LOCUS       sequence-308886-        3869 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1947
FEATURES             Location/Qualifiers
     source          1..3869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(157..184)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(276..362)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     411..433
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     425..442
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     426..442
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             771..791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1655..1978
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(1999..2018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2000..2018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2023..2039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2023..2039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2141..2160)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2152..2961
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2219..2238)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3108..3696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3597..3616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3850..3867
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
      121 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
      181 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
      241 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
      301 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
      361 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
      421 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgatcg attttttgca
      481 aattacgagc gttgtagggg gcggagcgat aggtcctata ggttttggta tatcatcatt
      541 cattcattca ttggtacatt catttaccca ccttcctctt tctgagcttc tctggagttc
      601 tgtgcttcct ttttccctta tctttatact gtaattttta actttcaggc attgattgga
      661 tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
      721 acattttcag gaggaccctt gcttggaggg taccgagctc agaaaaaatg ccaaagaaga
      781 agcgtaaggt cctgcagatg gtctccaagg gagaggctgt catcaaggag ttcatgcgtt
      841 tcaaggtcca catggaggga tccatgaacg gacacgagtt cgagatcgag ggagagggag
      901 agggacgtcc atacgaggga acccaaaccg ccaagctcaa ggtcaccaag ggaggtaagt
      961 ttgtgataat ccaatttcaa ttcgcaatgg tcatcgtttt ttcaggacca ctcccattct
     1021 cctgggacat cctctcccca caattcatgt acggatcccg tgccttcatc aagcacccag
     1081 ccgacatccc agactactac aagcaatcct tcccagaggg attcaagtgg gagcgtgtga
     1141 tgaacttcga ggtaagtttt tactccgctt ttaacaatgg ttgtttgaca tcattttttc
     1201 aggacggagg agccgtcacc gtcacccaag acacctccct cgaggacgga accctcatct
     1261 acaaggtcaa gctccgtgga accaacttcc caccagacgg accagtcatg caaaagaaga
     1321 ccatgggatg ggaggcctcc accgagcgtc tctacccaga ggacggagtc ctcaaaggta
     1381 agtttgatga aacggtttcg tcttatatac actaatggta cttttcagga gacatcaaga
     1441 tggccctccg tctcaaggac ggaggacgtt acctcgccga cttcaagacc acctacaagg
     1501 ccaagaagcc agtccaaatg ccaggagcct acaacgtcga ccgtaagctc gacatcacct
     1561 cccacaacga ggactacacc gtcgtcgagc aatacgagcg ttccgaggga cgtcactcca
     1621 ccggaggaat ggacgagctc tacaagtaag ataaatgcaa aatcctttca agcattccct
     1681 tcttctctat cactcttctt tctttttgtc aaaaaattct ctcgctaatt tatttgcttt
     1741 tttaatgtta ttattttatg actttttata gtcactgaaa agtttgcatc tgagtgaagt
     1801 gaatgctatc aaaatgtgat tctgtctgat gtactttcac aatctctctt caattccatt
     1861 ttgaagtgct ttaaacccga aaggttgaga aaaatgcgag cgctcaaata tttgtattgt
     1921 gttcgttgag tgacccaaca aaaagaggaa actttattgt gccgccaaga aaaaagtctc
     1981 agccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     2041 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     2101 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     2161 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2221 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2281 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2341 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2401 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2461 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2521 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2581 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2641 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2701 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2761 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2821 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2881 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2941 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     3001 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     3061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3841 accgagcgca gcgagtcagt gagcgagga
//
