LOCUS       sequence-310390-       14970 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway cloning compatible ratiometric binary vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14970)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO2251
FEATURES             Location/Qualifiers
     source          1..14970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1614..1634
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1650..1774
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1811..1816,1817..1834,1835..1841)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1895..2554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1961..1980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2896..3201
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3080..3099
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3242..3366)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5120..5185
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     terminator      5907..6159
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6192..6208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6192..6208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6205..6227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6216..6232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6240..6246,6247..6264,6265..6270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6285..6306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(6340..6592)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6982..7776)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7093..7112)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7703..7722
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7797..7980)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(7833..8012)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    8186..8210
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8731..9522
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9768..10356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10257..10276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10510..10527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10542..10682
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10608..10627)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10768..10790
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11026..11220
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11286..12359)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12788..13417)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    14718..14742
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14930..14952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14944..14961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14945..14961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgtcgta tgatcgcgaa gcacccaccc taagccacat tttaatcctt ctgttggcca
       61 tgccccatca aagttgcact taacccaaga ttgtggtgga gcttcccatg tttctcgtct
      121 gtcccgacgg tgttgtggtt ggtgctttcc ttacattctg agcctctttc cttctaatcc
      181 actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat tccctccctt
      241 taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg actaaagcct
      301 ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg aggtttgtat
      361 gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat tcaaagaatg
      421 ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg tcattggact
      481 gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat atgaattaaa
      541 taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact tgttcaccaa
      601 cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata tccaataaca
      661 ctaaaaaatt aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt
      721 tccaagaaat tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca
      781 aaaaggaaaa gaaataaagc acgaagaatt ccagaaaata cgaaatacgc ttcaatgcag
      841 tgggacccac ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa
      901 cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat
      961 gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg
     1021 cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta
     1081 aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca
     1141 ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct
     1201 tttcttcttc ttcttctata aaacaatacc caaagagttc ttcttcttca caattcagat
     1261 ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa
     1321 tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg
     1381 tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga
     1441 tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa
     1501 ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatcc agatctggtg
     1561 ttagtttcta gtttgtgcga tcgaatttgt cctcgagagg ggactctaga atgccaaaaa
     1621 agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct gaacgagaaa
     1681 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1741 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1801 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1861 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1921 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1981 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2041 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2101 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2161 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2221 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2281 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2341 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2401 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2461 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2521 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2581 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2641 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt
     2701 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2761 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2821 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2881 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2941 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3001 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3061 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3121 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3181 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     3241 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     3301 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     3361 agtggtgggc ccaatggaag atgctaagaa tattaagaag ggaccagctc ctttctaccc
     3421 actcgaagat ggaacagcag gtgaacagtt acacaaggct atgaaaagat atgctcttgt
     3481 tcctggtact atcgcattca cagatgctca tatcgaggtg gatataactt atgctgagta
     3541 cttcgaaatg tctgttaggc tcgctgaagc tatgaagaga tacggattaa atacaaacca
     3601 caggattgtt gtgtgtagtg aaaattcttt gcaatttttc atgcctgttc ttggagcttt
     3661 gttcatcggt gtggctgttg caccagctaa tgatatatat aacgagagag aacttcttaa
     3721 ttctatggga atttctcaac ctactgttgt gttcgtgtct aagaaaggac ttcaaaagat
     3781 tttgaacgtt cagaagaaac ttccaattat ccagaagatt attattatgg attcaaagac
     3841 agattaccaa ggtttccagt caatgtacac ctttgtgacc agtcatttgc ctcctggatt
     3901 caatgagtac gatttcgttc cagaaagttt tgatagagat aagactattg ctctcattat
     3961 gaactcttca ggatctacag gtcttcctaa gggtgtggca ttgccacata gaaccgcttg
     4021 tgttaggttc tctcacgcaa gagatcctat tttcggaaac cagataattc cagatacagc
     4081 tatcttgtca gttgtgcctt tccatcacgg atttggaatg ttcactacac tcggatacct
     4141 tatttgtggt tttagggttg tgctcatgta cagattcgaa gaggaactct ttttaagatc
     4201 tttacaagat tataagatac agtcagcact cttagttcca acccttttct ctttctttgc
     4261 taagtcaact ttgatcgata agtacgatct ttctaacttg catgagattg caagtggagg
     4321 tgctcctctt tctaaagaag tgggtgaagc agttgctaag aggtttcact tgccaggaat
     4381 cagacaagga tatggtctta cagagaccac ttctgctatc ttgataaccc ctgaaggaga
     4441 tgataaacca ggagcagttg gaaaggttgt tcctttcttt gaagctaaag ttgtggatct
     4501 cgatacagga aagaccttag gtgtgaatca gaggggagag ctttgcgtta gaggtcctat
     4561 gataatgagt ggatatgtta ataacccaga agcaactaac gctcttatcg ataaagatgg
     4621 atggttgcat tcaggagata ttgcttactg ggatgaggat gaacactttt tcatagtgga
     4681 taggctcaag tcattaatta agtataaggg ataccaagtt gcacctgctg aactcgaaag
     4741 tattcttttg cagcatccaa acatcttcga tgcaggagtt gctggtcttc ctgatgatga
     4801 tgctggagaa ctcccagctg cagttgtggt tttagaacac ggaaagacta tgacagagaa
     4861 agaaattgtg gattacgttg catctcaagt tacaaccgct aagaaactta gaggtggtgt
     4921 ggtttttgtg gatgaggttc caaagggact cactggtaaa ttagatgcta gaaagatcag
     4981 ggaaattttg atcaaagcaa agaaaggagg aaagattgct gttacgcgtt ctaatgccgc
     5041 cgatgaggtt gcgactggat caggtctttt agcgatacat ccgaccgaag cgcgacataa
     5101 acaaaaaatt gtagcgccgg tcaaacaaac cctgaatttc gacttactca aacttgcggg
     5161 tgacgtagag tcaaatccag ggcctggtac catggtgtcc aagggcgaag ctgtgattaa
     5221 agaatttatg cgttttaagg tccacatgga aggatccatg aatggacatg aattcgaaat
     5281 cgagggggag ggcgagggcc gaccttatga gggtacacag accgcgaagc taaaggtaac
     5341 caagggaggc ccactaccct ttagttggga catactcagc ccgcagttta tgtatggcag
     5401 tcgtgccttt ataaagcacc cagcggatat accagactac tacaaacagt ccttcccaga
     5461 ggggtttaaa tgggagcgag tgatgaactt tgaggacggt ggtgctgtga cggtaactca
     5521 ggacacatca ctcgaagacg ggactcttat ttacaaagta aaactgagag ggaccaattt
     5581 tccaccagat ggccccgtca tgcaaaaaaa gacgatgggt tgggaagcat ctacagaacg
     5641 actctatccc gaggacggcg tcctcaaagg cgacattaaa atggccttga ggttgaaaga
