LOCUS       sequence-310426-        9242 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses human MUC2 residues 21-1259.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Javitt G, Khmelnitsky L, Albert L, Bigman LS, Elad N, Morgenstern D,
            Ilani T, Levy Y, Diskin R, Fass D
  TITLE     Assembly Mechanism of Mucin and von Willebrand Factor Polymers.
  JOURNAL   Cell. 2020 Oct 29;183(3):717-729.e16. doi: 
            10.1016/j.cell.2020.09.021. Epub 2020 Oct 7.
  PUBMED    33031746
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.09.021"; journalName: "Cell"; date: 
            "2020-10-29- 29"; volume: "183"; issue: "3"; pages: "717-729.e16"
COMMENT     Sequence Label: pCDNA3.1-Muc2D1D2D3
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4508..4525
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4599..4616)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4605..4829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4875..5303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4962..4981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5172..5193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5312..5332)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5317..5646
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5497..5632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5559..5578
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5713..6507
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5767..5786)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6377..6396
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6681..6802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6718..6737)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6772..6791
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6851..6867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6851..6867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6864..6886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6875..6891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6899..6905,6906..6923,6924..6929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6944..6965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7082..7099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7253..7841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7333..7352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8012..8803,8804..8872))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8635..8654
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8873..8977)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9052..9071)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc atgaggaggt gcaacagcgg
      721 ctccgggccg ccgccgtcgc tgctgctgct gctgtggctg ctcgcggttc ccggcgctaa
      781 cgcggccccg tcggagctcc agacagaggg cagaacccga aaccacggcc acaacgtctg
      841 cagcacctgg ggcaacttcc actacaagac cttcgacggg gacgtcttcc gcttccccgg
      901 cctctgcgac tacaacttcg cctccgactg ccgaggctcc tacaaggaat ttgctgtgca
      961 cctgaagcgg ggtccgggcc aggctgaggc ccccgccggg gtggagtcca tcctgctgac
     1021 catcaaggat gacaccatct acctcacccg ccacctggct gtgcttaacg gggccgtggt
     1081 cagcaccccg cactacagcc ccgggctgct cattgagaag agcgatgcct acaccaaagt
     1141 ctactcccgc gccggcctca ccctcatgtg gaaccgggag gatgcactca tgctggagct
     1201 ggacactaag ttccggaacc acacctgtgg cctctgcggg gactacaacg gcctgcagag
     1261 ctattcagaa ttcctctctg acggcgtgct cttcagtccc ctggagtttg ggaacatgca
     1321 gaagatcaac cagcccgatg tggtgtgtga ggatcccgag gaggaggtgg cccccgcatc
     1381 ctgctccgag caccgcgccg agtgtgagag gctgctgacc gccgaggcct tcgcggactg
     1441 tcaggacctg gtgccgctgg agccgtatct gcgcgcctgc cagcaggacc gctgccggtg
     1501 cccgggcggt gacacctgcg tctgcagcac cgtggccgag ttctcccgcc agtgctccca
     1561 cgccggcggc cggcccggga actggaggac cgccacgctc tgccccaaga cctgccccgg
     1621 gaacctggtg tacctggaga gcggctcgcc ctgcatggac acctgctcac acctggaggt
     1681 gagcagcctg tgcgaggagc accgcatgga cggctgtttc tgcccagaag gcaccgtata
     1741 tgacgacatc ggggacagtg gctgcgttcc tgtgagccag tgccactgca ggctgcacgg
     1801 acacctgtac acaccgggcc aggagatcac caatgactgc gagcagtgtg tctgtaacgc
     1861 tggccgctgg gtgtgcaaag acctgccctg ccccggcacc tgtgccctgg aaggcggctc
     1921 ccacatcacc accttcgatg ggaagacgta caccttccac ggggactgct actatgtcct
     1981 ggccaagggt gaccacaacg attcctatgc tctcctgggc gagctggccc cctgtggctc
     2041 cacagacaag cagacctgcc tgaagacggt ggtgctgctg gctgacaaga agaagaatgt
     2101 ggtggtcttc aagtccgatg gcagtgtact gctcaacgag ctgcaggtga acctgcccca
     2161 cgtgaccgcg agcttctctg tcttccgccc gtcttcctac cacatcatgg tgagcatggc
     2221 cattggcgtc cggctgcagg tgcagctggc cccagtcatg caactctttg tgacactgga
     2281 ccaggcctcc caggggcagg tgcagggcct ctgcgggaac ttcaacggcc tggaaggtga
     2341 cgacttcaag acggccagcg ggctggtgga ggccacgggg gccggctttg ccaacacctg
     2401 gaaggcacag tcaagctgcc atgacaagct ggactggttg gacgatccct gctccctgaa
     2461 catcgagagc gccaactacg ccgagcactg gtgctccctc ctgaagaaga cagagacccc
     2521 ctttggcagg tgccactcgg ctgtggaccc tgctgagtat tacaagaggt gcaaatatga
     2581 cacgtgtaac tgtcagaaca atgaggactg cctgtgcgcc gccctgtcct cctacgcgcg
     2641 cgcctgcacc gccaagggcg tcatgctgtg gggctggcgg gagcatgtct gcaacaagga
     2701 tgtgggctcc tgccccaact cgcaggtctt cctgtacaac ctgaccacct gccagcagac
     2761 ctgccgctcc ctctccgagg ccgacagcca ctgtctcgag ggctttgcgc ctgtggacgg
     2821 ctgcggctgc cctgaccaca ccttcctgga cgagaagggc cgctgcgtac ccctggccaa
     2881 gtgctcctgt taccaccgcg gtctctacct ggaggcgggg gatgtggtcg tcaggcagga
     2941 agaacgatgt gtgtgccggg atgggcggct gcactgtagg cagatccggc tgatcggcca
