LOCUS       sequence-307894-        9345 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of FLAG/HA-tagged HDLBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Zinnall U, Milek M, Minia I, Vieira-Vieira CH, Muller S, Mastrobuoni
            G, Hazapis OG, Del Giudice S, Schwefel D, Bley N, Voigt F, Chao JA, 
            Kempa S, Huttelmaier S, Selbach M, Landthaler M
  TITLE     HDLBP binds ER-targeted mRNAs by multivalent interactions to promote
            protein synthesis of transmembrane and secreted proteins.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2727. doi: 10.1038/s41467-022-30322-7.
  PUBMED    35585045
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2727. doi"; pages: " 
            10.1038/s41467-022-30322-7"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-HDLBP
FEATURES             Location/Qualifiers
     source          1..9345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             379..402
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             406..429
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             430..456
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    472..496
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4310..4334)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4387..4428
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(4485..4509)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4562..4603
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4683..4700)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4689..4913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5046..5065)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5197..5244
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5252..6274
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5252..6274
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6404..6525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6441..6460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6495..6514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6574..6590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6574..6590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6587..6609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6598..6614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6622..6628,6629..6646,6647..6652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6667..6688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6805..6822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6976..7564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7056..7075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7735..8526,8527..8595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8358..8377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8596..8700)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8775..8794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8966..9345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccatgca ccaccaccac caccaccacc acatggacta caaggacgac
      421 gatgacaagt acccttatga cgtgcccgat tacgctttga accagatatc aacaagtttg
      481 tacaaaaaag caggctccac catgagttcc gttgcagttt tgacccaaga gagttttgct
      541 gaacaccgaa gtgggctggt tccgcaacaa atcaaagttg ccactctaaa ttcagaagag
      601 gagagcgacc ctccaaccta caaggatgcc ttccctccac ttcctgagaa agctgcttgc
      661 ctggaaagtg cccaggaacc cgctggagcc tgggggaaca agatccgacc catcaaggct
      721 tctgtcatca ctcaggtgtt ccatgtaccc ctggaggaga gaaaatacaa ggatatgaac
      781 cagtttggag aaggtgaaca agcaaaaatc tgccttgaga tcatgcagag aactggtgct
      841 cacttggagc tgtctttggc caaagaccaa ggcctctcca tcatggtgtc aggaaagctg
      901 gatgctgtca tgaaagctcg gaaggacatt gttgctagac tgcagactca ggcctcagca
      961 actgttgcca ttcccaaaga acaccatcgc tttgttattg gcaaaaatgg agagaaactg
     1021 caagacttgg agctaaaaac tgcaaccaaa atccagatcc cacgcccaga tgaccccagc
     1081 aatcagatca agatcactgg caccaaagag ggcatcgaga aagctcgcca tgaagtctta
     1141 ctcatctctg ccgagcagga caaacgtgct gtggagaggc tagaagtaga aaaggcattc
     1201 caccccttca tcgctgggcc gtataataga ctggttggcg agatcatgca ggagacaggc
     1261 acgcgcatca acatcccccc acccagcgtg aaccggacag agattgtctt cactggagag
     1321 aaggaacagt tggctcaggc tgtggctcgc atcaagaaga tttatgagga gaagaaaaag
     1381 aagactacaa ccattgcagt ggaagtgaag aaatcccaac acaagtatgt cattgggccc
     1441 aagggcaatt cattgcagga gatccttgag agaactggag tttccgttga gatcccaccc
     1501 tcagacagca tctctgagac tgtaatactt cgaggcgaac ctgaaaagtt aggtcaggcg
     1561 ttgactgaag tctatgccaa ggccaatagc ttcaccgtct cctctgtcgc cgccccttcc
     1621 tggcttcacc gtttcatcat tggcaagaaa gggcagaacc tggccaaaat cactcagcag
     1681 atgccaaagg ttcacatcga gttcacagag ggcgaagaca agatcaccct ggagggccct
     1741 acagaggatg tcaatgtggc ccaggaacag atagaaggca tggtcaaaga tttgattaac
     1801 cggatggact atgtggagat caacatcgac cacaagttcc acaggcacct cattgggaag
     1861 agcggtgcca acataaacag aatcaaagac cagtacaagg tgtccgtgcg catccctcct
     1921 gacagtgaga agagcaattt gatccgcatc gagggggacc cacagggcgt gcagcaggcc
     1981 aagcgagagc tgctggagct tgcatctcgc atggaaaatg agcgtaccaa ggatctaatc
     2041 attgagcaaa gatttcatcg cacaatcatt gggcagaagg gtgaacggat ccgtgaaatt
     2101 cgtgacaaat tcccagaggt catcattaac tttccagacc cagcacaaaa aagtgacatt
     2161 gtccagctca gaggacctaa gaatgaggtg gaaaaatgca caaaatacat gcagaagatg
     2221 gtggcagatc tggtggaaaa tagctattca atttctgttc cgatcttcaa acagtttcac
     2281 aagaatatca ttgggaaagg aggcgcaaac attaaaaaga ttcgtgaaga aagcaacacc
