LOCUS       sequence-307053-       11967 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses EGFP-MYO10-BioID construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11967)
  TITLE     bioRxiv--Proximity proteomics identifies Lamellipodin as a novel 
            Myosin-X cargo protein
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EGFP-MYO10-BioID
FEATURES             Location/Qualifiers
     source          1..11967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1345..1371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1372..1389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1390..1407
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1671..1680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1795..1804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6567..6576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7645..7992,7993..7995,7996..8604)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             8608..8634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8755..8876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8792..8811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8846..8865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8883..9338)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9020..9041)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9232..9251
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9365..9469
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9471..9828
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9471..9662
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9494..9514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9679..9814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9863..10657
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9863..10657
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9917..9936)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10527..10546
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10845..10864)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10889..10936
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11265..11853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11754..11773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atcctacccc tatgatgtgc ctgactatgc ccaccaccac
     1381 caccaccacc tggtgcccag gggcagcaga tctcgagctc aagcttcgaa ttctgcagtc
     1441 gacggtacca tggataactt cttcaccgag ggaacacggg tctggctgag agaaaatggc
     1501 cagcattttc caagtactgt aaattcctgt gcagaaggca tcgtcgtctt ccggacagac
     1561 tatggtcagg tattcactta caagcagagc acaattaccc accagaaggt gactgctatg
     1621 caccccacga acgaggaggg cgtggatgac atggcgtcct tgacagagct ccatggcggc
     1681 tccatcatgt ataacttatt ccagcggtat aagagaaatc aaatatatac ctacatcggc
     1741 tccatcctgg cctccgtgaa cccctaccag cccatcgccg ggctgtacga gcctgccacc
     1801 atggagcagt acagccggcg ccacctgggc gagctgcccc cgcacatctt cgccatcgcc
     1861 aacgagtgct accgctgcct gtggaagcgc tacgacaacc agtgcatcct catcagtggt
     1921 gaaagtgggg caggtaaaac cgaaagcact aaattgatcc tcaagtttct gtcagtcatc
     1981 agtcaacagt ctttggaatt gtccttaaag gagaagacat cctgtgttga acgagctatt
     2041 cttgaaagca gccccatcat ggaagctttc ggcaatgcga agaccgtgta caacaacaac
     2101 tctagtcgct ttgggaagtt tgttcagctg aacatctgtc agaaaggaaa tattcagggc
     2161 gggagaattg tagattattt attagaaaaa aaccgagtag taaggcaaaa tcccggggaa
     2221 aggaattatc acatatttta tgcactgctg gcagggctgg aacatgaaga aagagaagaa
     2281 ttttatttat ctacgccaga aaactaccac tacttgaatc agtctggatg tgtagaagac
     2341 aagacaatca gtgaccagga atcctttagg gaagttatta cggcaatgga cgtgatgcag
     2401 ttcagcaagg aggaagttcg ggaagtgtcg aggctgcttg ctggtatact gcatcttggg
     2461 aacatagaat ttatcactgc tggtggggca caggtttcct tcaaaacagc tttgggcaga
     2521 tctgcggagt tacttgggct ggacccaaca cagctcacag atgctttgac ccagagatca
     2581 atgttcctca ggggagaaga gatcctcacg cctctcaatg ttcaacaggc agtagacagc
     2641 agggactccc tggccatggc tctgtatgcg tgctgctttg agtgggtaat caagaagatc
     2701 aacagcagga tcaaaggcaa tgaggacttc aagtctattg gcatcctcga catctttgga
     2761 tttgaaaact ttgaggttaa tcactttgaa cagttcaata taaactatgc aaacgagaaa
     2821 cttcaggagt acttcaacaa gcatattttt tctttagaac aactagaata tagccgggaa
     2881 ggattagtgt gggaagatat tgactggata gacaatggag aatgcctgga cttgattgag
     2941 aagaaacttg gcctcctagc ccttatcaat gaagaaagcc attttcctca agccacagac
     3001 agcaccttat tggagaagct acacagtcag catgcgaata accactttta tgtgaagccc
     3061 agagttgcag ttaacaattt tggagtgaag cactatgctg gagaggtgca atatgatgtc
     3121 cgaggtatct tggagaagaa cagagataca tttcgagatg accttctcaa tttgctaaga
     3181 gaaagccgat ttgactttat ctacgatctt tttgaacatg tttcaagccg caacaaccag
     3241 gataccttga aatgtggaag caaacatcgg cggcctacag tcagctcaca gttcaaggac
