LOCUS       sequence-307883-       21492 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Human INS targeting vector for knockin of 2A separated luciferase, 
            humand CD19, and GFP. Targeted cells will be puromycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21492)
  AUTHORS   Ma H, Jeppesen JF, Jaenisch R
  TITLE     Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into
            Mechanisms of Human CD19-Positive beta Cell Destruction.
  JOURNAL   Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
  PUBMED    33205073
REFERENCE   2  (bases 1 to 21492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2020-09-22"; volume: "1(6):100097. doi"; pages: " 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22"
COMMENT     Sequence Label: INS-2A-luciferase-2A-CD19-GFP
FEATURES             Location/Qualifiers
     source          1..21492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    15..136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(52..71)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     106..125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    181..214
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        636..913
                     /label=chicken beta-actin promoter
     intron          914..1930
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1854..1876
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1938..1957
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1991..2590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1991..2010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2487..2507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2607..2626
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2769..2786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2775..2999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3056..3089
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7151..7213
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           7235..7274
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             7292..8422
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(8420..8439)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8464..8512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(9086..9104)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9125..9141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9125..9141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9138..9160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9149..9165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9173..9179,9180..9197,9198..9203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9218..9239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9356..9373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9527..10115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9607..9626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10286..11077,11078..11146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10909..10928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11147..11251)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(11446..11550)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11577..12032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11664..11683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11874..11895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12158..12180
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12172..12189
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12173..12189
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12196..12215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12196..12214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      15071..15080
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     16105..16176
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
     CDS             17228..17254
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             17264..17317
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             17318..18967
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTLVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(17384..17402)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             18974..19027
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             20714..20767
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(20768..20770,20771..21484)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(20768..20770,20771..21481)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(20810..20831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(21071..21090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
