LOCUS       sequence-306868-       10295 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Bioluminescent repair reporter (BLRR) for DNA double strand break 
            (DSB) repairs: homology-directed repair (HDR) and non-homologous end
            joining (NHEJ)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Chien JC, Tabet E, Pinkham K, da Hora CC, Chang JC, Lin S, Badr CE, 
            Lai CP
  TITLE     A multiplexed bioluminescent reporter for sensitive and non-invasive
            tracking of DNA double strand break repair dynamics in vitro and in 
            vivo.
  JOURNAL   Nucleic Acids Res. 2020 Aug 14. pii: 5892751. doi: 
            10.1093/nar/gkaa669.
  PUBMED    32797168
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 14. pii: 5892751. doi: 10.1093/nar/gkaa669."
COMMENT     Sequence Label: pLenti-BLRR
FEATURES             Location/Qualifiers
     source          1..10295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..44
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     278..295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    412..433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(448..453,454..471,472..478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    486..502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     491..513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     510..526
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     510..526
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     545..565
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        547..565
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        593..819
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             820..1000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1047..1172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1665..1898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2083..2127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2533..2836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2837..3040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2991..3011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3037..3061
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3145..3169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3765..3818
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(5492..5516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5547..6135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5600..5620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6018..6029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6149..6648
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6184..6207)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6581..6600
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6669..7268
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6669..6688)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7165..7185
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7406..7639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7711..7832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7748..7767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7802..7821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7872..8007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8027..8046)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8028..8046)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8056..8073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8056..8072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8065..8087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8214..8669
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8301..8320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8511..8532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8695..8799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8800..8868,8869..9660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9018..9037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9831..10295,1..124)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
       61 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      301 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      361 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      421 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      481 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      541 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      601 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      661 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      721 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      781 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     1021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     1081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1501 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1561 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1621 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1681 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1741 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1801 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1861 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1921 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1981 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     2041 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2101 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2161 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2221 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2281 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2341 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc
     2521 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2581 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2641 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2701 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2761 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2821 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2881 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2941 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3001 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     3061 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagtccag
     3121 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc caccatggga
     3181 gtcaaagttc tgtttgccct gatctgcatc gctgtggccg aggccaagcc caccgagaac
     3241 aacgaagact tcaacatcgt ggccgtggcc agcaacttcg cgaccacgga tctcgatgct
     3301 gaccgcggga agttgcccgg caagaagctg ccgctggagg tgctcaaaga gatggaagcc
     3361 aatgcccgga aagctggctg caccaggggc tgtctgatct gcctgtccca catcaagtgc
     3421 acgcccaaga tgaagaagtt catcccagga cgctgccaca cctacgaagg cgacaaagag
     3481 tccgcagatc gaattaccct gttatcccta cacctataac atctcgcgat cgtcgacatt
     3541 cccgagattc ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat
     3601 ctgtgtgtgg actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttccgac
     3661 ctgctcaaga agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag
     3721 gtggacaaga tcaagggggc cggtggcgac tagcttattc cggtgagggc agaggaagtc
     3781 ttctaacatg cggtgacgtg gaggagaatc cgggccctat gaagataatt atcctttctg
     3841 tgattctggc ttactgtgtt acagtgaatt gtcaggatgc atgtccagta gaggcggaac
     3901 cgccatcttc taccccgacc gtaccaacct cctgcgaagc taaagaaggg gagtgcatcg
     3961 atacaaggtg cgctacctgc aaacgggata tcctgtccga cggactttgc gaaaataaac
     4021 ccgggaagac ctgctgtcga atgtgtcagt atgtcatcga atgccgggtc gaggccgccg
     4081 gttattttag aacattttac ggtaaacggt ttaatttcca ggaacccggc aaatacgtac
     4141 tggctcgcgg caccaagggt ggcgactgga gcgtcaccct gacaatggaa aacctggacg
     4201 ggcagaaagg agccgtgctt actaaaacta ccctggaggt ggcgggagac gtaattgaca
     4261 tcactcaggc aacggctgac ccaataaccg tgaacggagg agctgatccc gtgattgcaa
     4321 accctttcac tattggcgag gtcacgattg ccgtcgtcga aattccaggc ttcaacatca
     4381 cagtgatcga gttcttcaag ctgatcgtca ttgatatcct cggcggacgg tccgttcgca
     4441 tcgcacctga cacagccaac aagggcctga tctctggcat ttgtggtaac ttggaaatga
     4501 atgatgctga tgactttaca acggacgccg accaactggc tattcaacct aatatcaaca
     4561 aagagtttga tggatgtccc ttttacggaa atccttcaga catcgaatac tgcaaaggcc
     4621 tcatggaacc gtaccgggcc gtttgcagaa ataacatcaa cttctactat tatactctga
     4681 gctgcgcatt tgcatactgt atgggcggtg aggagagagc caaacatgtg cttttcgact
     4741 atgtggagac ctgcgccgcc ccggagactc gcggtacctg cgtcctgagc ggccatacct
     4801 tctatgacac cttcgataag gctaggtacc agttccaagg gccttgcaaa gagctcctga
     4861 tggccgcaga ttgttactgg aacacttggg acgtcaaagt ttcccatcgg gacgtagaga
     4921 gctacacgga agttgagaag gtgaccatca ggaagcagag taccgtcgta gacctgatcg
     4981 tcgacggcaa gcaggtaaag gtaggaggcg tggacgttag tattccgtat tcttctgaaa
     5041 atacgagcat ctactggcag gatggagaca ttctgacaac cgccatcctt ccagaagctc
     5101 tggtggtgaa gtttaacttc aagcagctgc tggtagtgca cattcgcgac ccattcgacg
     5161 ggaaaacctg tgggatttgc ggcaactaca accaggactc aactgacgat ttctttgacg
     5221 ccgaaggggc ttgcgctctt accccaaatc cgcctggatg caccgaagag caaaagcctg
     5281 aagcggaacg gctgtgcaat tcactgtttg attcttcaat agatgagaaa tgcaacgtgt
     5341 gttacaaacc tgaccgcatc gcacgctgca tgtatgagta ttgcctgaga ggtcaacaag
     5401 ggttctgcga tcacgcgtgg gaatttaaga aagaatgcta cataaagcac ggggatacat
     5461 tggaggtgcc gccagaatgc cagtagtcta gacccagctt tcttgtacaa agtggttgat
     5521 atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5581 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5641 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5701 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5761 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5821 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5881 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5941 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6001 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6061 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6121 gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6181 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6241 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     6301 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6361 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     6421 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6481 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6541 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     6601 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     6661 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6721 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6781 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6841 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6901 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6961 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7021 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7081 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7141 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7201 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7261 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     7321 catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7381 cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat
     7441 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7501 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7561 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7621 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7681 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7801 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7861 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7921 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7981 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8041 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8101 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8161 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8221 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8281 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8341 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8401 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8461 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8521 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8581 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8641 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8761 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8821 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8881 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8941 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9001 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9061 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9121 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9181 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9241 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9301 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9361 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9421 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9481 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9541 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9601 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9661 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9721 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9781 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9841 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9901 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9961 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10021 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10081 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10141 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10201 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10261 gacaggtatc cggtaagcgg cagggtcgga acagg
//
