctagc
     1621 ggatccatgt ttactaatac accggttgga gggaaaagag agcgtcaaaa tggcgctcat
     1681 ccagcttggt cgacgttagg agcgaactct gctcagattc atcaaaatac ggctgatttg
     1741 gcctccaaaa tgcataaact tcgctataca aagataaggt cccctcctac cagggtgtcc
     1801 attgaatcaa ttactcctaa acaaagattt ccagctccca atttcgaaca ggcttaccac
     1861 tctaatattc gatacgagca agaggaatct gataacgaag aatttgaaaa cgtagtgaaa
     1921 aatggacacg aagcttcgac gaatgtattc tatgaatcag atggagatga tgaagagttt
     1981 gtcaatgaag aatacgaaaa cagcatcgac gaggaaagtg atgatgaagg gtatagcttg
     2041 aacgaggata ccaccgcaac aaatgccagt tttaggtacc cgatgaatca aagatctaca
     2101 aggaaatctc aattttattc ctcaaaattt aaaccattac tttggttcgg tatcactctc
     2161 ttttcgactc tcctaataat tactctactt cacaagggtc aagaatttta ctcaagatca
     2221 ttttcatcag ataattccca accatctaat tcgcccgtac caaacattcc tcctgcatcc
     2281 aatgacacca aaacaagttt aaagccagat attattaaag acttcacaga ttcaccttct
     2341 aaagttggcg gcaatgagga attcgattat tcgacgggag atttaattac aaaaaaggaa
     2401 tttgacaaga ttttacaaca aaaagtagag caattaaaac agtcgctaaa ggaggagatg
     2461 tctaattaca aatcatcggt cccatttgaa gttgaactaa atgacgattg gaagttcttc
     2521 atcgaatcta cggttcgaaa atacttgacc gatccggttt ccatgccgaa ttttgcattg
     2581 ctctcaacag gagcggaagt actacctgcc ttaacctcaa aacgctatgt gagacgacca
     2641 tctgcattca taccacgatt tacctcctat ttctttgatt cgctcgttgt tagaggacac
     2701 gaacctagca tagcattaac tcccaacaac gcagttgcga tgtgttggag ctttcaaggt
     2761 tcagagggac aattaggaat ttccctatca cgccctgtat atgtaaccaa tgtcacaata
     2821 gagcacgttc agcacaagat tgctcatgat ctttctagcg ctcccaagga ctttgagttg
     2881 tgggttcagg gaatgtccag caagatgttt gtattgttgg gaaaggcgag atattcttta
     2941 acggaagact ctattcaaac tttttcgttt gaatcctcca attatattgt cgctgaaccg
     3001 atacagaatg tgatcttaaa aataaaaagc aactggggaa atcccaatta cacatgcttg
     3061 tatcaagttc gagttcatgg gactgtaccc aatgcagacg aacaaccaat accttcttta
     3121 ggtgagaaag ccgaaagtac tgcagagaat acgggtcaag attcatctgg atccatgtct
     3181 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
     3241 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
     3301 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     3361 actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa acaacatgac
     3421 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     3481 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     3541 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     3601 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
     3661 gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat
     3721 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     3781 actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     3841 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaaag atctggcgcg
     3901 ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
     3961 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat
     4021 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc
     4081 ttattgacca cacctctacc ggcagatccg ctagggataa cagggtaata tactagtaag
     4141 atgattcttt tatcgtcgat aacgatgagg atgtagacga ttatgatgag agtgattagc
     4201 ttctaaaatt tggtgttttc aaacgctatt tacagtacaa tctgaaaaaa ttggatgaac
     4261 attgtaaatg gtaggatacg agttccaatt aatgtatttt ttaagtatta cacttagctt
     4321 attaatttta tatatacatt ctacctctgt gggtttaaag acaactcgta cacatactca
     4381 aattatttat gaacattata atgcatattc gtactgtcta ttaaacattt ttttgacatt
     4441 aaaaatttcg tttactaacg tagaaagcgc tgataattga ggctactatt ttaatatcta
     4501 gaaacaaaga ttcattaatt aattaagtaa gctccaggat acttgtatat ttcgttaaaa
     4561 aagcgcaatt tcaacaattc ctatgaacat ccatgatttc atcacaaaat caaacatatc