       61 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      121 tcatcaatgt atcttaaaat tcctgcagcc caattccgat catattcaat aacccttaat
      181 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca
      241 gccaagctag cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
      661 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
      721 attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg
      781 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
      841 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      901 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      961 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     1021 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     1081 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     1141 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     1201 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     1261 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     1321 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     1381 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     1441 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     1501 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     1561 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     1621 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     1681 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     1741 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1801 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1861 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     1921 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     1981 ccggtccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     2041 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     2101 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     2161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     2221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     2281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     2341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     2401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     2461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     2521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     2581 cggtgcctga cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     2641 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     2701 catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattct ctagagctcg
     2761 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2821 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2881 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2941 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3001 ttctgaggcg gaaagaacca gctggggctc gactagagct tgcggaaccc ttaatataac
     3061 ttcgtataat gtatgctata cgaagttatg aattcgccac tgagaagtga cttcttgttc
     3121 agtagctctg gactcttgga gtccccagag accttgttca ggaaagggaa tgagaacatt
     3181 ccagcaattt tccccccacc tagccctccc aggttctatt tttagagtta tttctgatgg
     3241 agtccctgtg gagggaggag gctgggctga gggagggggt cctgcagggc ggggggctgg
     3301 gaaggtgggg agaggctgcc gagagccacc cgctatcccc agctctgggc agccccggga
     3361 cagtcacaca ccctggcctc gcggcccaag ctggcagccg tctgcagcca cagcttatgc
     3421 cagcccaggt ccagccagac acctgaggga cccactggtg ccttggagga agcaggagag
     3481 gtcagatggc accatgagct ggggcaggtg cagggaccgt ggcagcacct ggcagggcct
     3541 cagaacccat gccttgggca ccccggccat gaggccctga ggattgcagc ccaggagaag
     3601 cagggaaccg ccagggccac aggggcagag accagggcca gggtccccct gcagcccctt
     3661 agcccacccc ctcccagtaa gcagggctgc ttggctggct tcctttgcta cagacctgct
     3721 gctcacccag aagggcccac gggccctggt gacaaggtcg ttgtggctcc aggtccttgg
     3781 gggtcctgac acagagcctc ttctgcagca cccctgagga cagggtggct ccgctgggca
     3841 cccagcctag tgggcagacg agaacctagg ggctgcctgg gcctactgtg gcctgggagg
     3901 tcagcgggtg accctagcta ccctgtggct gggccagtct gcctgccacc caggccaaac
     3961 caatctgcac ctttcctgag agctccaccc agggctgggc tggggatggc tgggcctggg
     4021 gctggcatgg gctgtggctg cagaccactg ccagcttggg cctcgaggcc aggagctcac
     4081 cctccagctg gggacctggc cactggggca gccctgttcc tgaagctctg agctcacccc
     4141 ttccccatga ccacatcagc ccccctccac ccagagatgt cacagccccc agctagcccc
     4201 gcctccagag tgggggccaa ggctgggcag gcgggtggac ggccggacac tggccccgga
     4261 agaggaggga ggcggtggct gggatcggca gcagccgtcc atgggaacac ccagccggcc
