LOCUS       sequence-309917-       11231 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11231)
  TITLE     Immunoprecipitation of the endoplasmic reticulum (ER-IP)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ER-M-TAG (pGS555)
FEATURES             Location/Qualifiers
     source          1..11231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3353..4538
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3584..4529
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4486..4506
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4619..4628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5045..5737
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(5175..5193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5443..5462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5759..5839
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5849..6401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5993..6010)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6220..6239
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6410..6805
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6815..6871
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6881..7426
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             7436..7456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7463..8051
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7516..7536)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7934..7945)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8437..8670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8742..8863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8779..8798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8833..8852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8965..8984
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9249..9267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9335..9439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9440..9508,9509..10300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9658..9677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10471..11059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10960..10979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgagtt tactccctat
     2281 tctagacaaa tgcttgtcct tccttcctgt aacacagcca ttaaaccagg agcatcgccc
     2341 ttccccggcc ctcaggtaag aggaccaaat accgtagccg tttccaattt cagtccttta
     2401 gcgccacctg gtgctaacta ctctatcacg cttttatcca ataactacct ttgtaaattt
     2461 cctttcaaaa gttctggccg ggcgcggtgg ctcacgcttg taatcccagc actttgtgag
     2521 gggtcaggag ttcgagacat taatagggag gtggtccctg cagttacgcc aatgataacc
     2581 cccgccagaa aaatcttagt agccttccct ttttgttttc cgtgccccaa ctcggcggat
     2641 tgactcggcc ccttccggaa acacccgaat caacttctag tcaaattatt gttcacgccg
     2701 caatgaccca cccctggccc gcgtctgtgg aactgacccc tggtgtacag gagagttcgc
     2761 tgctgaaagt ggtcccaaag gggtactagt ttttaagctc ccaactcccc ctcccccagc
     2821 gtctggagga ttccacaccc tcgcaccgca ggggcgagga agtgggcgga gtccggtttt
     2881 ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc tgaggagact ccggtcactg
     2941 tcctcgcccc gcctccccct tccctcccct tggggaccac cgggcgccac gccgcgaacg
     3001 gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag ggccccaatt cccagcgggc
     3061 gcggcgcgcg gcccctcccc ccgccgggcg cgcgcccgct gccccgccct tcgtggccgc
     3121 ccggcgtggg cggtgccacc cctccccccg gcggccccgc gcgcagctcc cggctccctc
     3181 ccccttcgga tgtggcttga gctgtaggcg cggagtttaa ttaaaatcgc gtaccgtcga
     3241 cctcgacgga tcgggagatc tcgaccgagc tttgcaaaga tggataaagt tttaaacaga
     3301 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     3361 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     3421 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     3481 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     3541 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     3601 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     3661 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     3721 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     3781 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     3841 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     3901 tcttgtaaat gcgggccaag atgatctgca cactggtatt tcggtttttg gggccgcggg
     3961 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     4021 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     4081 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     4141 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     4201 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     4261 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     4321 ctcgatcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     4381 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     4441 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4501 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg accaagcttg
     4561 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattatc acaaggccgc
     4621 caccatggcg ccgtcgctgt ggaaggggct ggtgggcatc ggtctctttg ccctagccca
     4681 cgccgccttt tccgctgcgc agcatcgttc ttatatgcga ttaacagaaa aagaagatga
     4741 atcactgcca atagatatag ttcttcagac acttctggcc tttgcagtta cctgttacgg
     4801 tatagttcat attgcaggag agtttaaaga catggatgcc acttcagaac tgaaaaataa
     4861 gacatttgat acgttaagga atcacccatc cttttatgta tttaatcatc gtggtcgagt
     4921 acttttccgg ccttcggata cagcaaattc ttcaaaccaa gatgcattgt cctctaacac
     4981 atcattgaag ttacgaaaac tcgaatcact gcgtcgtggt ggtggttctg gaggcggcgg
     5041 atcagtgagc aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga
     5101 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5161 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga
     5221 catcctgtcc cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat
     5281 ccccgactac tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5341 cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat
     5401 ctacaaggtg aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa
     5461 gacaatgggc tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg
     5521 cgacattaag atggccctgc gcctgaagga cggcggccgc tacctggcgg acttcaagac
     5581 cacctacaag gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt
     5641 ggacatcacc tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg
     5701 ccgccactcc accggcggca tggacgagct gtacaagggc ggatcaggtg gtggttctta
     5761 cccatacgat gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata
     5821 tgatgtcccc gactatgcct aaaaactaac gttactggcc gaagccgctt ggaataaggc
     5881 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5941 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6001 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     6061 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     6121 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     6181 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     6241 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     6301 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     6361 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa tggccaagcc
     6421 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     6481 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     6541 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     6601 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     6661 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     6721 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     6781 gccctctggt tatgtgtggg agggcggatc cggcgcaaca aacttctctc tgctgaaaca
     6841 agccggagat gtcgaagaga atcctggacc gggatccggc atggacaaag actgcgaaat
     6901 gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt gcgaacaggg
     6961 cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc
     7021 tgccccagcc gccgtgctgg gcggaccaga gccactgatg caggccaccg cctggctcaa
     7081 cgcctacttt caccagcctg aggccatcga ggagttccct gtgccagccc tgcaccaccc
     7141 agtgttccag caggagagct ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa
     7201 gttcggagag gtcatcagct acagccacct ggccgccctg gccggcaatc ccgccgccac
     7261 cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc cctgccaccg
     7321 ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc gggctcgccg tgaaagagtg
     7381 gctgctggcc cacgagggcc acagactggg caagcctggg ctgggtggcg ggagcccaaa
     7441 gaagaagcgg aaggtgtagc ggaatcaacc tctggattac aaaatttgtg aaagattgac
     7501 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7561 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7621 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7681 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7741 aactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7801 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7861 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt
     7921 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7981 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8041 cgcctccccg catcgatacc gagcgctgct cgaggtacgc caacagagcg agattccgtc
     8101 tcaaagaaaa aaaaagtaat gaaatgaata aaatgagtcc tagagccagt aaatgtcgta
     8161 aatgtctcag ctagtcaggt agtaaaaggt ctcaactagg cagtggcaga gcaggattca
     8221 aattcagggc tgttgtgatg cctccgcaga ctctgagcgc cacctggtgg taatttgtct
     8281 gtgcctcttc tgacgtggaa gaacagcaac taacacacta acacggcatt tactatgggc
     8341 cagccattgt tctaatgtta actgtggtac ctttaagacc aatgacttac aaggcagctg
     8401 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8461 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8641 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     8701 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8761 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8821 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8881 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccggat cccttgagtg
     9001 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     9061 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     9121 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     9181 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta attcttgaag
     9241 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     9301 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9361 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9421 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9481 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9541 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9601 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9661 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     9721 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9781 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9841 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9901 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9961 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10021 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10081 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10141 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10201 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    10261 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    10321 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10381 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10441 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10501 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10561 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    10621 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10681 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10741 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10801 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10861 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10921 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10981 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11041 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    11101 ctggcctttt tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
    11161 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
    11221 gcctcgcgtc g
//
