LOCUS       sequence-309643-       12190 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Betapudi V
  TITLE     Myosin II motor proteins with different functions determine the fate
            of lamellipodia extension during cell spreading.
  JOURNAL   PLoS One. 2010 Jan 5;5(1):e8560.
  PUBMED    20052411
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-01-5"; volume: "5(1)"; pages: "e8560"
COMMENT     Sequence Label: pCMV-GFP-MHC-IIB
FEATURES             Location/Qualifiers
     source          1..12190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8751..8767)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8751..8767)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    8918..9039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8955..8974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9009..9028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9046..9501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9183..9204)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9395..9414
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9528..9632
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9634..9991
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9634..9825
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9657..9677)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9842..9977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10026..10820
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10026..10820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10080..10099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10690..10709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11008..11027)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11052..11099
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11428..12016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11917..11936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt actcagatct cgagctcaag cttcgaattc caatggcgca gagaactgga
     1321 ctcgaggatc cagagaggta tctctttgtg gacagggctg tcatctacaa ccctgccact
     1381 caagctgatt ggacagctaa aaagctagtg tggattccat cagaacgcca tggttttgag
     1441 gcagctagta tcaaagaaga acggggagat gaagttatgg tggagttggc agagaatgga
     1501 aagaaagcaa tggtcaacaa agatgatatt cagaagatga acccacctaa gttttccaag
     1561 gtggaggata tggcagaatt gacatgcttg aatgaagctt ccgttttaca taatctgaag
     1621 gatcgctact attcaggact aatctatact tattctggac tcttctgtgt agttataaac
     1681 ccttacaaga atcttccaat ttactctgag aatattattg aaatgtacag agggaagaag
     1741 cgtcatgaga tgcctccaca catctatgct atatctgaat ctgcttacag atgcatgctt
     1801 caagatcgtg aggaccagtc aattctttgc acgggtgagt caggtgctgg gaagacagaa
     1861 aatacaaaga aagttattca gtaccttgcc catgttgctt cttcacataa aggaagaaag
     1921 gaccataata ttcctgggga acttgaacgg cagcttttgc aagcaaatcc aattctggaa
     1981 tcatttggaa atgcgaagac tgtgaaaaat gataactcat ctcgttttgg caaatttatt
     2041 cggatcaact ttgatgtaac tggctatatc gttggggcca acattgaaac ataccttctg
     2101 gaaaagtctc gtgctgttcg tcaagcaaaa gatgaacgta cttttcatat cttttaccag
     2161 ttgttatctg gagcaggaga acacctaaag tctgatttgc ttcttgaagg atttaataac
     2221 tacaggtttc tctccaatgg ctatattcct attccgggac agcaagacaa agataatttc
     2281 caggagacca tggaagcaat gcacataatg ggcttctccc atgaagagat tctgtcaatg
     2341 cttaaagtag tatcttcagt gctacagttt ggaaatattt ctttcaaaaa ggagagaaat
     2401 actgatcaag cttccatgcc agaaaataca gttgcgcaga agctctgcca tcttcttggg
     2461 atgaatgtga tggagtttac tcgggccatc ctgactcccc ggatcaaggt cggccgagac
     2521 tatgtgcaaa aagcccagac caaagaacag gcagattttg cagtagaagc attggcaaaa