     4621 aggatttaac gtgaattatt tgctttcttg tgattttcat ctgatttctg gtcatttacg
     4681 ttactgtaaa caagcttttt aaaaaaagga ttttttacgc aatattatcg atatcccaat
     4741 ctgtagtata aaaagtgcaa cagtgttgag tttcccgaaa ccaggcattt ttgcggtagg
     4801 gtctgaaact ttcacgaaac catttcacaa caaaacttct tcaacaacat gtgtgcaaag
     4861 aaaatcgtcg tcttaccagg agaccatatt ggccctgaaa ttgttgcttc tgccttggag
     4921 gttttgaaag tcgttgagaa gaagcgacct gagttaaaac tcgagtttga agaacacaag
     4981 attggaggtg cctctattga tgcctatgga acccctttga ctgacgagac tgtgaaggct
     5041 tgtttggaag ctgacggtgt tcttttgggt gccgttggtg gtcctgaatg gaccaacccc
     5101 aattgtcgtc ctgagcaagg tttattgaag cttcgtaaga gtatgggtgt ttgggccaac
     5161 cttcgacctt gcaactttgc cagcaagtct ttagtcaagt acagcccttt gaagcctgaa
     5221 atcgttgaag gtgtcgattt ttgtgttgta cgagaactta ctggaggttg ttactttggt
     5281 gagcgcactg aggacaacgg atcgggttat gctatggaca cttggcctta cagtttggaa
     5341 gaagtttctc gtattgctcg tttggcggcc gcgggcccaa caccaatgtt tataaccaag
     5401 ttttatcttg tttgtctaca tggtatttta cattcatcta catacatctt tcattggctt
     5461 tgtacatagt tatcattaca agtctaaaaa aattcactct tttcttattc aatgtcaatc
     5521 caagagaaaa gattgtggta atgttgtagg agcatgttta ataaattact atagcaaatt
     5581 actttttatt cccaaggtgt ttatctataa tagttaatat tttagtcgct acataaaatt
     5641 ttaccaaaga gtacttgtat actaattcta aatgccttct gacataaaac gcctaggaaa
     5701 acaaacgcaa acaaggcatc gactttttca ataaccaacc aaaaaaattt tacattagtc
     5761 tttttttaat gctgagaaag tctttgctga tatgccttcc aaccagcttc tctatatctc
     5821 ttggcttcga caacaggatt acgaccagct ccatagactc cacgaccaac aatgatgatg
     5881 tcgctaccgc agtttacaat cacttcttca ggagtacgat attgctgtcc cagcccgtct
     5941 cctttaacat ccaagccgat accaggggac atagttatgt agtcgctttg aaggttagga
     6001 aatcgacgac cagctataaa gccaaagcaa aaatcggtat gcttctcaaa ccattctaag
     6061 gttttctctg tgtaggaacc agtagccaaa gagcctttgg aagacatttc agccaaaagc
     6121 aagagaccac gtcccaaagg taaaccaact tctttgaggc cttgtataat accctcgcct
     6181 ggcactgtat ggcaatttgt gatatgagcc caagaagcaa ttttgtacac accagatgca
     6241 tattgtagct tgacggtatt tccaatgtct gcgaatttgc gatcctcaaa gataagaaaa
     6301 cgatgctttt tacctaaggc caccagtttt tctaccatat cctggtcgaa atcctcgaca
     6361 acgtcaatat gtgtcttgat aacacagaca tagggtccaa ttttatctac caattctaag
     6421 atttcggatt tcttcgtcaa atcgaccgcg actgacaagt tgctttgctt ttcttccatc
     6481 aaagccaaca attccttggc aatgggattt ttcatcccct cagctctagc tgaatagctt
     6541 tgaaatactc tagcatccat aactttgctt ttaaaccttt aatttcgatc caagcaaaaa
     6601 agaggttctt ggtaggacaa tacggtaaga aaacacgaca tgtgcagaga tgccgacgaa
     6661 gcatagttaa actgggatgg taaaatcaat taagaattta taaagacaaa attgtataag
     6721 tctctaaaac atcttaatta tacctcacag aactatctaa aatatattca caaagtgcaa
     6781 acattatcat gaaaaagaac cattttaatt taaagcaagg gcattaaggc ttatttacag
     6841 aatttcttac ttttgtaaag attataaggc tgattatctt tttcaccatg ccaaaaatta
     6901 cacaagatag aatggatgtt tgaaattaaa cgtgagtata caaacaaata cactaggtaa
     6961 atcgaaacat ttttttctcc attaagtaac aaattcctat ttagagaaag aatgctgagt
     7021 agattaaata atctatacaa acttttttaa cacaaatgca tacatatagc cagtgggatt
     7081 tgtagctaag cttgcgaatt cgagtctaac tccttaacca ctcggacata gtgacttatc
     7141 tgacactcat tgtaaattaa tatatatata ggcattttgt ttagttaaag gtacttaagt
     7201 aattagtata aacgaaccaa ttttataatc aggaagttaa gtgaatggta gcacatgtcg
     7261 taaaaattgt gaatttttat tgaataatat tttaaataca agcctttcta gactaggtat
     7321 actcataaac atatatgagc aaaaggatag aggagattac attgcatctt ctacaaattt
     7381 atttattgcc ctttactgaa aaattaaata atgagtacta aatgataaaa agcgctcagt
     7441 acaagaaaga ttgcaaaaat attgcattct tcatgaatta aagttgcata taaggcatat
