LOCUS       sequence-307208-        1025 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to 
            mScarlet fluorophores in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Bruni GN, Kralj JM
  TITLE     Membrane voltage dysregulation driven by metabolic dysfunction 
            underlies bactericidal activity of aminoglycosides.
  JOURNAL   eLife. 2020 Aug 4;9. pii: 58706. doi: 10.7554/eLife.58706.
  PUBMED    32748785
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-08-4"; volume: "9. pii: 58706. doi"; pages: " 
            10.7554/eLife.58706"
COMMENT     Sequence Label: pKL09-GCaMP6f-mScarlet bb118
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..29
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     226..243
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 annacgancg cagcgagtca gtgagcgagg aagccaaggc ccagtctttc gactgagcct
       61 ttcgttttat ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca
      121 tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta
      181 ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc
      241 aggctgaaaa tcttctctca tccgccaaaa cagccaagct ggagaccgtt taaactcact
      301 tgtacaattc gtccatcccc ccggtagagt ggcgaccctc actgcgctcg tattgttcta
      361 caactgtata atcttcatta tgcgaagtga tatctaactt acgatctacg ttatatgcgc
      421 ctggcatctg aacaggtttc tttgccttgt acgtggtctt gaagtccgct aaataacgcc
      481 cgccgtcctt caggcgaagt gccattttga tatccccttt caggacgcca tcctctggat
      541 ataagcgctc cgtactggct tcccagccca tagttttctt ttgcataact ggaccgtcgg
      601 gaggaaaatt cgtcccacgc aactttacct tgtagatcaa tgttccatcc tccaaactgg
      661 tgtcttgcgt cacggtcaca gctccgccgt cctcgaaatt catcacacgt tcccacttaa
      721 atccctcggg aaaagattgt ttgtaataat ccgggatatc ggccgggtgc ttcgtgaaag
      781 cgcggctccc gtacataaat tgggganaaa ggatgtccca agaaaagggc aaaggtcccc
      841 ccttggngac cttcaacttg gcggtctgtg tcccttcgta cggacgcccc tcancctcac
      901 cctcaatctc aaattcgtgt ccgttcattg aacccnncnt nnnntacttt aaacgcatga
      961 actcntnnng natccaccgc tncnancgct accncngatc cnnccttcnc tgncatcntt
     1021 tgtac
//