     2581 gctacctatg agcggctctt tcgctggctc gttcatcgca tcaataaagc tctggatagg
     2641 accaaacgtc agggagcatc tttcattgga atcctggata ttgctggatt tgaaattttt
     2701 gagctgaact cctttgaaca actttgcatc aactacacca atgagaagct gcagcagctg
     2761 ttcaaccaca ccatgtttat cctagaacaa gaggaatacc agcgcgaagg catcgagtgg
     2821 aacttcatcg atttcgggct ggatctgcag ccatgcatcg acctaataga gagacctgcg
     2881 aaccctcctg gtgtactggc ccttttggat gaagaatgct ggttccctaa agccacagat
     2941 aaaacctttg ttgaaaaact ggttcaagag caaggttccc actccaagtt tcagaaacct
     3001 cgacaattaa aagacaaagc tgatttttgc attatacatt atgcagggaa ggtggactat
     3061 aaggcagatg agtggctgat gaagaatatg gaccccctga atgacaacgt ggccaccctt
     3121 ttgcaccagt catcagacag atttgtggca gagctttgga aagatgtgga ccgtatcgtg
     3181 ggtctggatc aagtcactgg tatgactgag acagcttttg gctccgcata taaaaccaag
     3241 aagggcatgt ttcgtaccgt tgggcaactc tacaaagaat ctctcaccaa gctgatggca
     3301 actctccgaa acaccaaccc taactttgtt cgttgtatca ttccaaatca cgagaagagg
     3361 gctggaaaat tggatccaca cctagtccta gatcagcttc gctgtaatgg tgtcctggaa
     3421 gggatccgaa tctgtcgcca gggcttccct aaccgaatag ttttccagga attcagacag
     3481 agatatgaga tcctaactcc aaatgctatt cctaaaggtt ttatggatgg taaacaggcc
     3541 tgtgaacgaa tgatccgggc tttagaattg gacccaaact tgtacagaat tggacagagc
     3601 aagatatttt tcagagctgg agttctggca cacttagagg aagaaagaga tttaaaaatc
     3661 accgatatca ttatcttctt ccaggccgtt tgcagaggtt acctggccag aaaggccttt
     3721 gccaagaagc agcagcaact aagtgcctta aaggtcttgc agcggaactg tgccgcgtac
     3781 ctgaaattac ggcactggca gtggtggcga gtcttcacaa aggtgaagcc gcttctacaa
     3841 gtgactcgcc aggaggaaga acttcaggcc aaagatgaag agctgttgaa ggtgaaggag
     3901 aagcagacaa aggtggaagg agagctggag gagatggagc ggaagcacca gcagctttta
     3961 gaagagaaga atatccttgc agaacaacta caagcagaga ctgagctctt tgctgaagca
     4021 gaagagatga gggcaagact tgctgctaaa aagcaggaat tagaagagat tctacatgac
     4081 ttggagtcta gggttgaaga agaagaagaa agaaaccaaa tcctccaaaa tgaaaagaaa
     4141 aaaatgcaag cacatattca ggacctggaa gaacagctag acgaggagga aggggctcgg
     4201 caaaagctgc agctggaaaa ggtgacagca gaggccaaga tcaagaagat ggaagaggag
     4261 attctgcttc tcgaggacca aaattccaag ttcatcaaag aaaagaaact catggaagat
     4321 cgcattgctg agtgttcctc tcagctggct gaagaggaag aaaaggcgaa aaacttggcc
     4381 aaaatcagga ataagcaaga agtgatgatc tcagatctag aagaacgctt aaagaaggaa
     4441 gaaaagactc gtcaggaact ggaaaaggcc aaaagaaaac tcgacgggga gacgaccgac
     4501 ctgcaggacc agatcgcaga gctgcaggcg cagattgatg agctcaagct gcagctggcc
     4561 aagaaggagg aggagctgca gggcgcactg gccagaggtg atgatgaaac actccataag
     4621 aacaatgccc ttaaagttgt gcgagagcta caagcccaaa ttgctgaact tcaggaagac
     4681 tttgaatccg agaaggcttc acggaacaag gccgaaaagc agaaaaggga cttgagtgag
     4741 gaactggaag ctctgaaaac agagctggag gacacgctgg acaccacggc agcccagcag
     4801 gaactacgta caaaacgtga acaagaagtg gcagagctga agaaagctct tgaggaggaa
     4861 actaagaacc atgaagctca aatccaggac atgagacaaa gacacgcaac agccctggag
     4921 gagctctcag agcagctgga acaggccaag cggttcaaag caaatctaga gaagaacaag
     4981 cagggcctgg agacagataa caaggagctg gcgtgtgagg tgaaggtcct gcagcaggtc
     5041 aaggctgagt ctgagcacaa gaggaagaag ctcgacgcgc aggtccagga gctccatgcc
     5101 aaggtctctg aaggcgacag gctcagggtg gagctggcgg agaaagcaag taagctgcag
     5161 aatgagctag ataatgtctc cacccttctg gaagaagcag agaagaaggg tattaaattt
     5221 gctaaggatg cagctagtct tgagtctcaa ctacaggata cacaggagct tcttcaggag
     5281 gagacacgcc agaaactaaa cctgagcagt cggatccggc agctggaaga ggagaagaac
     5341 agtcttcagg agcagcagga ggaggaggag gaggccagga agaacctgga gaagcaagtg