     4321 ccactcgcac gggtagagac aggggcgccc tgctggagct gaggtatgtg agctcgcgcg
     4381 gggctgggcc aaagcggggc ccggtgggcc ggctgggagg ctgcccacca gtcagccatc
     4441 ggccaagctg ttgccctggc tgaccctgat ggccaacaag gccgtaggga gtgatgggca
     4501 gaggcccctt ctgggagggg agggtcagtg ctctgtgggg gaccgtgtgt tggagtggag
     4561 ggcagcagga ggagcccttt ggtgtccagg gactcctgga gctgccccag ccttccagga
     4621 cttgcagggc agctggcact ggctggtgct gggggctgag gagtgtcttt tgaggggcca
     4681 aattttctgt gacttctgtc ctgggggacc tctgacctga ggcctcagga gagggcaagg
     4741 ccgcccaccc aaaagagatg cagccatggt tcgcggtgcc ctcggctgcc ctgggccaga
     4801 gctggggcta gctttcacct tgttgagacc caggactctg tcccccaagc ctgtcttcgc
     4861 cagcgccttg accccacccc tcatatactg tgtcctggaa aacgtggaca cgggagaccg
     4921 cagccagggc gaggtatcgc ccctccatcc ccccaggccc aatgagaagc agttggccaa
     4981 ggtgatccag gtggcagagg cagcatcaga cccagtctcc tgtcaggcac caccttgggt
     5041 gccggtcccc agatgccctg gcggggagtg tgcatgctcc cggagccccc aggtcacccc
     5101 atgtgagcca ggcccacaga gcttggctct gcaatgcctg ctgggctgct gcccatgctc
     5161 caccccttct gggaagctaa aagacagccc ttcagtgtcc agagacctgc ctggccttgg
     5221 agcctcggtt tcacatgccc accgggctgg caggggcact cagctgcctc cagccccggc
     5281 ggtcaccctg gcattgggtc catctaactg ctccccagtc acaaggcagc tgctccccaa
     5341 gtctccccaa acctgctggc ccctctagaa gcctctgtcc attcctggag gaccgagggc
     5401 agcctgcatg ccatcccgca cacagccttc tgtctgggcg tcctgccttc acacatgctg
     5461 cacagggagg aaactcttat accacattcc ttaagcagag actgaagcct ggagccaggc
     5521 acatggcacg tgctcccacc cacccaggac acactgcggt gtggctgcct ccaggctggc
     5581 cccctagatt gcgtctgctc ctggcatgga taactggcgc ctttgcctgg ccgttggggc
     5641 agtgtttgcc ttcccctgtc ggcagcaaat atttactgtc ctccgtctcc aggactctcc
     5701 aggcctgagc agaccccggg gggatgagtg tggactcagc ggtgctgagg gtagccccct
     5761 gcccttcggg tcctggtgcc cagcaggggt ccagcccagg gaagagactg aggccaggac
     5821 aggcagtgtt taagcctgag tttctgggaa aggtagccct gggcagaact tgggccgaac
     5881 gttggccagt gtctctctcc agccaggctg tgaggtagct gtttccagga tgggcacctt
     5941 tccacaccca gcaatgtggc caggagccgc cattcacggg tgcgaccagc agatggcatc
     6001 agagcctcac ttttgatgca ctccggccac cagccacggg tccaggttct ggccaccacc
     6061 cagggtctga gcagctgcat cctgcccctg ccgggcactc ccgggggctg tggggcctgt
     6121 gggggccctg ccagacactc ttgggggctg tggggggccc tgccaggcac tcccagggac
     6181 tatgggggct gtggggggcc ctgctgggca ctctgaaggg catggggctt aggaatgaga
     6241 ggagctgtct gatgatgatg gtgggggcat tgcagaggcc cccggcctgc tcaggtccag
     6301 tctcggcccc taagtcaagc ctcaggccag cctctcacca gcctgggttt ctcagagggc
     6361 cgggacaaat gttctgggtc tctaatattc caagaaagcc tctggctgga ctctgagccc
     6421 cacctgcgag cccctagaat cacagagagc tagggtgaga agaccagggg gactccgtcc
     6481 caccctcgtc gtggctgagc ccactgtggc cggtggtgga ccaggctgtg gcctttgctg
     6541 agggtcccca gggcccctgg gggctactga ggctggaggc cagcggtggc caggagggtc
     6601 cctccctcag ccactcaagc cagaaggtcg agtcctggtt tctatgtgag gagggggctt
     6661 caggggctgg gacctggggg caccgaaggc ctggagctgg ggtccaggcg gctgagggtt
     6721 agtgcgttcc cacgctcccc tccgccagcg ccgtgaggag agggaggtcc actctggaaa
     6781 gaatgtttga gggcaggggt agacagggtc tgggaacgcg gagatgacac actggggtgc
     6841 cgcctccgca ccctccgccc ttcctcccac tattgtttct ggcctcggcc gcaggaacag
     6901 caaggcagac tagtggatcc tctagagtcg agcagtgtgg ttttcaagag gaagcaaaaa
     6961 gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcagtgtgg ttttgcaaga
     7021 ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcaaaccc
     7081 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     7141 ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     7201 gcgacccgct taacagcgtc aacagcgtgc cgcagatctt ggtggcgtga aactcccgca
     7261 cctcttcggc cagcgccttg tagaagcgcg tatggcttcg taccccggcc atcagcacgc
     7321 gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta cggcgttgcg
     7381 ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc ccacgctact
     7441 gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc aactgctggt
     7501 ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcgggt
     7561 gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc ttgaccaggg
     7621 tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat
     7681 gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc
     7741 acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct
     7801 gtgctacccg gccgcgcgat accttatggg cagcatgacc ccccaggccg tgctggcgtt
     7861 cgtggccctc atcccgccga ccttgcccgg cacaaacatc gtgttggggg cccttccgga
     7921 ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc
     7981 tatgctggcc gcgattcgcc gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca
     8041 gggcggcggg tcgtggcggg aggattgggg acagctttcg gggacggccg tgccgcccca
     8101 gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac
     8161 cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtacaacg tgtttgcctg
     8221 ggccttggac gtcttggcca aacgcctccg tcccatgcac gtctttatcc tggattacga
     8281 ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga tgatccagac
     8341 ccacgtcacc accccaggct ccataccgac gatctgcgac ctggcgcgca cgtttgcccg
     8401 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     8461 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     8521 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8581 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8641 ctcgcagcca acgtcggggc ggcaggccct gccatagcca cgggccccgt gggttaggga
     8701 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     8761 ggggtcagtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
     8821 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
     8881 cccccaaaaa ccaccgcgcg gatttctggc gccgccggac gaactaaacc tgactacggc
     8941 atctctgccc cttcttcgct ggtacgagga gcgcttttgt tttgtattgg tcaccacggc
     9001 cgagtttccg cgggaccccg gccagatcaa gctagcttat cgataccgtc gatcgacctc
     9061 gagggggggc ccgcagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     9121 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctctca
    11281 aggatcttac cgctgttgag atccagttcg atgtaaccca ttcgtgcacc caacttgatc
    11341 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11401 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
    11581 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    11641 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    11701 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    11761 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    11821 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    11881 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    11941 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    12001 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt
    12061 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    12121 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    12181 acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtgga
    12241 tgcatgtcga caaggaaaaa agcggccgcc cctctagcct catcctcata aaaggtctca
    12301 tcattttccc tccagcctct tatgcactgg ggaaactgag gccaggggct atgtgtccag
    12361 cggacagggg tgctgaattc cacccacagg cttagggata tggtcaagga aagcttcctg
    12421 gaggaggccc agtggaggtt cagggaggga tggggtgccc ggcagtctct agtggaaaag
    12481 gcgcctagcc tatctccccc atgaaccccc tcacccagcc ctggaagagg cctcagtgtc
    12541 ccgcctgtga ccagttggct cagaaaagcc ctgggagctc tgagccactg tgaaggtgga
    12601 aacgcggccc ctggcctccc ctctcctgga ggctgcagac tctgcccgcc agttgacgag
    12661 ggctctgccg ctctcctccc caggagctat gcctcacgca tccagcgccc cttctccgtg
    12721 aagttcgacc cgtacacgct ggccatcgac gtgctggaca gcccccaggc cgtgcggcgc
    12781 tccctggagg gtgtccagga tgagctggac acccttgccc atgcgctgag tgccattggc
    12841 taggtgcacg gcgtccctga gggcccttcc caacctcccc tggtcctgca ctgtcccgga
    12901 gctcaggccc tggtgagggg ctgggtcccg ggtgcccccc atgccctccc tgctgccagg
    12961 ctcccactgc ccctgcacct gcttctcagc gcaacagctg tgtgtgcccg tggtgaggtt
    13021 gtgctgcctg tggtgaggtc ctgtcctggc tcccagggtc ctgggggctg ctgcactgcc
    13081 ctccgccctt ccctgacact gtctgctgcc ccaatcaccg tcacaataaa agaaactgtg
    13141 gtctctacac ctgcctggcc ccacatctgt gccacagaga cagaccctgg gatcctcaga
    13201 ctcccacacc cccaccccag cctcactcag aggtttcgcc ctggcctcct tcctcctctg
    13261 ggagatggct ggccgccctg gccaggcagc tggcccctcc gggcctggtt tccccgctca
    13321 ccctgaggcc ccgcccagct ctgagcccca agcagctcca gaggctcggg caccctggcc
    13381 gagctgcccc atctccgtgg ggtgccctcc caaggtgggg agccacgtga cagtgggagg
    13441 gcctctctca ggcctggcag ggagcagggg tcacaaactg tgctggctgg gggtggtctc
    13501 agaggtgggc ctgcaggcct aaccctccct gctgacaggg ctcccagccc ttgagagaaa
    13561 cagggatgga ggaacagctg ccctgatgcc ctcacccacc cggagcaggc cctgcgaacc
    13621 aaggggaacc tcagtgtggc ccccagcatg tgtgctgatg gggagggtct ggctgagctg
    13681 gtgcccaggc agatggtctg ggcctgtctc cccagcgagg caggatgggg gctggatttc
    13741 agactctgta agatgcccct ggcttactcg aggggcctgg acattgccct ccagagagag
    13801 cacccaacac cctccaggct tgaccggcca gggtgtcccc ttcctacctt ggagagagca
    13861 gccccagggc atcctgcagg gggtgctggg acaccagctg gccttcaagg tctctgcctc
    13921 cctccagcca ccccactaca cgctgctggg atcctggatc tcagctcccc ggccgacaac
