LOCUS       sequence-309869-       10358 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-EBFP2-ccdB-G418
FEATURES             Location/Qualifiers
     source          1..10358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..325
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    90..108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    126..144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    162..180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     298..322
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(342..344,345..347,348..1058)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(387..408)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    1089..1213
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1238..1243,1244..1261,1262..1268)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1322..1981
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1388..1407)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2323..2628
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2507..2526
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2669..2793)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2798..2839
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2853..2870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2880..3390
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3309..3327
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3406..4197
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3406..4197
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3460..3479)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4070..4089
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             4216..4269
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4270..4887,4888..4893,4894..4929,4930..4932,4933..4968,
                     4969..4971,4972..5007,5008..5016)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5056..5076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5474..5485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5690..5923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5995..6116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6032..6051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6086..6105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6156..6291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6291..6362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6363..6431,6432..7223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6581..6600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7394..7982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7883..7902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8136..8153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8270..8291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8306..8311,8312..8329,8330..8336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8344..8360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8349..8371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8368..8384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8368..8384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8403..8423
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8405..8423
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8451..8677
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8678..8858
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8905..9030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9523..9756
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9941..9985
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10198..10315
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcgatatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
       61 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
      121 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
      181 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
      241 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      301 tcgtttagtg aaccgtcaga tcgcctggag aattggctag catggtgagc aagggcgagg
      361 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      421 agttcagcgt gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt
      481 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc
      541 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      601 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct
      661 acaagacccg cgccgaggtg aagttcgagg gcgacaccct agtgaaccgc atcgagctga
      721 agggcgtcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca
      781 acagccacaa catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca
      841 agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      901 cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg
      961 tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg
     1021 ccgccgggat cactctcggc atggacgagc tgtacaaggg ggccggccat atcagcggct
     1081 ctggttcgac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     1141 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     1201 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
     1261 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     1321 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1381 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1441 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1501 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1561 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1621 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1681 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1741 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1801 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1861 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1921 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1981 aagatctgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     2041 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     2101 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     2161 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     2221 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     2281 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     2341 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     2401 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     2461 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     2521 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2581 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2641 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     2701 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2761 cgtttctcgt tcagctttct tgtacaaagt ggttgcagga aagccaatcc caaacccact
     2821 cctaggtctc gacagtacag catagaccgg tccaccacca ccaccaccac taaggatccg
     2881 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     2941 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     3001 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     3061 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     3121 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     3181 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     3241 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     3301 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     3361 gttgaccgaa tcaccgacct ctctccccag cttaattaat tcaccatgat tgaacaagat
     3421 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3481 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3541 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3601 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3661 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3721 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3781 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3841 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3901 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3961 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4021 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4081 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4141 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttccct
     4201 gcaggtcccg gtgccgaagg tagaggttct ctcctcactt gtggtgatgt tgaagaaaac
     4261 cctggtccaa tgtctagact ggacaagagc aaagtcataa acggagctct ggaattactc
     4321 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     4381 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     4441 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     4501 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     4561 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     4621 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     4681 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     4741 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     4801 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     4861 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     4921 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     4981 cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg atccaagata
     5041 tcgtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5101 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5161 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5221 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5281 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5341 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     5401 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5461 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5521 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5581 cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag accaatgact
     5641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5701 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5761 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5821 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5881 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5941 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6121 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6181 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6241 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctttcaaata
     6301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6601 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6901 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8221 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8281 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8341 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8401 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8461 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     8521 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     8581 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     8641 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8701 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8761 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8881 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8941 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9001 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9061 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9121 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9181 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9241 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9301 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9361 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9421 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9481 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9541 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9601 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9661 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9721 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9781 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9841 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9901 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9961 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    10021 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10081 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10141 cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca tccacaattt
    10201 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    10261 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    10321 ttattacagg gacagcagag atccactttg gccgcgaa
//