     3301 tcactgcatt ccttaatggc aacgctaagc tcctctaatc ctttctttgt tcgctgtatc
     3361 aagccaaaca tgcagaagat gccagaccag tttgaccagg cggttgtgct gaaccagctg
     3421 cggtactcag ggatgctgga gactgtgaga atccgcaaag ctgggtatgc ggtccgaaga
     3481 ccctttcggg acttttacaa aaggtataaa gtgctgatga ggaatctggc tctgcctgag
     3541 gacgtccgag ggaagtgcac gagcctgctg cagctctatg atgcctccaa cagcgagtgg
     3601 cagctgggga agaccaaggt ctttcttcga gaatccttgg aacagaaact ggagaagcgg
     3661 agggaagagg aagtgagcca cgcggccatg gtgattcggg cccatgtctt gggcttctta
     3721 gcacgaaaac aatacagaaa ggtcctttat tgtgtggtga taatacagaa gaattacaga
     3781 gcattccttc tgaggaggag atttttgcac ctgaaaaagg cagccatagt tttccagaag
     3841 caactcagag gtcagattgc tcggagagtt tacagacaat tgctggcaga gaaaagggag
     3901 caagaagaaa agaagaaaca ggaagaggaa gaaaagaaga aacgggagga agaagaaaga
     3961 gaaagagaga gagagcgaag agaagccgag ctccgcgccc agcaggaaga agaaacgagg
     4021 aagcagcaag aactcgaagc cttgcagaag agccagaagg aagctgaact gacccgtgaa
     4081 ctggagaaac agaaggaaaa taagcaggtg gaagagatcc tccgtctgga gaaagaaatc
     4141 gaggacctgc agcgcatgaa ggagcagcag gagctgtcgc tgaccgaggc ttccctgcag
     4201 aagctgcagg agcggcggga ccaggagctc cgcaggctgg aggaggaagc gtgcagggcg
     4261 gcccaggagt tcctcgagtc cctcaatttc gacgagatcg acgagtgtgt ccggaatatc
     4321 gagcggtccc tgtcggtggg aagcgaattt tccagcgagc tggctgagag cgcatgcgag
     4381 gagaagccca acttcaactt cagccagccc tacccagagg aggaggtcga tgagggcttc
     4441 gaagccgacg acgacgcctt caaggactcc cccaacccca gcgagcacgg ccactcagac
     4501 cagcgaacaa gtggcatccg gaccagcgat gactcttcag aggaggaccc atacatgaac
     4561 gacacggtgg tgcccaccag ccccagtgcg gacagcacgg tgctgctcgc cccatcagtg
     4621 caggactccg ggagcctaca caactcctcc agcggcgagt ccacctactg catgccccag
     4681 aacgctgggg acttgccctc cccagacggc gactacgact acgaccagga tgactatgag
     4741 gacggtgcca tcacttccgg cagcagcgtg accttctcca actcctacgg cagccagtgg
     4801 tcccccgact accgctgctc tgtggggacc tacaacagct cgggtgccta ccggttcagc
     4861 tctgaggggg cgcagtcctc gtttgaagat agtgaagagg actttgattc caggtttgat
     4921 acagatgatg agctttcata ccggcgtgac tctgtgtaca gctgtgtcac tctgccgtat
     4981 ttccacagct ttctgtacat gaaaggtggc ctgatgaact cttggaaacg ccgctggtgc
     5041 gtcctcaagg atgaaacctt cttgtggttc cgctccaagc aggaggccct caagcaaggc
     5101 tggctccaca aaaaaggggg gggctcctcc acgctgtcca ggagaaattg gaagaagcgc
     5161 tggtttgtcc tccgccagtc caagctgatg tactttgaaa acgacagcga ggagaagctc
     5221 aagggcaccg tagaagtgcg aacggcaaaa gagatcatag ataacaccac caaggagaat
     5281 gggatcgaca tcattatggc cgataggact ttccacctga ttgcagagtc cccagaagat
     5341 gccagccagt ggttcagcgt gctgagtcag gtccacgcgt ccacggacca ggagatccag
     5401 gagatgcatg atgagcaggc aaacccacag aatgctgtgg gcaccttgga tgtggggctg
     5461 attgattctg tgtgtgcctc tgacagccct gatagaccca actcgtttgt gatcatcacg
     5521 gccaaccggg tgctgcactg caacgccgac acgccggagg agatgcacca ctggataacc
     5581 ctgctgcaga ggtccaaagg ggacaccaga gtggagggcc aggaattcat cgtgagagga
     5641 tggttgcaca aagaggtgaa gaacagtccg aagatgtctt cactgaaact gaagaaacgg
     5701 tggtttgtac tcacccacaa ttccctggat tactacaaga gttcagagaa gaacgcgctc
     5761 aaactgggga ccctggtcct caacagcctc tgctctgtcg tccccccaga tgagaagata
     5821 ttcaaagaga caggctactg gaacgtcacc gtgtacgggc gcaagcactg ttaccggctc
     5881 tacaccaagc tgctcaacga ggccacccgg tggtccagtg ccattcaaaa cgtgactgac
     5941 accaaggccc cgatcgacac ccccacccag cagctgattc aagatatcaa ggagaactgc
     6001 ctgaactcgg atgtggtgga acagatttac aagcggaacc cgatccttcg atacacccat
     6061 caccccttgc actccccgct cctgcccctt ccgtatgggg acataaatct caacttgctc
     6121 aaagacaaag gctataccac ccttcaggat gaggccatca agatattcaa ttccctgcag
     6181 caactggagt ccatgtctga cccaattcca ataatccagg gcatcctaca gacagggcat
     6241 gacctgcgac ctctgcggga cgagctgtac tgccagctta tcaaacagac caacaaagtg
     6301 ccccaccccg gcagtgtggg caacctgtac agctggcaga tcctgacatg cctgagctgc
     6361 accttcctgc cgagtcgagg gattctcaag tatctcaagt tccatctgaa aaggatacgg
     6421 gaacagtttc caggaaccga gatggaaaaa tacgctctct tcacttacga atctcttaag
     6481 aaaaccaaat gccgagagtt tgtgccttcc cgagatgaaa tagaagctct gatccacagg
     6541 caggaaatga catccacggt ctattgccat ggcggcggct cctgcaagat caccatcaac
     6601 tcccacacca ctgctgggga ggtggtggag aagctgatcc gaggcctggc catggaggac
     6661 agcaggaaca tgtttgcttt gtttgaatac aacggccacg tcgacaaagc cattgaaagt
     6721 cgaaccgtcg tagctgatgt cttagccaag tttgaaaagc tggctgccac atccgaggtt
     6781 ggggacctgc catggaaatt ctacttcaaa ctttactgct tcctggacac agacaacgtg
     6841 ccaaaagaca gtgtggagtt tgcatttatg tttgaacagg cccacgaagc ggttatccat
     6901 ggccaccatc cagccccgga agaaaacctc caggttcttg ctgccctgcg actccagtat
     6961 ctgcaggggg attatactct gcacgctgcc atcccacctc tcgaagaggt ttattccctg
