LOCUS       sequence-310893-        6201 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Produces a fusion between Aquamarine and superYFP. It can be used as
            a positive control for FRET (using FLIM).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard
            O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier 
            M
  TITLE     Aurora kinase A localises to mitochondria to control organelle 
            dynamics and energy production.
  JOURNAL   eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111.
  PUBMED    30070631
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-08-2"; volume: "7. pii: 38111. doi"; pages: " 
            10.7554/eLife.38111"
COMMENT     Sequence Label: pCMV Aquamarine-superYFP tandem
FEATURES             Location/Qualifiers
     source          1..6201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(1309..1317,1318..1332)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1378..2037
                     /codon_start=1
                     /product="tetrameric yellow fluorescent protein from 
                     Branchiostoma lanceolatum, also known as blFP-Y3"
                     /label=LanYFP
                     /translation="MASLPATHELHIFGSFNGVDFDMVGRGTGNPNDGYEELNLKSTKG
                     DLQFSPWILVPQIGYGFHQYLPFPDGMSPFQAAMKDGSGYQVHRTMQFEDGASLTSNYR
                     YTYEGSHIKGEFQVKGTGFPADGPVMTNSLTAADWCVTKMLYPNDKTIISTFDWTYTTG
                     NGKRYQSTARTTYTFAKPMAANILKNQPMFVFRKTELKHSKTELNFKEWQKAFTDVM"
     CDS             2089..2748
                     /codon_start=1
                     /product="tetrameric yellow fluorescent protein from 
                     Branchiostoma lanceolatum, also known as blFP-Y3"
                     /label=LanYFP
                     /translation="MASLPATHELHIFGSFNGVDFDMVGRGTGNPNDGYEELNLKSTKG
                     DLQFSPWILVPQIGYGFHQYLPFPDGMSPFQAAMKDGSGYQVHRTMQFEDGASLTSNYR
                     YTYEGSHIKGEFQVKGTGFPADGPVMTNSLTAADWCVTKMLYPNDKTIISTFDWTYTTG
                     NGKRYQSTARTTYTFAKPMAANILKNQPMFVFRKTELKHSKTELNFKEWQKAFTDVM"
     polyA_signal    2929..3050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2966..2985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3020..3039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3057..3512)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3194..3215)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3406..3425
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3539..3643
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3645..4002
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3645..3836
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3668..3688)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3853..3988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4037..4831
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4037..4831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4091..4110)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4701..4720
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5019..5038)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5063..5110
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5439..6027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5928..5947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgagc tggggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac atcagcggca acgtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctgctagc gtcgacacca tgggtcggga tctgtacgac
     1321 gatgacgata aggatccccc cgctgaattc atggtctcca aaggagagga ggataacatg
     1381 gcctctctcc cagcgacaca tgagttacac atctttggct ccttcaacgg tgtggacttt
     1441 gacatggtgg gtcgtggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag
     1501 tccaccaagg gtgacctcca gttctccccc tggattctgg tccctcaaat cgggtatgga
     1561 ttccatcagt acctgccctt ccccgacggg atgtcgcctt tccaggccgc catgaaagat
     1621 ggctccggat accaagtcca tcgcacaatg cagtttgaag acggtgcctc cctgacttcc
     1681 aactaccgct acacctacga gggaagccac atcaaaggag agtttcaggt gaaggggact
     1741 ggtttccctg ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcgtg
     1801 accaagatgc tgtaccccaa cgacaaaacc atcatcagca cctttgactg gacttacacc
     1861 actggaaatg gcaagcgcta ccagagcact gcgcggacca cctacacctt tgccaagcca
     1921 atggcggcca acatcctgaa gaaccagccg atgttcgtgt tccgtaagac ggaactcaag
     1981 cactccaaga ccgaactcaa cttcaaggag tggcaaaagg catttaccga tgtgatgtcg
     2041 actggcactg gttctacggg ctcgggctcc tcaggagagg aggataacat ggcctctctc
     2101 ccagcgacac atgagttaca catctttggc tccttcaacg gtgtggactt tgacatggtg
     2161 ggtcgtggca ccggcaatcc aaatgatggt tatgaggagt taaacctgaa gtccaccaag
     2221 ggtgacctcc agttctcccc ctggattctg gtccctcaaa tcgggtatgg cttccatcag
     2281 tacctgccct tccccgacgg gatgtcgcct ttccaggccg ccatgaaaga tggctccgga
     2341 taccaagtcc atcgcacaat gcagtttgaa gacggtgcct ccctgacttc caactaccgc
     2401 tacacctacg agggaagcca catcaaagga gagtttcagg tgaaggggac tggtttccct
     2461 gctgacggtc ctgtgatgac caactcgctg accgctgcgg actggtgcgt gaccaagatg
     2521 ctgtacccca acgacaaaac catcatcagc acctttgact ggacttacac cactggaaat
     2581 ggcaagcgct accagagcac tgcgcggacc acctacacct ttgccaagcc aatggcggcc
     2641 aacatcctga agaaccagcc gatgttcgtg ttccgtaaga cggaactcaa gcactccaag
     2701 accgaactca acttcaagga gtggcaaaag gcatttaccg atgtgatggg catggacgag
     2761 ctgtacaagt aaagcggccg cgacggtacc gcgggcccgg gatccaccgg atctagataa
     2821 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     3061 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3121 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3181 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3241 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3301 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3361 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3421 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3481 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3661 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3721 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3841 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3901 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4021 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4081 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4141 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4201 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4261 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4321 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4381 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4441 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4501 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4561 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4621 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4681 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4741 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4801 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4861 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4921 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4981 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5041 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5101 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5161 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5221 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5281 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6121 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     6181 tagcccatat atggagttcc g
//