     7501 tgaaagtaat agtactaaaa cagcagttag cgaaaattaa tagaattata ttcgcaagac
     7561 aattgtgaca ttaaattaaa aaattgtaga atttttacta tcctctttaa cgccatgagc
     7621 ctttataaaa aggttaaatt agttttaaca ttcttttttt gagtaagagt ttacaattta
     7681 tcaaaacctg tgttattata ttcattagtt tcaatttatt agcatctaga gaaaaatcaa
     7741 ttggcagtta cattgttgga atttatgaag aaaaagaatc tacaacggag aataagttgc
     7801 tgatcgcttt ccctaaaatt gtatattttg ctgagcttat tttgacattt cgttgaagtt
     7861 ttctctaatt cgcattcatt ttaagtaaaa caatgagaaa taaaattaca aaaaatacaa
     7921 attaaaatac aatttttagc tataattata gacgatgccc ttgtatccca ttctgtctcg
     7981 cttgccccta ctttttatct tttatatacc ataatgaacg ctgccgctac taaccatacc
     8041 ccgattttac atttcggact cccaaggacg tacaaaatag aaaactatag aaaaaaataa
     8101 tcagaaaata gcatgtcatc tctttgtaaa acgcgtttgc aagaagaaag gaaacaatgg
     8161 agaagagatc atccatttgt atgtaaattt tagtaaactt gaagaaatca ctaacaactt
     8221 ctcttactta gggattctat gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa
     8281 tgaattggaa ggttgggccc gcggccgctt agctgaaact tccaaccctc ctgctcccgt
     8341 cacattactc gacaaagcta atgttttggc aacttctcgt ctttggcgca agactgtagc
     8401 taagattttt aaggaggaat atcctcacct taccttaaaa aaccagctca ttgactccgc
     8461 agccatgctt ttggtcaaga gccctcgtac acttaacggt gttgttttga ctgacaactt
     8521 gtttggtgac attatttcag atgaggcttc tgtcattcct ggtagcttgg gccttttgcc
     8581 ttctgcctcc ctttccggtg tggtaggaaa atcagaagaa aaggttcatt gtttggttga
     8641 gcccattcac ggtagcgctc ccgagctcga attcactggc cgtcgtttta caacgtcgtg
     8701 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8761 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8821 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8881 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8941 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     9001 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9061 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     9121 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9181 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     9241 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     9301 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     9361 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     9421 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     9481 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     9541 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     9601 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9661 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9721 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9781 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9841 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9901 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9961 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10021 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10081 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10141 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10201 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10261 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10321 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10381 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10441 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10501 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10561 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10621 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10681 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10921 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11341 gtcagggggg cggagcctat ggaaa
//
