LOCUS       sequence-308173-       10910 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Constitutive lentiviral expression of human DPP4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-DPP4-G418
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3422..3430
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(3626..3650)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3658..3699
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3750..4338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3803..3823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4221..4232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4410..4643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4721..4842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4758..4777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4882..5017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5037..5056)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5038..5056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5066..5083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5066..5082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5075..5097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5224..5679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5311..5330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5521..5542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5705..5809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5810..5878,5879..6670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6028..6047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6841..7429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7330..7349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7583..7600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7717..7738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7753..7758,7759..7776,7777..7783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7791..7807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7796..7818
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7815..7831
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7815..7831
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7850..7870
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7852..7870
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7898..8124
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8125..8305
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8352..8477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8970..9203
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9388..9432
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9663..9873
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9685..9881
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9732..9867
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9909..10703
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9909..10703
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9963..9982)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10573..10592
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    10751..10868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgaaga caccgtggaa ggttcttctg ggactgctgg gtgctgctgc gcttgtcacc
     1381 atcatcaccg tgcccgtggt tctgctgaac aaaggcacag atgatgctac agctgacagt
     1441 cgcaaaactt acactctaac tgattactta aaaaatactt atagactgaa gttatactcc
     1501 ttaagatgga tttcagatca tgaatatctc tacaaacaag aaaataatat cttggtattc
     1561 aatgctgaat atggaaacag ctcagttttc ttggagaaca gtacatttga tgagtttgga
     1621 cattctatca atgattattc aatatctcct gatgggcagt ttattctctt agaatacaac
     1681 tacgtgaagc aatggaggca ttcctacaca gcttcatatg acatttatga tttaaataaa
     1741 aggcagctga ttacagaaga gaggattcca aacaacacac agtgggtcac atggtcacca
     1801 gtgggtcata aattggcata tgtttggaac aatgacattt atgttaaaat tgaaccaaat
     1861 ttaccaagtt acagaatcac atggacgggg aaagaagata taatatataa tggaataact
     1921 gactgggttt atgaagagga agtcttcagt gcctactctg ctctgtggtg gtctccaaac
     1981 ggcacttttt tagcatatgc ccaatttaac gacacagaag tcccacttat tgaatactcc
     2041 ttctactctg atgagtcact gcagtaccca aagactgtac gggttccata tccaaaggca
     2101 ggagctgtga atccaactgt aaagttcttt gttgtaaata cagactctct cagctcagtc
     2161 accaatgcaa cttccataca aatcactgct cctgcttcta tgttgatagg ggatcactac
     2221 ttgtgtgatg tgacatgggc aacacaagaa agaatttctt tgcagtggct caggaggatt
     2281 cagaactatt cggtcatgga tatttgtgac tatgatgaat ccagtggaag atggaactgc
     2341 ttagtggcac ggcaacacat tgaaatgagt actactggct gggttggaag atttaggcct
     2401 tcagaacctc attttaccct tgatggtaat agcttctaca agatcatcag caatgaagaa
     2461 ggttacagac acatttgcta tttccaaata gataaaaaag actgcacatt tattacaaaa
     2521 ggcacctggg aagtcatcgg gatagaagct ctaaccagtg attatctata ctacattagt
     2581 aatgaatata aaggaatgcc aggaggaagg aatctttata aaatccaact tagtgactat
     2641 acaaaagtga catgcctcag ttgtgagctg aatccggaaa ggtgtcagta ctattctgtg
     2701 tcattcagta aagaggcgaa gtattatcag ctgagatgtt ccggtcctgg tctgcccctc
     2761 tatactctac acagcagcgt gaatgataaa gggctgagag tcctggaaga caattcagct
     2821 ttggataaaa tgctgcagaa tgtccagatg ccctccaaaa aactggactt cattattttg
     2881 aatgaaacaa aattttggta tcagatgatc ttgcctcctc attttgataa atccaagaaa
     2941 tatcctctac tattagatgt gtatgcaggc ccatgtagtc aaaaagcaga cactgtcttc
     3001 agactgaact gggccactta ccttgcaagc acagaaaaca ttatagtagc tagctttgat
     3061 ggcagaggaa gtggttacca aggagataag atcatgcatg caatcaacag aagactggga
     3121 acatttgaag ttgaagatca aattgaagca gccagacaat tttcaaaaat gggatttgtg
     3181 gacaacaaac gaattgcaat ttggggctgg tcatatggag ggtacgtaac ctcaatggtc
     3241 ctgggatcgg gaagtggcgt gttcaagtgt ggaatagccg tggcgcctgt atcccggtgg
     3301 gagtactatg actcagtgta cacagaacgt tacatgggtc tcccaactcc agaagacaac
     3361 cttgaccatt acagaaattc aacagtcatg agcagagctg aaaattttaa acaagttgag
     3421 tacctcctta ttcatggaac agcagatgat aacgttcact ttcagcagtc agctcagatc
     3481 tccaaagccc tggtcgatgt tggagtggat ttccaggcaa tgtggtatac tgatgaagac
     3541 catggaatag ctagcagcac agcacaccaa catatatata cccacatgag ccacttcata
     3601 aaacaatgtt tctctttacc ttagaaccca gctttcttgt acaaagtggt tgatatcggt
     3661 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtaccggt
     3721 taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     3781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4141 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4321 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     4381 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4441 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4621 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     4681 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     4741 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4801 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     4861 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4981 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     5041 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5101 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5161 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5221 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5401 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5641 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     5701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7501 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7561 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7621 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7681 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7741 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7801 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7861 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7921 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7981 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     8041 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     8101 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     8161 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8221 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8281 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8341 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8401 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8461 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8521 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8581 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8641 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8701 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8761 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8821 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8881 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8941 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     9001 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     9061 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     9121 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     9181 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     9241 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9301 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9361 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9421 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9481 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9541 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9601 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9661 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     9721 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9781 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     9841 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtaccg
     9901 tggccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     9961 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
    10021 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
    10081 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
    10141 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
    10201 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
    10261 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
    10321 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    10381 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
    10441 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    10501 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
    10561 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    10621 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    10681 gccttcttga cgagttcttc tgagttaacg aattctagat cttgagacaa atggcagtat
    10741 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10801 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    10861 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
//