     3001 gagctgcacg gccccaaaga tccacatgga ctgcagcaac ctgactgcac tggccacctc
     3061 gaagccccga gccctcagct gccagacgct ggccgccggc tattaccaca cagagtgtgt
     3121 cagtggctgt gtgtgccccg acgggctgat ggatgacggc cggggtggct gtgtggtgga
     3181 gaaggaatgc ccttgcgtcc ataacaacga cctgtattct tccggcgcca agatcaaggt
     3241 ggactgcaat acctgcacct gcaagagagg acgctgggtg tgcacccagg ctgtgtgcca
     3301 tggcacctgc tccatttacg ggagtggcca ctacatcacc tttgatggga agtactacga
     3361 ctttgacgga cactgctcct acgtggctgt tcaggactac tgcggccaga actcctcact
     3421 gggctcattc agcatcatca ccgagaacgt cccctgtggc actacgggcg tcacctgctc
     3481 caaggccatt aagatcttca tggggaggac ggagctgaag ttggaagaca agcaccgtgt
     3541 ggtgatccag cgtgatgagg gtcaccacgt ggcctacacc acgcgggagg tgggccagta
     3601 cctggtggtg gagtccagca cgggcatcat cgtcatctgg gacaagagga ccaccgtgtt
     3661 catcaagctg gctccctcct acaagggcac cgtgtgtggc ctgtgtggga actttgacca
     3721 ccgctccaac aacgacttca ccacgcggga ccacatggtg gtgagcagcg agctggactt
     3781 cgggaacagc tggaaggagg cccccacctg cccagatgtg agcaccaacc ccgagccctg
     3841 cagcctgaac ccgcaccgcc gctcctgggc cgagaagcag tgcagcatcc tcaaaagcag
     3901 cgtgttcagc atctgccaca gcaaggtgga ccccaagccc ttctacgagg cctgtgtgca
     3961 cgactcgtgc tcctgtgaca cgggtgggga ctgtgagtgc ttctgctctg ccgtggcctc
     4021 ctacgcccag gagtgtacca aagagggggc ctgcgtgttc tggaggacgc cggacctgtg
     4081 ccccatattc tgcgactact acaaccctcc gcatgagtgt gagtggcact atgagccatg
     4141 tgggaaccgg agcttcgaga cctgcaggac catcaacggc atccactcca acatctccgt
     4201 gtcctacctg gagggctgct acccccggtg ccccaaggac aggcccatct atgaggagga
     4261 tctgaagaag tgtgtcactg cagacaagtg tggctgctat gtcgaggaca cccactaccc
     4321 acctggagca tcggttccca ccgaggagac ctgcaagtcc tgcgtgtgta ccaactcctc
     4381 ccaagtcgtc tgcaggccgg aagaaggaaa gattcttaac cagacccagg atggcgcctt
     4441 ctgctactgg gagatctgtg gccccaacgg gacggtggag aagcacttca acatctgttc
     4501 cattacgcac catcatcacc atcattgaga attctgcaga tatccagcac agtggcggcc
     4561 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4621 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4681 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4741 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4801 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4861 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4921 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4981 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5041 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5101 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5161 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5221 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5281 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     5341 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5401 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5461 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5521 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5581 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5641 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     5701 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5761 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5821 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5881 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5941 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6001 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6061 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6121 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6181 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6241 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6301 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6361 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6421 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6481 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     6541 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6601 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6661 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6781 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7021 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7681 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7921 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7981 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8041 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8101 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8161 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8221 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8281 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8341 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8401 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8461 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8521 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8581 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8641 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8701 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8761 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8821 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8881 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8941 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9001 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     9061 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     9121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     9181 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     9241 tt
//
