LOCUS       sequence-310093-        6633 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-S
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(149..176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(268..354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     403..425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     417..434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     418..434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    450..549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             549..569
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(4393..4492)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4509..4528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4510..4528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4533..4549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4533..4549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4980..5771
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5864..6452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6353..6372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6606..6623
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
      421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      541 aagttggcga aaacctgtac ttccaaagta tgttcgtgtt cctggtgctg ctgccactgg
      601 tgagctccca gtgcgtgaac ctgaccacca gaacccagct gccacctgcc tacaccaact
      661 ccttcaccag gggcgtgtac tacccagaca aggtgttcag atccagcgtg ctgcactcta
      721 cccaggacct gttcctgcca ttcttctcta acgtgacctg gttccacgcc atccacgtgt
      781 ctggaaccaa cggcaccaag agattcgaca acccagtgct gccattcaac gacggcgtgt
      841 acttcgcttc taccgagaag tctaacatca tcaggggttg gatcttcgga accaccctgg
      901 actctaagac ccagtctctg ctgatcgtga acaacgctac caacgtggtg atcaaggtgt
      961 gcgagttcca gttctgcaac gacccattcc tgggagtgta ctaccacaag aacaacaagt
     1021 cttggatgga aagcgagttc agggtgtaca gctctgctaa caactgcacc ttcgagtacg
     1081 tgtctcagcc tttcctgatg gacctggaag gaaagcaggg aaacttcaag aacctgagag
     1141 agttcgtgtt caagaacatc gacggttact tcaagatata cagcaagcac accccaatca
     1201 acctggtgag ggacctgcca cagggtttct ctgctctgga gcccctggtg gacctgccaa
     1261 tcggcatcaa catcaccagg ttccagaccc tgctggctct gcacaggtct tacctgaccc
     1321 caggcgacag cagctctgga tggaccgccg gcgccgctgc ttactacgtg ggatacctgc
     1381 agccaaggac cttcctgctg aagtacaacg aaaacggcac catcaccgac gccgtggact
     1441 gcgctctgga cccactgtct gagaccaagt gcaccctgaa gtctttcacc gtggaaaagg
     1501 gaatctacca gaccagcaac ttccgcgtgc agcccaccga gtctatcgtg agattcccaa
     1561 acatcaccaa cctgtgccca ttcggagaag tgttcaacgc taccaggttc gccagcgtgt
     1621 acgcttggaa caggaagaga atcagcaact gcgtggccga ctactctgtg ctgtacaact
     1681 ctgcctcctt ctccaccttc aagtgctacg gcgtgtcccc aaccaagctg aacgacctgt
     1741 gcttcaccaa cgtgtacgct gactccttcg tgatcagagg agacgaagtg agacagatcg
     1801 ctcccggtca gaccggaaag atcgccgact acaactacaa gctgccagac gacttcaccg
     1861 gttgcgtgat cgcttggaac tctaacaacc tggactctaa ggtgggaggc aactacaact
     1921 acctgtacag gctgttccgc aagagcaacc tgaagccttt cgaaagggac atcagcaccg
     1981 aaatctacca ggctggatct accccatgca acggagtgga aggattcaac tgctacttcc
     2041 ctctgcagtc ttacggattc cagccaacca acggagtggg ataccagcca tacagagtgg
     2101 tggtgctgtc tttcgagctg ctgcacgctc ctgctaccgt gtgcggacca aagaagtcta
     2161 ccaacctggt gaagaacaag tgcgtgaact tcaacttcaa cggactgacc ggaaccggag
     2221 tgctgaccga atccaacaag aagttcctgc ctttccagca gttcggtaga gacatcgccg
     2281 acaccaccga cgctgtgaga gacccacaga ccctggaaat cctggacatc accccctgct
     2341 ctttcggagg agtgagcgtg atcaccccag gaaccaacac ctccaaccag gtggccgtgc
     2401 tgtaccagga cgtgaactgc accgaagtgc cagtggccat ccacgctgac cagctgaccc
     2461 caacctggag agtgtacagc accggatcta acgtgttcca gacccgtgcc ggctgcctga
     2521 tcggagccga gcacgtgaac aactcctacg aatgcgacat cccaatcgga gctggcatct
     2581 gcgcttctta ccagacccag accaacagcc caagaagggc cagatccgtg gcctctcaga
