LOCUS       sequence-311142-       12350 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Venus under vAChT gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Nagai Y, Nishitani N, Yasuda M, Ueda Y, Fukui Y, Andoh C, Shirakawa 
            H, Nakagawa T, Inoue KI, Nagayasu K, Kasparov S, Nakamura K, Kaneko 
            S
  TITLE     Identification of neuron-type specific promoters in monkey genome 
            and their functional validation in mice.
  JOURNAL   Biochem Biophys Res Commun. 2019 Oct 22;518(4):619-624. doi: 
            10.1016/j.bbrc.2019.08.101. Epub 2019 Aug 23.
  PUBMED    31451217
REFERENCE   2  (bases 1 to 12350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2019.08.101"; journalName: "Biochem Biophys Res 
            Commun"; date: "2019-10-22- 22"; volume: "518"; issue: "4"; pages: 
            "619-624"
COMMENT     Sequence Label: pTYF-super-mfvAChT-1.8k-Venus-WPRE
FEATURES             Location/Qualifiers
     source          1..12350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     194..215
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(289..863,864..866,867..875)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(456..473)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     683..702
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             876..1316
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1341..1883
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    1967..2555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2020..2040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2438..2449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2760..2984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3117..3136)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(4158..4175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4329..4917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4409..4428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5088..5879,5880..5948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5711..5730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5949..6053)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6121..6139
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6177..6199)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6299..6318
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7580..7600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7643..7823
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9205..9438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9700..9817
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9930..10024
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             join(11891..11893,11894..11896,11897..12350,1..260)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11936..11957)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12197..12216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
       61 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      121 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
      181 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      241 tggacgagct gtacaagtaa ggcgcgccct agtcggatat ccgtcgagcc cctctccctc
      301 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
      361 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      421 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      481 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      541 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      601 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      661 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
      721 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
      781 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      841 cctttgaaaa acacgatgat aatatggcca caaccatgaa gctactgtct tctatcgaac
      901 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
      961 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
     1021 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
     1081 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
     1141 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
     1201 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
     1261 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg
     1321 aattgggatc catggtgttt ccttctgggc agatctcaaa ccaggccctg gccttagcac
     1381 cgtcctctgc cccagtcctt gcccagacca tggtcccttc ctcagccatg gtacctctgg
     1441 ctcagccccc agctcctgcc ccagttctaa ccccgggtcc tccccagtcc ctgtctgcac
     1501 ctgttccaaa gagcacccag gctggggaag gcacgctgtc ggaagccctg ctgcacctgc
     1561 agtttgatgc tgatgaagac ttgggggcct tgcttggcaa cagcacagac ccaggagtgt
     1621 tcacagacct ggcatctgtg gacaactcag agtttcagca gctcctgaac cagggtgtgt
     1681 ccatgtctca ctccacagct gagcccatgc tgatggagta ccctgaagct ataactcgcc
     1741 tggtgacagg gtcccagagg ccccctgacc cagctcccac acccctgggg acctcggggc