     2341 aaaatcgacc ttccagcaga gaatagcaat tcagagacca ttatcatcac aggcaagcga
     2401 gccaactgcg aagctgcccg gagcaggatt ctgtctattc agaaagacct ggccaacata
     2461 gccgaggtag aggtctccat ccctgccaag ctgcacaact ccctcattgg caccaagggc
     2521 cgtctgatcc gctccatcat ggaggagtgc ggcggggtcc acattcactt tcccgtggaa
     2581 ggttcaggaa gcgacaccgt tgttatcagg ggcccttcct cggatgtgga gaaggccaag
     2641 aagcagctcc tgcatctggc ggaggagaag caaaccaaga gtttcactgt tgacatccgc
     2701 gccaagccag aataccacaa attcctcatc ggcaaggggg gcggcaaaat tcgcaaggtg
     2761 cgcgacagca ctggagcacg tgtcatcttc cctgcggctg aggacaagga ccaggacctg
     2821 atcaccatca ttggaaagga ggacgccgtc cgagaggcac agaaggagct ggaggccttg
     2881 atccaaaacc tggataatgt ggtggaagac tccatgctgg tggaccccaa gcaccaccgc
     2941 cacttcgtca tccgcagagg ccaggtcttg cgggagattg ctgaagagta tggcggggtg
     3001 atggtcagct tcccacgctc tggcacacag agcgacaaag tcaccctcaa gggcgccaag
     3061 gactgtgtgg aggcagccaa gaaacgcatt caggagatca ttgaggacct ggaagctcag
     3121 gtgacattag aatgtgctat accccagaaa ttccatcgat ctgtcatggg ccccaaaggt
     3181 tccagaatcc agcagattac tcgggatttc agtgttcaaa ttaaattccc agacagagag
     3241 gagaacgcag ttcacagtac agagccagtt gtccaggaga atggggacga agctggggag
     3301 gggagagagg ctaaagattg tgaccccggc tctccaagga ggtgtgacat catcatcatc
     3361 tctggccgga aagaaaagtg tgaggctgcc aaggaagctc tggaggcatt ggttcctgtc
     3421 accattgaag tagaggtgcc ctttgacctt caccgttacg ttattgggca gaaaggaagt
     3481 gggatccgca agatgatgga tgagtttgag gtgaacatac atgtcccggc acctgagctg
     3541 cagtctgaca tcatcgccat cacgggcctc gctgcaaatt tggaccgggc caaggctgga
     3601 ctgctggagc gtgtgaagga gctacaggcc gagcaggagg accgggcttt aaggagtttt
     3661 aagctgagtg tcactgtaga ccccaaatac catcccaaga ttatcgggag aaagggggca
     3721 gtaattaccc aaatccggtt ggagcatgac gtgaacatcc agtttcctga taaggacgat
     3781 gggaaccagc cccaggacca aattaccatc acagggtacg aaaagaacac agaagctgcc
     3841 agggatgcta tactgagaat tgtgggtgaa cttgagcaga tggtttctga ggacgtcccg
     3901 ctggaccacc gcgttcacgc ccgcatcatt ggtgcccgcg gcaaagccat tcgcaaaatc
     3961 atggacgaat tcaaggtgga cattcgcttc ccacagagcg gagccccaga ccccaactgc
     4021 gtcactgtga cggggctccc agagaatgtg gaggaagcca tcgaccacat cctcaatctg
     4081 gaggaggaat acctagctga cgtggtggac agtgaggcgc tgcaggtata catgaaaccc
     4141 ccagcacacg aagaggccaa ggcaccttcc agaggctttg tggtgcggga cgcaccctgg
     4201 accgccagca gcagtgagaa ggctcctgac atgagcagct ctgaggaatt tcccagcttt
     4261 ggggctcagg tggctcccaa gaccctccct tggggcccca aacgatagga cccagctttc
     4321 ttgtacaaag tggttgatat ccagcacagt ggcggccgct caagtctaga gggcccgcgg
     4381 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg tacctgatcc
     4441 gcggccgcat agataactga tccagtgtgc ctcgagatat ctagacccag ctttcttgta
     4501 caaagtggtt gatatccagc acagtggcgg ccgctcaagt ctagagggcc cgcggttcga
     4561 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtacct gatccgcggc
     4621 cgcatagata actgatccag tgtgcctcga gtctagaggg cccgtttaaa cccgctgatc
     4681 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4741 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4801 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4861 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4921 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4981 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5041 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5101 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5161 caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta
     5221 ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt
     5281 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     5341 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     5401 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     5461 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     5521 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     5581 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     5641 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     5701 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     5761 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     5821 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     5881 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     5941 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     6001 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     6061 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     6121 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     6181 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     6241 acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac
     6301 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6361 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6541 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6661 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6721 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6781 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8701 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     8761 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8821 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     8881 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     8941 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     9001 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     9061 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     9121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
