LOCUS       sequence-307161-       11897 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full 
            length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLenti PGK mKate2-OGT 5N5A (Puro)
FEATURES             Location/Qualifiers
     source          1..11897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4183..4693
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4612..4630
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4748..4772
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4803..5498
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             5505..5528
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5532..5555
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    5571..5595
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8720..8744)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8775..9363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8828..8848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9246..9257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9377..9876
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9412..9435)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9809..9828
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9897..10496
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9897..9916)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10393..10413
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10634..10867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10939..11060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10976..10995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11030..11049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11100..11235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11255..11274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11256..11274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11284..11301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11284..11300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11293..11315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11442..11870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11529..11548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11739..11760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     4141 gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc caccggttcg tcgactagtc cagtgtggtg gaattctgca
     4741 gatatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag tcgactggat
     4801 ccatggtgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     4861 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     4921 agaccatgag aatcaaggcg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     4981 ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact
     5041 tctttaagca gtccttcccc gagggcttca catgggagag agtcaccaca tacgaagacg
     5101 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     5161 tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
     5221 gctgggaggc ctccaccgag accctgtacc ccgctgacgg cggcctggaa ggcagagccg
     5281 acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca
     5341 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac
     5401 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg
     5461 ccagatactg cgacctccct agcaaactgg ggcacagagg cggagactac aaagatgatg
     5521 acgacaaagg cgactacaaa gacgatgacg acaagggcat cgatagatca acaagtttgt
     5581 acaaaaaagc aggctttgcg tcttccgtgg gcaacgtggc cgacagcaca gggttagctg
     5641 agttggcaca tcgagaatat caggcaggag attttgaggc agctgagaga cactgcatgc
     5701 agctctggag acaagagcca gacaatactg gtgtgctttt attactttca tctatacact
     5761 tccagtgtcg aaggctggac agatctgctc actttagcac tctggcaatt aaacagaacc
     5821 cccttctggc agaagcttat tcgaatttgg ggaatgtgta caaggaaaga gggcagttgc
     5881 aggaggcaat tgagcattat cgacatgcat tgcgtctcaa acctgatttc atcgatggtt
     5941 atattaacct ggcagccgcc ttggtagcag cgggtgacat ggaaggggca gtacaagctt
     6001 acgtctctgc tcttcagtac aatcctgatt tgtactgtgt tcgcagtgac ctggggaacc
     6061 tgctcaaagc cctgggtcgc ttggaagaag ccaaggcatg ttatttgaaa gcaattgaga
     6121 cgcaaccgaa ctttgcagta gcttggagta atcttggctg tgttttcaat gcacaagggg
     6181 aaatttggct tgcaattcat cactttgaaa aggctgtcac ccttgaccca aactttctgg
     6241 atgcttatat caatttagga aatgtcttga aagaggcacg catttttgac agagctgtgg
     6301 cagcttatct tcgtgcccta agtttgagtc caaatcacgc agtggtgcac ggcaacctgg
     6361 cttgtgtata ctatgagcaa ggcctgatag atctggcaat agacacctac aggcgggcta
     6421 tcgaactaca accacatttc cctgatgctt actgcaacct agccaatgct ctcaaagaga
     6481 agggcagtgt tgctgaagca gaagattgtt ataatacagc tctccgtctg tgtcccaccc
     6541 atgcagactc tctgaatgca ctagccaata tcaaacgaga acagggaaac attgaagagg
     6601 cagttcgctt gtatcgtaaa gcattagaag tcttcccaga gtttgctgct gcccattcag
     6661 cattagcaag tgtactgcag cagcagggaa aactgcagga agctctgatg cattataagg
     6721 aggctattcg aatcagtcct acctttgctg atgcctactc tgcaatggga aacactctaa
     6781 aggagatgca ggatgttcag ggagccttgc agtgttatac gcgtgccatc caaattaatc
     6841 ctgcatttgc agatgcacat agcgcactgg cttccattca taaggattca gggaatattc
     6901 cagaagccat agcttcttac cgcacggctc tgaaacttaa gcctgatttt cctgatgctt
     6961 attgtgcatt ggctcattgc ctgcagattg tctgtgattg gacagactat gatgagcgaa
     7021 tgaagaagtt ggtcagtatt gtggctgacc agttagagaa gaataggttg ccttctgtgc