     1801 ttcccaatgg tctctccgga gatgaagact tctcctccat tgcggacatg gacttctctg
     1861 ctctgctgag tcagatcagc tccagcggcc aatctagact gcagctcgac ctcgaggggg
     1921 ggcccggtac caggtaagtt aacatcgagg gatcaagctt atcgataatc aacctctgga
     1981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     2401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2521 gacgagtcgg atctcccttt gggccgcctc cccgcatcgt cgactaggga taacagggta
     2581 acggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     2641 gaaaaggggg gactggaagg gctaattcac tcccagggtc tctctggtta gaccagatct
     2701 gagcctggga gctctctggc taactaggga acccactgct taagcctcat aaagctcgac
     2761 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     2821 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     2881 gagtaggtgt cattctattc tggggggtag ggtggggcag gacagcaagg gggaggattg
     2941 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3001 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3061 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3121 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tgtgcgggag aacggagttc
     3181 tattatgact caaatcagtc tccccaagca ttcggggatc agagttttta aggataactt
     3241 agtgtgtagg gggccagtga gttggagatg aaagcgtagg gagtcgaagg tgtccttttg
     3301 cgccgagtca gttcctgggt gggggccaca agatcggatg agccagttta tcaatccggg
     3361 ggtgccagct gatccatgga gtgcagggtc tgcaaaatat ctcaagcact gattgatctt
     3421 aggttttaca atagtgatgt taccccagga acaatttggg gaaggtcaga atcttgtagc
     3481 ctgtagctgc atgactccta aaccataatt tcttttttgt tttttttttt ttatttttga
     3541 gacagggtct cactctgtca cctaggctgg agtgcagtgg tgcaatcaca gctcactgca
     3601 gcctcaacgt cgtaagctca agcgatcctc ccacctcagc ctgcctggta gctgagacta
     3661 caagcgacgc cccagttaat ttttgtattt ttggtagagg cagcgttttg ccgtgtggcc
     3721 ctggctggtc tcgaactcct gggctcaagt gatccagcct cagcctccca aagtgctggg
     3781 acaaccgggg ccagtcactg cacctggccc taaaccataa tttctaatct tttggctaat
     3841 ttgttagtcc tacaaaggca gtctagtccc caggcaaaaa gggggtttgt ttcgggaaag
     3901 ggctgttact gtctttgttt caaactataa actaagttcc tcctaaactt agttcggcct
     3961 acacccagga atgaacaagg agagcttgga ggttagaagc acgatggaat tggttaggtc
     4021 agatctcttt cactgtctga gttataattt tgcaatggtg gttcaaagac tgcccgcttc
     4081 tgacaccagt cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6061 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     6121 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6181 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6241 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     6301 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6361 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     6421 gggcgatcgg tgcgggcctc ttcgctatta cgccagggga ggcagagatt gcagtaagct
     6481 gagatcgcag cactgcactc cagcctgggc gacagagtaa gactctgtct caaaaataaa
     6541 ataaataaat caatcagata ttccaatctt ttcctttatt tatttattta ttttctattt
     6601 tggaaacaca gtccttcctt attccagaat tacacatata ttctattttt ctttatatgc
     6661 tccagttttt tttagacctt cacctgaaat gtgtgtatac aaaatctagg ccagtccagc
     6721 agagcctaaa ggtaaaaaat aaaataataa aaaataaata aaatctagct cactccttca
     6781 catcaaaatg gagatacagc tgttagcatt aaataccaaa taacccatct tgtcctcaat
     6841 aattttaagc gcctctctcc accacatcta actcctgtca aaggcatgtg ccccttccgg
     6901 gcgctctgct gtgctgccaa ccaactggca tgtggactct gcagggtccc taactgccaa
     6961 gccccacagt gtgccctgag gctgcccctt ccttctagcg gctgccccca ctcggctttg
     7021 ctttccctag tttcagttac ttgcgttcag ccaaggtctg aaactaggtg cgcacagagc
     7081 ggtaagactg cgagagaaag agaccagctt tacagggggt ttatcacagt gcaccctgac
     7141 agtcgtcagc ctcacagggg gtttatcaca ttgcaccctg acagtcgtca gcctcacagg
     7201 gggtttatca cagtgcaccc ttacaatcat tccatttgat tcacaatttt tttagtctct
     7261 actgtgccta acttgtaagt taaatttgat cagaggtgtg ttcccagagg ggaaaacagt
     7321 atatacaggg ttcagtacta tcgcatttca ggcccgagag ctgcatccgg agtatctaga
     7381 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7441 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7501 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     7561 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tactctagaa ggtctatata
     7621 agcagagctc gtttagtgaa ccgggtctct ctggttagac cagatctgag cctgggagct
     7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgctcaa
     7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7801 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagtaaag
     7861 ccagaggaga tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7921 gggcggcgac tgcagagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7981 gggtgcgaga gcgtcggtat taagcggggg agaattagat aaatgggaaa aaattcggtt
     8041 aaggccaggg ggaaagaaac aatataaact aaaacatata gtatgggcaa gcagggagct
     8101 agaacgattc gcagttaatc ctggcctttt agagacatca gaaggctgta gacaaatact
     8161 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8221 aatagcagtc ctctattgtg tgcatcaaag gatagatgta aaagacacca aggaagcctt