     2641 gcatcatcgc ctacaccatg agcctgggcg ctgaaaacag cgtggcttac agcaacaact
     2701 ctatcgccat cccaaccaac ttcaccatct ctgtgaccac cgaaatcctg ccagtgagca
     2761 tgaccaagac cagcgtggac tgcaccatgt acatctgcgg agactctacc gaatgctcta
     2821 acctgctgct gcagtacggc agcttctgca cccagctgaa ccgcgctctg accggaatcg
     2881 ctgtggagca ggacaagaac acccaggaag tgttcgctca ggtgaagcag atatacaaga
     2941 ccccacctat caaggacttc ggaggattca acttctctca gatcctgccc gacccatcta
     3001 agccctctaa gaggtctttc atcgaagacc tgctgttcaa caaggtgacc ctggccgacg
     3061 ccggtttcat caagcagtac ggtgactgcc tgggagacat cgccgctaga gacctgatct
     3121 gcgcccagaa gttcaacggt ctgaccgtgc tgccaccact gctgaccgac gagatgatcg
     3181 cccagtacac ctctgccctg ctggctggaa ccatcacctc tggttggacc ttcggagctg
     3241 gcgccgccct gcagatccca ttcgctatgc agatggccta ccgtttcaac ggtatcggag
     3301 tgacccagaa cgtgctgtac gaaaaccaga agctgatcgc taaccagttc aactctgcta
     3361 tcggaaagat ccaggactcc ctgtcctcta ccgcttctgc cctgggaaag ctgcaggacg
     3421 tggtgaacca gaacgcccag gccctgaaca ccctggtgaa gcagctgtct tctaacttcg
     3481 gagccatctc ctctgtgctg aacgacatcc tgtctaggct ggacaaggtg gaagccgagg
     3541 tgcagatcga cagactgatc accggaagac tgcagtctct gcagacctac gtgacccagc
     3601 agctgatcag ggccgctgaa atcagagctt ctgccaacct ggctgccacc aagatgtccg
     3661 aatgcgtgct gggccagtct aagagggtgg acttctgcgg aaagggatac cacctgatgt
     3721 ccttcccaca gtctgctcca cacggagtgg tgttcctgca cgtgacctac gtgcccgccc
     3781 aggaaaagaa cttcaccacc gccccagcta tctgccacga cggaaaggct cacttcccca
     3841 gggagggagt gttcgtgtct aacggaaccc actggttcgt gacccagagg aacttctacg
     3901 agccacagat catcaccacc gacaacacct tcgtgtctgg aaactgcgac gtggtgatcg
     3961 gaatcgtgaa caacaccgtg tacgacccac tgcagccaga actggactct ttcaaggagg
     4021 aactggacaa gtacttcaag aaccacacct ctccagacgt ggacctggga gacatctccg
     4081 gaatcaacgc ctctgtggtg aacatccaga aggaaatcga cagactgaac gaggtggcca
     4141 agaacctgaa cgaatctctg atcgacctgc aggaactggg aaagtacgag cagtacatca
     4201 agtggccctg gtacatctgg ctgggtttca tcgctggtct gatcgctatc gtgatggtga
     4261 ccatcatgct gtgctgcatg acctcttgct gctcttgcct gaagggatgc tgctcttgcg
     4321 gatcttgctg caagttcgac gaggacgact ccgagcctgt gctgaagggt gtgaagctgc
     4381 actacaccta atacccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
     4441 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     4501 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     4561 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
     4621 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     4681 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     4741 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     4801 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     4861 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     4921 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     4981 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     5041 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     5101 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     5161 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     5221 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     5281 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     5341 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     5401 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     5461 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     5521 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     5581 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     5641 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     5701 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     5761 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     5821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6601 agcgcagcga gtcagtgagc gaggaagcgg aag
//