     7081 atcctcatca tagtatgcta tatcctcttt ctcatggctt caggaaggct attgctgaga
     7141 ggcacggcaa cctgtgctta gataagatta atgttcttca taaaccacca tatgaacatc
     7201 caaaagactt gaagctcagt gatggtcggc tgcgtgtagg atatgtgagt tccgactttg
     7261 ggaatcatcc tacttctcac cttatgcagt ctattccagg catgcacaat cctgataaat
     7321 ttgaggtgtt ctgttatgcc ctgagcccag acgatggcac aaacttccga gtgaaggtga
     7381 tggcagaagc caatcatttc attgatcttt ctcagattcc atgcaatgga aaagcagctg
     7441 atcgcatcca tcaggatgga attcatatcc ttgtaaatat gaatggctat actaagggcg
     7501 ctcgaaatga gctttttgct ctcaggccag ctcctattca ggcaatgtgg ctgggatacc
     7561 ctgggacgag tggtgcgctt ttcatggatt atattatcac tgatcaggaa acttcgccag
     7621 ctgaagttgc tgagcagtat tccgagaaat tggcttatat gccccacact ttttttattg
     7681 gtgatcatgc taatatgttc cctcacctga agaaaaaagc agtcatcgat tttaagtcca
     7741 atgggcacat ttatgacaat cggatagttc tgaatggcat cgacctcaaa gcatttcttg
     7801 atagtctacc agatgtgaaa attgtcaaga tgaagtgtcc tgatggagga gacaatgcag
     7861 atagcagtaa cacagctctt aatatgcctg ttattcctat gaatactatt gcagaagcag
     7921 ttattgaaat gattaaccga ggacagattc aaataacaat taatggattc agtattagca
     7981 atggactggc aactactcag atcaacaata aggctgcaac tggagaggag gttccccgta
     8041 ccattattgt aaccacccgt tctcagtacg ggttaccaga agatgccatc gtatactgta
     8101 actttaatca gttgtataaa attgaccctt ctactttgca gatgtgggca aacattctga
     8161 agcgtgttcc caatagtgta ctctggctgt tgcgttttcc agcagtagga gaacctaata
     8221 ttcaacagta tgcacaaaac atgggcctgc cccagaaccg tatcattttt tcacctgttg
     8281 ctcctaaaga ggaacacgtc aggagaggcc agctggctga tgtctgcttg gacactccac
     8341 tctgtaatgg gcacaccaca gggatggatg tcctctgggc agggaccccc atggtgacta
     8401 tgccaggaga gactcttgct tctcgagttg cagcatccca gctcacttgc ttaggttgtc
     8461 ttgagcttat tgctaaaaac agacaagaat atgaagacat agctgtgaag ctgggaactg
     8521 atctagaata cctgaagaaa gttcgtggca aagtctggaa gcaaagaata tctagccctc
     8581 tgttcaacac caaacaatac acaatggaac tagagcggct ctatctacag atgtgggagc
     8641 attatgcagc tggcaacaaa cctgaccaca tgattaagcc tgttgaagtc actgagtcag
     8701 cataactcga gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt
     8761 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8821 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8881 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8941 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     9001 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     9061 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     9121 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     9181 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9241 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9301 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9361 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     9421 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     9481 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     9541 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     9601 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     9661 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     9721 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     9781 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     9841 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     9901 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     9961 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
    10021 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
    10081 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
    10141 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
    10201 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
    10261 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
    10321 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
    10381 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
    10441 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
    10501 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
    10561 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    10621 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
    10681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10861 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
    10921 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    11041 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
    11101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    11161 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    11221 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
    11281 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
    11341 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
    11401 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
    11461 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    11521 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    11581 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    11641 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    11701 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    11761 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    11821 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    11881 attaacgctt acaattt
//