    13981 actggcaaac tcctactcat ccacgaaggc cctcctgggc atggtggtcc ttcccagcct
    14041 ggcagtctgt tcctcacaca ccttgttagt gcccagcccc tgaggttgca gctgggggtg
    14101 tctctgaagg gctgtgagcc cccaggaagc cctggggaag tgcctgcctt gcctcccccc
    14161 ggccctgcca gcgcctggct ctgccctcct acctgggctc cccccatcca gcctccctcc
    14221 ctacacactc ctctcaagga ggcacccatg tcctctccag ctgccgggcc tcagagcact
    14281 gtggcgtcct ggggcagcca ccgcatgtcc tgctgtggca tggctcaggg tggaaagggc
    14341 ggaagggagg ggtcctgcag atagctggtg cccactacca aacccgctcg gggcaggaga
    14401 gccaaaggct gggtgtgtgc agagcggccc cgagaggttc cgaggctgag gccagggtgg
    14461 gacataggga tgcgaggggc cggggcacag gatactccaa cctgcctgcc cccatggtct
    14521 catcctcctg cttctgggac ctcctgatcc tgcccctggt gctaagaggc aggtaggggc
    14581 tgcaggcagc agggctcgga gcccatgccc cctcaccatg ggtcaggctg gacctccagg
    14641 tgcctgttct ggggagctgg gagggccgga ggggtgtacc ccaggggctc agcccagatg
    14701 acactatggg ggtgatggtg tcatgggacc tggccaggag aggggagatg ggctcccaga
    14761 agaggagtgg gggctgagag ggtgcctggg gggccaggac ggagctgggc cagtgcacag
    14821 cttcccacac ctgcccaccc ccagagtcct gccgccaccc ccagatcaca cggaagatga
    14881 ggtccgagtg gcctgctgag gacttgctgc ttgtccccag gtccccaggt catgccctcc
    14941 ttctgccacc ctggggagct gagggcctca gctggggctg ctgtcctaag gcagggtggg
    15001 aactaggcag ccagcaggga ggggacccct ccctcactcc cactctccca cccccaccac
    15061 cttggcccat ccatggcggc atcttgggcc atccgggact ggggacaggg gtcctgggga
    15121 caggggtgtg gggacagggg tcctggggac aggggtctgg ggacaggggt cctggggaca
    15181 ggggtgtggg gacaggggtg tggggacagg ggtgtgggga caggggtcct ggggacaggg
    15241 gtctggggac aggggtctga ggacaggggt gtggggacag gggtgtgggg acaggggtgt
    15301 ggggacaggg gtgtggggac aggggtctgg ggacaggggt ccgggggaca ggggtgtggg
    15361 gacaggggtg tggggacagg ggtgtgggga caggggtctg gggacagggg tgtggggaca
    15421 ggggtcctgg ggacaggggt gtggggatag gggtgtgggg acaggggtgt ggggacaggg
    15481 gtgtggggac aggggtctgg ggacagcagc gcaaagagcc ccgccctgca gcctccagct
    15541 ctcctggtct aatgtggaaa gtggcccagg tgagggcttt gctctcctgg agacatttgc
    15601 ccccagctgt gagcagggac aggtctggcc accgggcccc tggttaagac tctaatgacc
    15661 cgctggtcct gaggaagagg tgctgacgac caaggagatc ttcccacaga cccagcacca
    15721 gggaaatggt ccggaaattg cagcctcagc ccccagccat ctgccgaccc ccccacccca
    15781 ggccctaatg ggccaggcgg caggggttga gaggtagggg agatgggctc tgagactata
    15841 aagccagcgg gggcccagca gccctcagcc ctccaggaca ggctgcatca gaagaggcca
    15901 tcaagcaggt ctgttccaag ggcctttgcg tcaggtgggc tcaggattcc agggtggctg
    15961 gaccccaggc cccagctctg cagcagggag gacgtggctg ggctcgtgaa gcatgtgggg
    16021 gtgagcccag gggccccaag gcagggcacc tggccttcag cctgcctcag ccctgcctgt
    16081 ctcccagatc actgtccttc tgccatggcc ctgtggatgc gcctcctgcc cctgctggcg
    16141 ctgctggccc tctggggacc tgacccagcc gcagcctttg tgaaccaaca cctgtgcggc
    16201 tcacacctgg tggaagctct ctacctagtg tgcggggaac gaggcttctt ctacacaccc
    16261 aagacccgcc gggaggcaga ggacctgcag ggtgagccaa ctgcccattg ctgcccctgg
    16321 ccgcccccag ccaccccctg ctcctggcgc tcccacccag catgggcaga agggggcagg
    16381 aggctgccac ccagcagggg gtcaggtgca cttttttaaa aagaagttct cttggtcacg
    16441 tcctaaaagt gaccagctcc ctgtggccca gtcagaatct cagcctgagg acggtgttgg
    16501 cttcggcagc cccgagatac atcagagggt gggcacgctc ctccctccac tcgcccctca
    16561 aacaaatgcc ccgcagccca tttctccacc ctcatttgat gaccgcagat tcaagtgttt
    16621 tgttaagtaa agtcctgggt gacctggggt cacagggtgc cccacgctgc ctgcctctgg
    16681 gcgaacaccc catcacgccc ggaggagggc gtggctgcct gcctgagtgg gccagacccc
    16741 tgtcgccagg cctcacggca gctccatagt caggagatgg ggaagatgct ggggacaggc
    16801 cctggggaga agtactggga tcacctgttc aggctcccac tgtgacgctg ccccggggcg
    16861 ggggaaggag gtgggacatg tgggcgttgg ggcctgtagg tccacaccca gtgtgggtga
    16921 ccctccctct aacctgggtc cagcccggct ggagatgggt gggagtgcga cctagggctg
    16981 gcgggcaggc gggcactgtg tctccctgac tgtgtcctcc tgtgtccctc tgcctcgccg
    17041 ctgttccgga acctgctctg cgcggcacgt cctggcagtg gggcaggtgg agctgggcgg
    17101 gggccctggt gcaggcagcc tgcagccctt ggccctggag gggtccctgc agaagcgtgg
    17161 cattgtggaa caatgctgta ccagcatctg ctccctctac cagctggaga actactgcaa
    17221 cggtacctac ccatacgatg ttccagatta cgctggcagt ggagagggca gaggaagtct
    17281 gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg gaagacgcca aaaacataaa
    17341 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
    17401 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
    17461 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
    17521 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
