LOCUS       sequence-310006-       11642 bp    DNA     circular SYN 23-MAY-2025
DEFINITION  Lentivirus for doxycycline inducible expression of nontargeting 
            shRNA, GFP marker, based on Addgene 11652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11642)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Dox nontargeting shRNA GFP
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             12..645
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    692..817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1310..1543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1728..1772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2039..2156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2195..2694
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2230..2253)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2627..2646
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2745..2754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2751..2753,2754..2756,2757..3467)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2751..2753,2754..2756,2757..3467)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2796..2817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3057..3076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3404..3425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3545..4112
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3563..4114
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3707..3724)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3934..3953
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4738..4758
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4861..5055
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5194..5210)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5195..5211)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5231..5819
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5284..5304)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5702..5713)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5987..6005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6029..6047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6071..6089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(6215..6248)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6268..6448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6473..6491)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6474..6491)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6781..6800)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7014..7033)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7133..7155
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7193..7211)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7279..7383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7384..7452,7453..8244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7602..7621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8415..9003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8904..8923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9157..9174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9244..9264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9249..9578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9429..9564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10712..10777
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10907..10927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11352..11486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11389..11408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11443..11462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
       61 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
      121 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
      181 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
      241 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
      301 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
      361 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
      421 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
      481 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      541 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      601 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      661 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      721 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      781 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      841 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      901 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      961 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1021 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1081 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1141 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1621 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1681 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1741 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1801 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1861 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1921 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1981 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     2041 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2101 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     2161 atcacgagac tagcctcgac gatggtcgag taccgggtag gggaggcgct tttcccaagg
     2221 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     2281 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     2341 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     2401 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     2461 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     2521 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     2581 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     2641 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctct
     2701 agcgggatcc aggcctaagc ttacgcgtcc tagcgctacc ggtcgccacc atggtgagca
     2761 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2821 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2881 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2941 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3001 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3061 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3121 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3181 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3241 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3301 agcagaacac ccccatcggm gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3361 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3421 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     3481 ctcgactagc tagtagctag ctagctagtc gagctcaagc ttcgaattcc cgggctgcag
     3541 gaattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     3601 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     3661 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     3721 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     3781 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     3841 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     3901 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     3961 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     4021 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
     4081 acgtggtttt cctttgaaaa acacgatgat aataccatgg ctagactgga caagagcaaa
     4141 gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct gacgacaagg
     4201 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     4261 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     4321 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt
     4381 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     4441 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     4501 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     4561 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     4621 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     4681 ggcctggaac taatcatatg cggattagaa aaacaactta aatgtgaaag tgggtcgcca
     4741 aaaaagaaga gaaaggtcga cggcggtggt gctttgtctc ctcagcactc tgctgtcact
     4801 caaggaagta tcatcaagaa caaggagggc atggatgcta agtcactaac tgcctggtcc
     4861 cggacactgg tgaccttcaa ggatgtattt gtggacttca ccagggagga gtggaagctg
     4921 ctggacactg ctcagcagat cgtgtacaga aatgtgatgc tggagaacta taagaacctg
     4981 gtttccttgg gttatcagct tactaagcca gatgtgatcc tccggttgga gaagggagaa
     5041 gagccctggc tggtggagag agaaattcac caagagaccc atcctgattc agagactgca
     5101 tttgaaatca aatcatcagt ttaaggatct cgactagcta gtagctagct agctagtcga
     5161 gctcaagctt cgaattcgat atcaagctta tcgcgatacc gtcgacctcg acctcgacta
     5221 gtcatatgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5281 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5341 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5401 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5461 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5521 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5581 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     5641 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5701 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5761 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5821 tcggtacgta tggccaggta cctttaagac caatgactta caaggcagct gtagatctta
     5881 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5941 atgggatcaa ttcaccatgc tagtggatcc ccctcgagtt taccactccc tatcagtgat
     6001 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     6061 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     6121 agygatagag aaaagtgmaa gtcgagttta ccactcccta tcagcactag agaaaagtga
     6181 aagtcgagty tacmmcyycc tatcagtgat agagaaaact tcgtatagca tacattatac
     6241 gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6301 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6361 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6421 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     6481 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     6541 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     6601 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     6661 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     6721 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     6781 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     6841 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     6901 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     6961 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     7021 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     7081 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7141 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     7201 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8281 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8521 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     9301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9481 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9541 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     9601 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     9661 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     9721 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     9781 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     9841 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     9901 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     9961 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
    10021 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
    10081 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
    10141 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
    10201 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
    10261 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
    10321 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
    10381 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
    10441 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
    10501 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
    10561 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
    10621 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
    10681 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
    10741 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
    10801 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
    10861 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
    10921 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
    10981 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
    11041 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
    11101 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
    11161 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
    11221 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
    11281 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    11341 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11461 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11521 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11581 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11641 ca
//
