LOCUS       sequence-308988-        4699 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Can be used to generate AAV virus that will express DreO recombinase
            in neurons from the synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV phSyn1(S)-DreO-bGHpA
FEATURES             Location/Qualifiers
     source          1..4699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      211..666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(298..317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     508..529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(683..702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     802..824
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(862..880)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        948..1052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1053..1121,1122..1913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1271..1290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2084..2672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2573..2592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2734..2863
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2899..3346
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3393..3413
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4459..4476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4465..4672
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   join(4695..4699,1..136)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(4695..4699,1..125)
                     /label=AAV2 ITR
ORIGIN
        1 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
       61 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
      121 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      181 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
      241 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
      301 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      361 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      421 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      481 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      541 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
      601 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
      661 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      721 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      781 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      841 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      901 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2701 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
     2761 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     2821 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtctg
     2881 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2941 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     3001 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     3061 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     3121 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     3181 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     3241 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     3301 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagacgc gtggacggta
     3361 cccttcaggt cgagaaatcg attgccacca tgcctaagaa gaagaggaag gtttctgagc
     3421 tgattattag tggttcatct ggtggattcc tgcgaaacat cggcaaagag tatcaggagg
     3481 ccgctgaaaa cttcatgagg tttatgaatg accagggggc gtacgctcct aacactttga
     3541 gggatttgag gttggtcttt catagctggg ccagatggtg ccatgctcgg cagcttgcat
     3601 ggtttccaat tagtcctgaa atggcacgcg aatactttct tcagttgcac gatgcagacc
     3661 tggcctccac taccatcgac aagcactatg ctatgcttaa tatgcttctg tcccactgcg
     3721 gactgccacc cttgtccgac gacaagtcag tgagtcttgc catgagaaga attagaagag
     3781 aagccgcaac cgaaaagggt gagaggacag gacaggcaat ccccctgcgc tgggacgacc
     3841 tgaagctgct ggatgtgctg ctcagcagga gcgagcggct ggtcgacctg cgcaacaggg
     3901 ctttcctgtt cgtagcctat aacaccctca tgagaatgtc tgaaatatca cgcatcaggg
     3961 ttggggactt ggatcagaca ggagacacag tgaccctgca catcagtcac actaagacaa
     4021 tcaccacagc tgcgggcctt gacaaagtgc tctcccggcg aaccacagca gtgctcaatg
     4081 actggctgga cgtcagtggg cttagagaac atccagacgc tgtgctcttc ccacctatac
     4141 accggtcaaa caaagcccgc attactacca cgcccctgac cgcccctgcc atggagaaga
     4201 ttttcagtga tgcctgggtg ctgctgaaca aacgggacgc cacccccaat aaagggaggt
     4261 ataggacctg gaccggccat tccgccaggg tgggtgccgc aatagacatg gccgagaaac
     4321 aggtgtctat ggtcgagatt atgcaggaag ggacatggaa gaagcctgaa acactgatgc
     4381 ggtatctcag aaggggcgga gtgtccgtgg gagccaattc tcgactgatg gatagctaag
     4441 gatccgctag cagatctgcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4501 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4561 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4621 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gacacgtgcg
     4681 gaccgagcgg ccgcaggaa
//