     8281 agataagata gaggaagagc aaaacaaaag taagaaaaag gcacagcaag cagcagctga
     8341 cacaggaaac aacagccagg tcagccaaaa ttaccctata gtgcagaacc tccaggggca
     8401 aatggtacat caggccatat cacctagaac tttaaatgca tgggtaaaag tagtagaaga
     8461 gaaggctttc agcccagaag taatacccat gttttcagca ttatcagaag gagccacccc
     8521 acaagattta aataccatgc taaacacagt ggggggacat caagcagcca tgcaaatgtt
     8581 aaaagagacc atcaatgagg aagctgcaga atgggataga ttgcatccag tgcatgcagg
     8641 gcctattgca ccaggccaga tgagagaacc aaggggaagt gacatagcag gaactactag
     8701 caaattaaga gaacaatttg gaaataataa aacaataatc tttaagcaat cctcaggagg
     8761 ggacccagaa attgtaacgc acagttttaa ttgtggaggg gaatttttct actgtaattc
     8821 aacacaactg tttaatagta cttggtttaa tagtacttgg agtactgaag ggtcaaataa
     8881 cactgaagga agtgacacaa tcacactccc atgcagaata aaacaattta taaacatgtg
     8941 gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagt ggacaaatta gatgttcatc
     9001 aaatattact gggctgctat taacaagaga tggtggtaat aacaacaatg ggtccgagat
     9061 cttcagacct ggaggaggcg atatgaggga caattggaga agtgaattat ataaatataa
     9121 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9181 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9241 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctga
     9301 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9361 actcacagtc tggggcatca aacagctcca ggcaagaatc ctggctgtgg aaagatacct
     9421 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9481 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga ataacatgac
     9541 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9601 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9661 ttgcggccgg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     9721 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     9781 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     9841 agatccactt tgggcggcct agggataaca gggtaataga tcttgctagc tcttatcatg
     9901 tctggatcca agcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc
     9961 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
    10021 cgagcggaga ctctagaggg tcccgggata cgcgcctgcg tctaatgctg ctttgctttt
    10081 gaggccggaa aaatgagaag gctcccctct gactctggaa gagagaggca aactgtaatc
    10141 tcaacaacac aacccccacc tccaacctca accaccctgg agcctctctc ccgccagtcc
    10201 gcccactgga acacggcctc catgtgccat ccagggtcaa cgccgctctg gggacgcgtc
    10261 aggcccagcg cacagcctgg gcagctcggc ctgccagctg agcacgggtg cctcaagggg
    10321 tgcggccctc ccagaaggaa ggtgagtctc cctggagcct ctgacgacag cgaagtggct
    10381 gggcgctctc cggggagtag gcgcggcacc caagaagggt ttcttacagt tccaagagag
    10441 gaccccagcc acagtcactc cagagcccac ccggggcttg gaggaggctc tggaaaagga
    10501 gggatgggga gtgtgcagaa cagggggcgg aggctggcag gagccgaggg agggctcggt
    10561 cgggtgcgaa gagtgcctgg gaggagctag agtggggagg gggtgcggtg gcggggagag
    10621 aagaggcgga gctgggggac ccgaccgcta aagcggaaag ggggattcca ggcaggggga
    10681 gtgtggccag gcagtgcgag agggcggcag cggccaagct gatattcgcg accccgggtg
    10741 ggggcgaggc aacttctgcc ctcagtcccc acaagctccc ggccgggatt cgctgcagag
    10801 aaattctgca gaggggccgg tgggtcagag ccaggccagg cagttgctga gccttcccct
    10861 cccctcccgg cggggtgtgt gtgcaagggg ccagggggcg gagtcggaga aagggttggg
    10921 ggtgtccgtg tccagcgctc ctccggatgc cgctccacaa tccagctgcg aaaacagatg
    10981 gacgcagcgg cggctcacct cgggggcttc ctgcccgctg tgcgccgagg tccaggctga
    11041 ggcggagatc gcaagcctcc ggctcccgcc tctctcgagg ggccgcgagg aacaggctgc
    11101 cgcgcctccc tccccaggag aagagtgggc gctgaccccg cgggtcctag aaccgcgggc
    11161 ggggggcggg cagggggagg gcagagggtc ggctccgggc gcggaacacc catcccaggg
    11221 gctggttcag gacttccgca ccgagacccc ttcctgggcc ttcagccctg gagcatctgg
    11281 ggagcggacc cctgtcctgc cacgccccgc ccccggcccc cgccccgccg acgtcgcatt
    11341 agcatgagcg acgcaagtgg cccgggcacc actcgggggc tgggactcgc cgcgtcacag
    11401 ccccgagtgg aagggaaaaa aaaaagagga ggcggcggag gaggcaagag ccgacgcgag
    11461 gggaggggag cgcggcggcg gggctaacgg gcgggcaacc gggcgggcgg caacagcatg
    11521 cccctcggcc agcgcgggcg gcctctcagc gcggtggggg ctgctctggg cgcgctcagg
    11581 gcacagtgcg cccagtctcc ggccccggcc cctcgtcgcg cccgccttcc cggccgcccc
    11641 tgagcccagc agccgcgggt cccgggaacc gctaagagta gcggcaacgc ctagccggac
    11701 agagtccttt cctttgccgg gacgctgggc catgagcccc gcggccacct gaggcacggg
    11761 gggtctgctc ggccaggacc gcctccccga agtaccgtgc cctcgcctct gcactgcggg
    11821 acgccagcgc tcggccctgg cggaggcgtc ctcggaagag catcggggtg ggggcggccg
    11881 cggatccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
    11941 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
    12001 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
    12061 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
    12121 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
    12181 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
    12241 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
    12301 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
//
