LOCUS       sequence-308129-        9605 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Constitutive lentiviral expression of human CTSS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-CTSS-G418
FEATURES             Location/Qualifiers
     source          1..9605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2321..2345)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2353..2394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2445..3033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2498..2518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2916..2927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3105..3338
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3416..3537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3453..3472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3507..3526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3577..3712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3732..3751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3733..3751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3761..3778)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3761..3777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3770..3792)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3919..4374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4006..4025)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4216..4237
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4400..4504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4505..4573,4574..5365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4723..4742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5536..6124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6025..6044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6278..6295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6412..6433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6448..6453,6454..6471,6472..6478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6486..6502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6491..6513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6510..6526
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6510..6526
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6545..6565
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6547..6565
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6593..6819
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6820..7000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7047..7172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7665..7898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8083..8127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8358..8568
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8380..8576
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8427..8562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8604..9398
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8604..9398
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8658..8677)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9268..9287
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9446..9563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgaaac ggctggtttg tgtgctcttg gtgtgctcct ctgcagtggc acagttgcat
     1381 aaagatccta ccctggatca ccactggcat ctctggaaga aaacctatgg caaacaatac
     1441 aaggaaaaga atgaagaagc agtacgacgt ctcatctggg aaaagaatct aaagtttgtg
     1501 atgcttcaca acctggagca ttcaatggga atgcactcat acgatctggg catgaaccac
     1561 ctgggagaca tgaccagtga agaagtgatg tctttgatga gttccctgag agttcccagc
     1621 cagtggcaga gaaatatcac atataagtca aaccctaatc gaatattgcc tgattctgtg
     1681 gactggagag agaaagggtg tgttactgaa gtgaaatatc aaggttcttg tggtgcttgc
     1741 tgggctttca gtgctgtggg ggccctggaa gcacagctga agctgaaaac aggaaagctg
     1801 gtgtctctca gtgcccagaa cctggtggat tgctcaactg aaaaatatgg aaacaaaggc
     1861 tgcaatggtg gcttcatgac aacggctttc cagtacatca ttgataacaa gggcatcgac
     1921 tcagacgctt cctatcccta caaagccatg gatcagaaat gtcaatatga ctcaaaatat
     1981 cgtgctgcca catgttcaaa gtacactgaa cttccttatg gcagagaaga tgtcctgaaa
     2041 gaagctgtgg ccaataaagg cccagtgtct gttggtgtag atgcgcgtca tccttctttc
     2101 ttcctctaca gaagtggtgt ctactatgaa ccatcctgta ctcagaatgt gaatcatggt
     2161 gtacttgtgg ttggctatgg tgatcttaat gggaaagaat actggcttgt gaaaaacagc
     2221 tggggccaca actttggtga agaaggatat attcggatgg caagaaataa aggaaatcat
     2281 tgtgggattg ctagctttcc ctcttaccca gaaatctaga acccagcttt cttgtacaaa
     2341 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     2401 tgaactagta ccggttaagt cgacaatcaa cgcgttaagt cgacaatcaa cctctggatt
     2461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2821 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     2881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3001 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     3061 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3121 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3181 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3241 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3301 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     3361 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     3421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3541 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3601 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3661 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     3721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     3781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3901 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4261 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     4381 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4441 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4501 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4561 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4621 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4681 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4741 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4801 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4861 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4921 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4981 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5041 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5101 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5161 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5221 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5281 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5341 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5401 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5461 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5521 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5581 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5641 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5701 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5761 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5821 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5881 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5941 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6001 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6061 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6121 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6181 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6241 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6301 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6361 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6421 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6481 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6541 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     6601 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     6661 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     6721 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     6781 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     6841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     7021 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     7081 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     7141 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     7201 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     7261 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     7321 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     7381 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     7441 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     7501 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     7561 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     7621 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     7681 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     7741 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     7801 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7861 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7921 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7981 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     8041 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     8101 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     8161 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     8221 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     8281 ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa
     8341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa
     8401 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8461 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8521 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     8581 tttctagagg taccgtggcc accatgattg aacaagatgg attgcacgca ggttctccgg
     8641 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     8701 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     8761 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     8821 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     8881 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     8941 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     9001 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     9061 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     9121 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     9181 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     9241 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     9301 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     9361 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagt taacgaattc tagatcttga
     9421 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9601 gccgg
//
