LOCUS       sequence-309970-        9970 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway destination clone of TurboID-V5 (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-TurboID-V5-STOP_IDG-K
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(124..192,193..984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1155..1743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1644..1663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1897..1914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2031..2052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2067..2072,2073..2090,2091..2097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2105..2121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2110..2132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2129..2145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2129..2145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2164..2184
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2166..2184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2212..2438
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2439..2619
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2666..2791
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3284..3517
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3702..3746
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4013..4129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4163..4183
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4183..5394
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5231..5250
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5398..5418)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             5422..5690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5700..5741
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5739..5763
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5766..5775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5772..6734
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MGKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6741..6782
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(6787..6811)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6841..7429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6894..6914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7312..7323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7443..7942
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7478..7501)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7875..7894
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7963..8562
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7963..7982)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8459..8479
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8700..8933
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9005..9126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9042..9061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9096..9115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9166..9301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9321..9340)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9322..9340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9350..9367)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9350..9366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9359..9381)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9508..9963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9595..9614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9805..9826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
       61 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      121 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      181 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      241 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      301 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      361 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      421 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      481 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      541 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      601 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
      661 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
      721 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
      781 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
      841 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
      901 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
      961 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1861 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1921 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2041 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2101 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2161 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     2221 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     2281 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     2341 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     2401 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     2461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     2581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     2641 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     2701 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     2761 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     2821 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     2881 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     2941 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3001 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3061 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3121 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     3181 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     3241 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     3301 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     3361 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     3421 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     3481 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     3541 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     3601 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     3661 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     3721 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     3781 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     3841 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     3901 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     3961 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     4021 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4081 acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgtccagtg
     4141 tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg cgccgggttt
     4201 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     4261 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     4321 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     4381 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     4441 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     4501 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     4561 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     4621 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     4681 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     4741 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     4801 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     4861 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     4921 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     4981 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     5041 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     5101 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     5161 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     5221 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     5281 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     5341 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacggccaa
     5401 cttttgtata caaagtggcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5461 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5521 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5581 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5641 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc ggcaccatgg
     5701 gtaagcctat ccctaaccct ctcctcggtc tcgattctac aactttgtac aaaaaagcag
     5761 gcttagccac catgggaaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta
     5821 atggcgagtt ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta
     5881 tcaacaagca cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa
     5941 agggctactc tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc
     6001 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     6061 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     6121 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     6181 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     6241 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     6301 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     6361 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     6421 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     6481 ctctggccgc tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     6541 gcctggctcc atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga
     6601 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     6661 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     6721 ggtctgccga aaaggctagc ggcaagccca tccccaaccc cctgctgggc ctggacagca
     6781 cctaaaaccc agctttcttg tacaaagttg tgatatccag cacagtggcg gccgctcgac
     6841 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6901 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6961 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7021 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7081 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7141 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7201 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7261 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7321 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7381 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
     7441 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     7501 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     7561 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     7621 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     7681 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     7741 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     7801 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     7861 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     7921 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     7981 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8041 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8101 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8161 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8221 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8281 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8341 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8401 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8461 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8521 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     8581 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     8641 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8701 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     8761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8941 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9001 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9121 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     9181 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9241 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9301 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     9361 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     9421 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     9481 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     9541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9721 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9841 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9901 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     9961 tttaggtggc
//