     5401 ctggccctgc agtcccagtt ggctgatacc aagaagaaag tagatgacga cctgggaaca
     5461 attgaaagtc tggaagaagc caagaagaag cttctgaagg acgcggaggc cctgagccag
     5521 cgcctggagg agaaggcact ggcgtatgac aaactggaga agaccaagaa ccgcctgcag
     5581 caggagctgg acgacctcac ggtggacctg gaccaccagc gccaggtcgc ctccaacttg
     5641 gagaagaagc agaagaagtt tgaccagctg ttagcagaag agaagagcat ctctgctcgc
     5701 tatgccgaag agcgggaccg ggccgaagcc gaggccagag agaaagaaac caaagccctg
     5761 tcactggccc gggccctcga ggaagccctg gaggccaagg aggagtttga gaggcagaac
     5821 aagcagctcc gagcagacat ggaagacctc atgagctcca aagatgatgt gggaaaaaac
     5881 gttcacgaac ttgaaaaatc caaacgggcc ctagagcagc aggtggagga aatgaggacc
     5941 cagctggagg agctggaaga cgaactccag gccacggaag atgccaagct tcgtctggag
     6001 gtcaacatgc aggccatgaa ggcgcagttc gagagagacc tgcaaaccag ggatgagcag
     6061 aatgaagaga agaagcggct gctgatcaaa caggtgcggg agctcgaggc ggagctggag
     6121 gatgagagga aacagcgggc gcttgctgta gcttcaaaga aaaagatgga gatagacctg
     6181 aaggacctcg aagcccaaat cgaggctgcg aacaaagctc gggatgaggt gattaagcag
     6241 ctccgcaagc tccaggctca gatgaaggat taccaacgtg aattagaaga agctcgtgca
     6301 tccagagatg agatttttgc tcaatccaaa gagagtgaaa agaaattgaa gagtctggaa
     6361 gcagaaatcc ttcaattgca ggaggaactt gcctcatctg agcgagcccg ccgacacgcc
     6421 gagcaggaga gagatgagct ggcggacgag atcaccaaca gcgcctctgg caagtccgcg
     6481 ctgctggatg agaagcggcg tctggaagct cggatcgcac agctggagga ggagctggaa
     6541 gaggagcaga gcaacatgga gctgctcaac gaccgcttcc gcaagaccac tctacaggtg
     6601 gacacactga acgccgagct agcagccgag cgcagcgccg cccagaagag tgacaatgca
     6661 cgccagcaac tggagcggca gaacaaggag ctgaaggcca agctgcagga actcgagggt
     6721 gctgtcaagt ctaagttcaa ggccaccatc tcagccctgg aggccaagat tgggcagctg
     6781 gaggagcagc ttgagcagga agccaaggaa cgagcagccg ccaacaaatt agtccgtcgc
     6841 actgagaaga agctgaaaga aatcttcatg caggttgagg atgagcgtcg acacgcggac
     6901 cagtataaag agcagatgga gaaggccaac gctcggatga agcagcttaa acgccagctg
     6961 gaggaagcag aagaagaagc gacgcgtgcc aacgcatctc ggcgtaaact ccagcgggaa
     7021 ctggatgatg ccaccgaggc caacgagggc ctgagccgcg aggtcagcac cctgaagaac
     7081 cggctgaggc ggggtggccc catcagcttc tcttccagcc gatctggccg gcgccagctg
     7141 caccttgaag gagcttccct ggagctctcc gacgatgaca cagaaagtaa gaccagtgat
     7201 gtcaacgaga cgcagccacc ccagtcagag taaagttgca ggaagccaga ggaggcaata
     7261 cagtgggaca gttaggaatg cacccggggc ctcctgcaga tttcggaaat tggcaagcta
     7321 cgggattcct tcctgaaaga tcaactgtgt cttaaggctc tccagcctat gcatactgta
     7381 tcctgcttca gacttaggta caattgctcc cctttttata tatagacaca cacaggacac
     7441 atatattaaa cagattgttt catcattgca tctattttcc atatagtcat caagagacca
     7501 ttttataaaa catggtaaga ccctttttaa aacaaactcc aggcccttgg ttgcgggtcg
     7561 ctgggttatt ggggcagcgc cgtggtcgtc actcagtcgc tctgcatgct ctctgtcata
     7621 cagacaggta acctagttct gtgttcacgt ggcccccgac gactcagcca catcaagtct
     7681 cctagaccac tgtggactct aaactgcact tgtctctctc atttccttca aataatgatc
     7741 aatgctattt cagtgagcaa actgtgaaag gggctttgga aagagtagga ggggtgggct
     7801 ggatcggaag caacacccat ttggggttac catgtccatc ccccaagggg ggccctcccc
     7861 ctcgagtcga tggtgtcccg catctactca tgtgaactgg ccttggcgag ggctggtctg
     7921 tgcatagaag ggatagtggc cacactgcag ctgaggcccc aggtggcagc catggatcat
     7981 gtagacttcc agatggtctc ccgaaccgcc tggctctgcc ggcgccctcc tcacgtcagg
     8041 agcaagcagc cgtggacccc taagccgagc tggtggaagg cccctccccg tcgccagccg
     8101 ggccctcatg ctgaccttgc aaattcagcc gctgctttga gcccaaaatg ggaatattgg
     8161 ttttgtgtcc gaggcttgtt ccaagtttgt caatgaggtt tatggagcct ccagaacaga
