LOCUS       sequence-311023-        9462 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fluorescent reporter for ATF4 translation, Negative Control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   van Galen P, Kreso A, Mbong N, Kent DG, Fitzmaurice T, Chambers JE, 
            Xie S, Laurenti E, Hermans K, Eppert K, Marciniak SJ, Goodall JC, 
            Green AR, Wouters BG, Wienholds E, Dick JE
  TITLE     The unfolded protein response governs integrity of the 
            haematopoietic stem-cell pool during stress.
  JOURNAL   Nature. 2014 Jun 12;510(7504):268-72. doi: 10.1038/nature13228. Epub
            2014 Apr 28.
  PUBMED    24776803
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13228"; journalName: "Nature"; date: "2014-06-12- 
            12"; volume: "510"; issue: "7504"; pages: "268-72"
COMMENT     Sequence Label: pSMALB-ATF4.12
FEATURES             Location/Qualifiers
     source          1..9462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..39)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    complement(473..506)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    521..545
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      982..991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(988..990,991..993,994..1707)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(988..990,991..993,994..1704)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1033..1054)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1294..1313)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(1724..1748)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1774..1790)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1774..1790)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1791..1824)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1830..2418
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1883..1903)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2301..2312)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2505..2738
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2810..2931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2971..3106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3126..3145)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3127..3145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3155..3172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3155..3171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3164..3186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3313..3768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3400..3419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3610..3631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3794..3898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3899..3967,3968..4759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4117..4136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4930..5518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5419..5438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5672..5689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5806..5827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5842..5847,5848..5865,5866..5872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5880..5896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5885..5907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5904..5920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5904..5920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5939..5959
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5941..5959
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        6060..6439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6440..6643
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6658..6838
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6885..7010
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7503..7736
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7921..7965
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8232..8349
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    8481..8615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8658..9359)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 agctctgctt atataggcct cccaccgtac acgcctacca ctagtaatga aagaccccac
       61 ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa
      121 accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca
      181 aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac
      241 cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct
      301 cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc
      361 tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct
      421 tcccgagctc tataaaagag ctcacaaccc ctcactcggc ttaattaaga tcataacttc
      481 gtatagcata cattatacga agttatccca gcttgatatc acaagtttgt acaaaaaagc
      541 aggctccgcg gccgccccct tcaccagctt ttctgcttgc tgtctgccgg tttgagttgt
      601 gcgctcgggt gtccctttcc tcttcccctc ccgcagggct tgcggccacc atagcgtatt
      661 agaggcagca gtgcctgcgg cagcgttggc ctttgcagcg gcggcagcag caccaggctc
      721 tgcagcggca acccccaccg gcctaagcca tggcgctctt cacgaaatcc agcagcagtg
      781 ttgctgtaac ggacaaagat accttcgagt taagcacatt cctggaatcc agcaaagccc
      841 cacaacatga ccgagatgag cttcctgaac agcgaagtgt tggcggggga cttgatgtcc
      901 cccttcgacc agtcgggttt gggggctgaa gaaagcctag gtctcttaga tgactatctg
      961 gaggtggggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1021 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1081 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1141 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1201 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1261 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1321 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1381 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1441 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1501 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1561 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1621 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1681 ctcggcatgg acgagctgta caagtaaaag ggtgggcgcg ccgacccagc tttcttgtac
     1741 aaagtggtga tatcgaattc ctgcagcccg ggggatccac tagttctaga ataacttcgt
     1801 atagcataca ttatacgaag ttattcgaca atcaacctct ggattacaaa atttgtgaaa
     1861 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1921 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1981 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2041 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2101 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2161 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2221 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     2281 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2341 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2401 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     2461 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     2521 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     2581 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2641 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2701 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     2761 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     2821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2941 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3001 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3061 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     3121 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     3181 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3241 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3301 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     3781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3901 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3961 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4021 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4081 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4141 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4201 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4261 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4321 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4381 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4441 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4501 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4561 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4621 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4681 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4741 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4801 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4861 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4921 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4981 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5041 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5101 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5161 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5221 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5281 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5341 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5401 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5461 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5521 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5581 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5641 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5701 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5761 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5821 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5881 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     5941 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttggccat tgcatacgtt
     6001 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
     6061 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6121 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6181 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     6301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     6361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     6421 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     6481 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     6541 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     6601 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgggg
     6661 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6721 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6781 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6841 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     6901 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     6961 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7021 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7081 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7141 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7201 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7261 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7321 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7381 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7441 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7501 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     7561 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     7621 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     7681 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     7741 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     7801 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     7861 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     7921 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     7981 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8041 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8101 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     8161 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     8221 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     8281 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     8341 caaaatttta tcgatcacga gactagcctc gagtgatcat aatcagccat accacatttg
     8401 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     8461 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaggca
     8521 atagcatcac aaatttcaca aataaggcat ttttttcact gcattctagt tttggtttgt
     8581 ccaaactcat caatgtatct tatcatgtct ggatctcaaa tccctcggaa gctgcgcctg
     8641 tcatcgaatt caccggtcta attaagcttg tgccccagtt tgctagggag gtcgcagtat
     8701 ctggccactg ccacctcgtg ctgctcgacg taggtctcgt tgttggcctc cttgattctt
     8761 tccagtctgt agtccacata gtagacgcca ggcatcttga ggttcttagc gggtttcttg
     8821 gatctatatg tggtcttgat gtttgcgatc agatggctcc cgcccacgag cttcagggcc
     8881 atgtcgtttc tgccttccag gccgccgtca gcggggtaca gcgtctcggt gaaggcctcc
     8941 cagccgagtg ttttcttctg catcacaggg ccgttggatg tgaagttcac ccctctgatc
     9001 ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg
     9061 cccccgtctt cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgcttg
     9121 aagaagtcgg ggatgccctg ggtgtggttg atgaaggtct tgctgccgta gaggaagcta
     9181 gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg
     9241 gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtgatggttg
     9301 tccacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcgctcatg
     9361 atggtaccga attccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
     9421 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cg
//