     5701 cggtggacgt tacttggcgg acttcaaaac aacttataaa gcaaaaaagc cagtgcaaat
     5761 gcctggagcg tacaatgtag atagaaaact tgatataacg tcacataatg aggactatac
     5821 cgtggttgag caatacgaga ggtcagaagg acgacacagc accggcggta tggacgagtt
     5881 gtacaagtaa gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     5941 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     6001 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     6061 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     6121 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa
     6181 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6241 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6301 acattaggct gaattaggcg cgcctatttc tgaattcccg atctagtaac atagatgaca
     6361 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     6421 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     6481 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     6541 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggatca
     6601 tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata tcgcggtgca
     6661 tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg ggcccgatca
     6721 tattgtcgct caggatcgtg gcgttgtgct tgtcggccgt tgctgtcgta atgatatcgg
     6781 caccttcgac cgcctgttcc gcagagatcc cgtgggcgaa gaactccagc atgagatccc
     6841 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     6901 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     6961 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     7021 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     7081 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     7141 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     7201 gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg ccttgagcct
     7261 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     7321 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     7381 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     7441 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     7501 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     7561 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     7621 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     7681 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     7741 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatccag atccggtgca
     7801 gattatttgg attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
     7861 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
     7921 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
     7981 gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
     8041 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatgaattc
     8101 agaaataaat tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
     8161 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     8221 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     8281 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     8341 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     8401 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga
     8461 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     8521 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     8581 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     8641 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     8701 ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg
     8761 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     8821 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     8881 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     8941 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     9001 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     9061 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     9121 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     9181 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     9241 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     9301 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     9361 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     9421 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     9481 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg
     9541 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat
     9601 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     9661 taagccctac acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt
     9721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9901 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10381 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10441 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10501 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
    10561 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
    10621 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
    10681 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
    10741 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    10801 catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac
    10861 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
    10921 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
    10981 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
    11041 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
    11101 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
    11161 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
    11221 ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
    11281 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
    11341 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
    11401 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
    11461 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
    11521 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
    11581 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
    11641 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
    11701 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
    11761 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
    11821 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
    11881 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
    11941 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
    12001 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
    12061 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
    12121 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc
    12181 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
    12241 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
    12301 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg
    12361 ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc
    12421 ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg
    12481 gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt
    12541 catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc
    12601 ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag
    12661 ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg
    12721 cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct
    12781 cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag
    12841 cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc
    12901 tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg
    12961 caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta
    13021 gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc
    13081 atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg
    13141 acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga
    13201 tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat
    13261 cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa
    13321 catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg
    13381 acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt
    13441 atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac
    13501 acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc
    13561 cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg
    13621 cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat
    13681 gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca
    13741 ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc
    13801 cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag
    13861 cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc
    13921 tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct
    13981 cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac
    14041 tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc
    14101 cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc
    14161 agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca
    14221 gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc
    14281 ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag
    14341 tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc
    14401 atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg
    14461 acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc
    14521 gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag
    14581 cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
    14641 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
    14701 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
    14761 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc gccattcagg
    14821 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    14881 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    14941 cgttgtaaaa cgacggccag tgccaagctt
//
