LOCUS       sequence-308168-       10801 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Constitutive lentiviral expression of human FURIN..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-FURIN-puro
FEATURES             Location/Qualifiers
     source          1..10801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2621..2630
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3710..3734)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3742..3783
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3834..4422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3887..3907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4305..4316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4494..4727
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4805..4926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4842..4861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4896..4915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4966..5101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5121..5140)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5122..5140)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5150..5167)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5150..5166)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5159..5181)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5308..5763
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5395..5414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5605..5626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5789..5893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5894..5962,5963..6754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6112..6131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6925..7513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7414..7433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7667..7684
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7801..7822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7837..7842,7843..7860,7861..7867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7880..7902
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7899..7915
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7899..7915
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7934..7954
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7936..7954
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7982..8208
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8209..8389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8436..8561
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9054..9287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9472..9516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9747..9957
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9769..9965
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9816..9951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9997..10599
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9997..10016)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10493..10513
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10642..10759
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatggagc tgaggccctg gttgctatgg gtggtagcag caacaggaac cttggtcctg
     1381 ctagcagctg atgctcaggg ccagaaggtc ttcaccaaca cgtgggctgt gcgcatccct
     1441 ggaggcccag cggtggccaa cagtgtggca cggaagcatg ggttcctcaa cctgggccag
     1501 atcttcgggg actattacca cttctggcat cgaggagtga cgaagcggtc cctgtcgcct
     1561 caccgcccgc ggcacagccg gctgcagagg gagcctcaag tacagtggct ggaacagcag
     1621 gtggcaaagc gacggactaa acgggacgtg taccaggagc ccacagaccc caagtttcct
     1681 cagcagtggt acctgtctgg tgtcactcag cgggacctga atgtgaaggc ggcctgggcg
     1741 cagggctaca cagggcacgg cattgtggtc tccattctgg acgatggcat cgagaagaac
     1801 cacccggact tggcaggcaa ttatgatcct ggggccagtt ttgatgtcaa tgaccaggac
     1861 cctgaccccc agcctcggta cacacagatg aatgacaaca ggcacggcac acggtgtgcg
     1921 ggggaagtgg ctgcggtggc caacaacggt gtctgtggtg taggtgtggc ctacaacgcc
     1981 cgcattggag gggtgcgcat gctggatggc gaggtgacag atgcagtgga ggcacgctcg
     2041 ctgggcctga accccaacca catccacatc tacagtgcca gctggggccc cgaggatgac
     2101 ggcaagacag tggatgggcc agcccgcctc gccgaggagg ccttcttccg tggggttagc
     2161 cagggccgag gggggctggg ctccatcttt gtctgggcct cggggaacgg gggccgggaa
     2221 catgacagct gcaactgcga cggctacacc aacagtatct acacgctgtc catcagcagc
     2281 gccacgcagt ttggcaacgt gccgtggtac agcgaggcct gctcgtccac actggccacg
     2341 acctacagca gtggcaacca gaatgagaag cagatcgtga cgactgactt gcggcagaag
     2401 tgcacggagt ctcacacggg cacctcagcc tctgccccct tagcagccgg catcattgct
     2461 ctcaccctgg aggccaataa gaacctcaca tggcgggaca tgcaacacct ggtggtacag
     2521 acctcgaagc cagcccacct caatgccaac gactgggcca ccaatggtgt gggccggaaa
     2581 gtgagccact catatggcta cgggcttttg gacgcaggcg ccatggtggc cctggcccag
     2641 aattggacca cagtggcccc ccagcggaag tgcatcatcg acatcctcac cgagcccaaa
     2701 gacatcggga aacggctcga ggtgcggaag accgtgaccg cgtgcctggg cgagcccaac
     2761 cacatcactc ggctggagca cgctcaggcg cggctcaccc tgtcctataa tcgccgtggc
     2821 gacctggcca tccacctggt cagccccatg ggcacccgct ccaccctgct ggcagccagg
     2881 ccacatgact actccgcaga tgggtttaat gactgggcct tcatgacaac tcattcctgg
     2941 gatgaggatc cctctggcga gtgggtccta gagattgaaa acaccagcga agccaacaac
     3001 tatgggacgc tgaccaagtt caccctcgta ctctatggca ccgcccctga ggggctgccc
     3061 gtacctccag aaagcagtgg ctgcaagacc ctcacgtcca gtcaggcctg tgtggtgtgc
     3121 gaggaaggct tctccctgca ccagaagagc tgtgtccagc actgccctcc aggcttcgcc
     3181 ccccaagtcc tcgatacgca ctatagcacc gagaatgacg tggagaccat ccgggccagc
     3241 gtctgcgccc cctgccacgc ctcatgtgcc acatgccagg ggccggccct gacagattgc
     3301 ctcagctgcc ccagccacgc ctccttggac cctgtggagc agacttgctc ccggcaaagc
     3361 cagagcagcc gagagtcccc gccacagcag cagccacctc ggctgccccc ggaggtggag
     3421 gcggggcaac ggctgcgggc agggctgctg ccctcacacc tgcctgaggt ggtggccggc
     3481 ctcagctgcg ccttcatcgt gctggtcttc gtcactgtct tcctggtcct gcagctgcgc
     3541 tctggcttta gttttcgggg ggtgaaggtg tacaccatgg accgtggcct catctcctac
     3601 aaggggctgc cccctgaagc ctggcaggag gagtgcccgt ctgactcaga agaggacgag
     3661 ggccggggcg agaggaccgc ctttatcaaa gaccagagcg ccctctaaaa cccagctttc
     3721 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     3781 acgtagtaat gaactagtac cggttaagtc gacaatcaac gcgttaagtc gacaatcaac
     3841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3961 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4021 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4081 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4141 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4201 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4261 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4321 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4381 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     4441 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     4501 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     4561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     4741 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4801 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4921 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     4981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5041 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5101 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     5161 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5221 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5281 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     5341 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5401 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5461 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5521 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5581 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5641 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5701 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5761 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5821 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7681 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7741 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7801 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7861 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7921 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7981 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8041 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8101 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     8161 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     8221 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8281 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8341 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8401 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8461 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8521 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8581 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8641 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8701 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8761 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8821 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8881 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8941 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9001 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9061 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9121 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9181 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9241 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9301 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9361 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9421 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9481 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9541 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9601 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9661 cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca
     9721 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt
     9781 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9841 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9901 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9961 gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg
    10021 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
    10081 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
    10141 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
    10201 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
    10261 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
    10321 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    10381 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    10441 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
    10501 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    10561 cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca
    10621 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    10681 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    10741 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
    10801 g
//
