LOCUS       sequence-31050-e         873 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: pDEST51-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg
      121 tcgaattcgc cctttttctc aacagatgtt cgtctcattt tttcagctaa ttcttttctc
      181 acttcaatgt gtttttctaa attttgcact tcatgtggaa gattgatgtc ccaaacggtc
      241 aagcaagatt gtatctcttt ctgcttttgt gtaccttcag tatttttccg gttgtagccc
      301 aataccagca tgacattttt aaggcttcct agctgtgctg tcatcatgac tctgaccgaa
      361 ttacaaaagt tgtaaattac acgtataagt cgacgagttg aaagatccag gagtaaaata
      421 tggcctcctc cagttcctat ccaaagagca gtgttcttct gaaggcagag ggttttcact
      481 ctcccagaat aagacatttt gtgttttgat tccttgtttt cttttaccat tacctccctt
      541 aaaaagtgca cgcagtctat tagtccacag agtttttcag ttttcttatc ccacacttcc
      601 acaacagggc tattttgctt agcaatatag agagcagtgt ctaccaccac tgttatgatg
      661 ttggaatcac tgaaagctgc ataagaaaac agttggcttg ttcttgtctc aatgagtttc
      721 tgaatggtga aatcattaga aaaggagaaa atctttgtgc cacatcctcc ccacattaca
      781 tttctttccg ttgaatttgt ggattcactc aaacacatca atggagtact gacatttcct
      841 atatttagta tcttcaaagg agcagctcct tta
//