     7021 cagagactca aggcccgcat cagccagtca accaaaacct tcaccccttg tgaacggctg
     7081 gagaagaggc ggacgagctt cctagagggg accctgaggc ggagcttccg gacaggatcc
     7141 gtggtccggc agaaggtcga ggaggagcag atgctggaca tgtggattaa ggaagaagtc
     7201 tcctctgctc gagccagtat cattgacaag tggaggaaat ttcagggaat gaaccaggaa
     7261 caggccatgg ccaagtacat ggccttgatc aaggagtggc ctggctatgg ctcgacgctg
     7321 tttgatgtgg agtgcaagga aggtggcttc cctcaggaac tctggttggg tgtcagcgcg
     7381 gacgccgtct ccgtctacaa gcgtggagag ggaagaccac tggaagtctt ccagtatgaa
     7441 cacatcctct cttttggggc acccctggcg aatacgtata agatcgtggt cgatgagagg
     7501 gagctgctct ttgaaaccag tgaggtggtg gatgtggcca agctcatgaa agcctacatc
     7561 agcatgatcg tgaagaagcg ctacagcacg acacgctccg ccagcagcca gggcagctcc
     7621 agggtaaggc ggtctagagg atccaaggac aacaccgtgc ccctgaagct gatcgccctg
     7681 ctggccaacg gcgagttcca ctctggcgag cagctgggag agaccctggg aatgagcaga
     7741 gccgccatca acaagcacat ccagacactg agagactggg gagtggacgt gttcaccgtg
     7801 cctggcaagg gctacagcct gcctgagcct atccagctgc tgaacgccaa gcagatcctg
     7861 ggacagctgg atggcggaag cgtggccgtg ctgcctgtga tcgactccac caatcagtac
     7921 ctgctggaca gaatcggaga gctgaagtcc ggcgacgcct gcatcgccga gtaccagcag
     7981 gctggcagag gaggcagagg acggaagtgg ttcagcccat tcggagccaa cctgtacctg
     8041 tccatgttct ggagactgga gcagggacct gctgctgcca tcggactgag tctggtgatc
     8101 ggaatcgtga tggccgaggt gctgagaaag ctgggagccg acaaggtgag agtgaagtgg
     8161 cctaatgacc tgtacctcca ggaccgcaag ctggctggca tcctggtgga gctgacaggc
     8221 aagacaggcg atgccgctca gatcgtgatc ggagccggaa tcaacatggc catgagaaga
     8281 gtggaggaga gcgtggtgaa ccagggctgg atcaccctgc aggaggctgg catcaacctg
     8341 gaccggaaca ccctggccgc catgctgatc agagagctga gagccgctct ggagctgttc
     8401 gagcaggagg gactggctcc ttacctgagc agatgggaga agctggacaa cttcatcaac
     8461 agacctgtga agctgatcat cggcgacaag gaaatcttcg gcatctccag aggaatcgac
     8521 aagcagggag ctctgctgct ggagcaggac ggaatcatca agccctggat gggcggagaa
     8581 atctccctga gaagcgcaga gaaggcttac ccttacgatg taccggatta cgcatagtct
     8641 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8701 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     8761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     8881 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     8941 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     9001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     9061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     9121 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     9181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     9241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     9301 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     9361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     9481 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9781 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     9841 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     9901 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     9961 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    10021 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
    10081 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    10141 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    10201 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    10261 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    10321 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    10381 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    10441 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    10501 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    10561 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    10621 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    10681 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    10741 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    10801 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
    10861 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
    10921 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
    10981 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
    11041 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
    11101 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    11221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    11281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    11341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    11401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    11461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    11521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11941 gtggataacc gtattaccgc catgcat
//