    17581 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
    17641 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
    17701 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
    17761 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgctcgt
    17821 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
    17881 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
    17941 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
    18001 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
    18061 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
    18121 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
    18181 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
    18241 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
    18301 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
    18361 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
    18421 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
    18481 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
    18541 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
    18601 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
    18661 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
    18721 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
    18781 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
    18841 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
    18901 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
    18961 cgccgtggtc gacgagggca gaggcagcct gctgacatgt ggcgacgtgg aagagaaccc
    19021 tggccccatg catatgccac ctcctcgcct cctcttcttc ctcctcttcc tcacccccat
    19081 ggaagtcagg cccgaggaac ctctagtggt gaaggtggaa gagggagata acgctgtgct
    19141 gcagtgcctc aaggggacct cagatggccc cactcagcag ctgacctggt ctcgggagtc
    19201 cccgcttaaa cccttcttaa aactcagcct ggggctgcca ggcctgggaa tccacatgag
    19261 gcccctggcc atctggcttt tcatcttcaa cgtctctcaa cagatggggg gcttctacct
    19321 gtgccagccg gggcccccct ctgagaaggc ctggcagcct ggctggacag tcaatgtgga
    19381 gggcagcggg gagctgttcc ggtggaatgt ttcggaccta ggtggcctgg gctgtggcct
    19441 gaagaacagg tcctcagagg gccccagctc cccttccggg aagctcatga gccccaagct
    19501 gtatgtgtgg gccaaagacc gccctgagat ctgggaggga gagcctccgt gtgtcccacc
    19561 gagggacagc ctgaaccaga gcctcagcca ggacctcacc atggcccctg gctccacact
    19621 ctggctgtcc tgtggggtac cccctgactc tgtgtccagg ggccccctct cctggaccca
    19681 tgtgcacccc aaggggccta agtcattgct gagcctagag ctgaaggacg atcgcccggc
    19741 cagagatatg tgggtaatgg agacgggtct gttgttgccc cgggccacag ctcaagacgc
    19801 tggaaagtat tattgtcacc gtggcaacct gaccatgtca ttccacctgg agatcactgc
    19861 tcggccagta ctatggcact ggctgctgag gactggtggc tggaaggtct cagctgtgac
    19921 tttggcttat ctgatcttct gcctgtgttc ccttgtgggc attcttcatc ttcaaagagc
    19981 cctggtcctg aggaggaaaa gaaagcgaat gactgacccc accaggagat tcttcaaagt
    20041 gacgcctccc ccaggaagcg ggccccagaa ccagtacggg aacgtgctgt ctctccccac
    20101 acccacctca ggcctcggac gcgcccagcg ttgggccgca ggcctggggg gcactgcccc
    20161 gtcttatgga aacccgagca gcgacgtcca ggcggatgga gccttggggt cccggagccc
    20221 gccgggagtg ggcccagaag aagaggaagg ggagggctat gaggaacctg acagtgagga
    20281 ggactccgag ttctatgaga acgactccaa ccttgggcag gaccagctct cccaggatgg
    20341 cagcggctac gagaaccctg aggatgagcc cctgggtcct gaggatgaag actccttctc
    20401 caacgctgag tcttatgaga acgaggatga agagctgacc cagccggtcg ccaggacaat
    20461 ggacttcctg agccctcatg ggtcagcctg ggaccccagc cgggaagcaa cctccctggc
    20521 agggtcccag tcctatgagg atatgagagg aatcctgtat gcagcccccc agctccgctc
    20581 cattcggggc cagcctggac ccaatcatga ggaagatgca gactcttatg agaacatgga
    20641 taatcccgat gggccagacc cagcctgggg aggagggggc cgcatgggca cctggagcac
    20701 caggggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
    20761 tggcccagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
    20821 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
    20881 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
    20941 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
    21001 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
    21061 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
    21121 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
    21181 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
    21241 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
    21301 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
    21361 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
    21421 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
    21481 gtaattaatt aa
//