     8221 tgccatcttc ctgaatgttg acatgccagt gggtgtgact ccttcatttt tccttctccc
     8281 ttccctttgg acagtgttac agtgaacact tagcatcctg tttttggttg gtagttaagc
     8341 aaactgacat tacggaaagt gccttagaca ctacagtact aagacaatgt tgaatatatc
     8401 attcgcctct ataacaattt aatgtattca gttttgactg tgcttcatat catgtacctc
     8461 tctagtcaaa gtggtattac agacattcag tgacaatgaa tcagtgttaa ttctaaatcc
     8521 ttgatcctct gcaatgtgct tgaaaacaca aaccttttgg gttaaaagct ttaacatcta
     8581 ttaggaagaa tttgtcctgt gggtttggaa tcttggattt tcccccttta tgaactgtac
     8641 tggctgttga ccaccagaca cctgaccgca aatatctttt cttgtattcc catatttcta
     8701 gacaatgatt tttgtaagac aatgcggccg cgaattcctg cagcccgggg gatccactag
     8761 ttctagagcg gccgccaccg cgggcccggg atccaccgga tctagataac tgatcataat
     8821 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8881 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     9061 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     9121 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     9181 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     9241 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     9301 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     9361 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     9421 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     9481 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     9541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     9601 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     9661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9901 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9961 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
    10021 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    10081 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    10141 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    10201 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    10261 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    10321 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    10381 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    10441 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    10501 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
    10561 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    10621 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    10681 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    10741 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    10801 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    10861 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10921 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10981 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
    11041 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
    11101 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
    11161 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
    11221 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
    11281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    11341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    11581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    11821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    11881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    11941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    12061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    12121 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    12181 tggagttccg
//
