LOCUS       sequence-311550-       11658 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For PiggyBac-mediated integration of V5-tagged siRNA-resistant 
            full-length CASC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Gerbracht JV, Boehm V, Britto-Borges T, Kallabis S, Wiederstein JL, 
            Ciriello S, Aschemeier DU, Kruger M, Frese CK, Altmuller J, 
            Dieterich C, Gehring NH
  TITLE     CASC3 promotes transcriptome-wide activation of nonsense-mediated 
            decay by the exon junction complex.
  JOURNAL   Nucleic Acids Res. 2020 Jul 4. pii: 5867419. doi: 
            10.1093/nar/gkaa564.
  PUBMED    32621609
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Jul 4. pii: 5867419. doi: 10.1093/nar/gkaa564."
COMMENT     Sequence Label: PB-CuO-V5 CASC3 siRes1+2 f.l. WT
FEATURES             Location/Qualifiers
     source          1..11658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             1200..1241
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(3375..3949,3950..3952,3953..3961)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3542..3559)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3769..3788
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3998..4660
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        4781..4992
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5005..5273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5301..5310
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5313..5339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5319..5345
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5457..6065
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             6102..6155
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6162..6761
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6162..6181)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6658..6678
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6808..6929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6845..6864)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6899..6918
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7692..7726
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8217..8233)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8217..8233)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8230..8252)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8241..8257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8265..8271,8272..8289,8290..8295))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8310..8331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8448..8465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8619..9207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8699..8718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9378..10169,10170..10238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10001..10020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10239..10343)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10369..10824)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10506..10527)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10718..10737
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10951..10973
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10965..10982
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10966..10982
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   11112..11174
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta atacccccgc cccgttgacg caaatgggca agcttgccgg
      481 gtcgaggtag gcgtgtacgg tgggaggcct atataagcaa ccggtataat caaacagacc
      541 agattgtctg tttgttaccg gtgtttagtg aaccgggcgc gcctcatatc gcctggagac
      601 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggtc
      661 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac
      721 tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtactcc
      781 gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc
      841 gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg
      901 gtcggggttg tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag
      961 acggcggatg gtcgaggtga ggtgtggcag gcttgagatc cagctgttgg ggtgagtact
     1021 ccctctcaaa agcgggcatt acttctgcgc taagattgtc agtttccaaa aacgaggagg
     1081 atttgatatt cacctggccc gatctggcca tacacttgag tgacaatgac atccactttg
     1141 cctttctctc cacaggtgtc cactcccagg tccaagtttg gtctagagct agcaccatgg
     1201 gcaagcccat ccctaaccca ctgctgggcc tggacagcac cccacctctc gagatggcgg
     1261 accggcggcg gcagcgcgct tcgcaagaca ccgaggacga ggaatctggt gcttcgggct
     1321 ccgacagcgg cggctccccg ttgcggggag gcgggagctg cagcggtagc gccggaggcg
     1381 gcggcagcgg ctctctgcct tcacagcgcg gaggccgaac cggggccctt catctgcggc
     1441 gggtggagag cgggggcgcc aagagtgctg aggagtcgga gtgtgagagt gaagatggca
     1501 ttgaaggtga tgctgttctc tcggattatg aaagtgcaga agactcggaa ggtgaagaag
     1561 gtgaatacag tgaagaggaa aactccaaag tggagctgaa atcagaagct aatgatgctg
     1621 ttaattcttc aacaaaagaa gagaagggag aagaaaagcc tgacaccaaa agcactgtga
     1681 ctggagagag gcaaagtggg gacggacagg agagcacaga gcctgtggag aacaaagtgg
     1741 gtaaaaaggg ccctaagcat ttggatgatg atgaagatcg gaagaatcca gcatacatac
     1801 ctcggaaagg gctcttcttt gagcatgatc ttcgagggca aactcaggag gaggaagtca
     1861 gacccaaggg gcgtcagcga aagctatgga aggatgaggg tcgctgggag catgacaagt
     1921 tccgggaaga tgagcaggcc ccaaagtccc gacaggagct cattgctctt tatggttatg
     1981 acattcgctc agctcataat ccagacgata ttaagccaag gcggatccgg aaaccccgat
     2041 atgggagtcc tccacaaaga gatccaaact ggaacggtga gcggctaaac aagtctcatc
     2101 gccaccaggg tcttgggggc accctaccac caaggacatt tattaacagg aatgctgcag
     2161 gtaccggccg tatgtctgca cccaggaatt attcccgatc tgggggcttc aaggaaggtc
     2221 gtgctggttt taggcctgtg gaagctggtg ggcagcatgg tggccggtct ggtgagactg
     2281 ttaagcatga gattagttac cggtcacggc gcctagagca gacttctgtg agggatccat
     2341 ctccagaagc agatgctcca gtgcttggca gtcctgagaa ggaagaggca gcctcagagc
     2401 caccagctgc tgctcctgat gctgcaccac caccccctga taggcccatt gagaagaaat
     2461 cctattcccg ggcaagaaga actcgaacca aagttggaga tgcagtcaag cttgcagagg
     2521 aggtgccccc tcctcctgaa ggactgattc cagcacctcc agtcccagaa accaccccaa
     2581 ctccacctac taagactggg acctgggaag ctccggtgga ttctagtaca agtggacttg
     2641 agcaagatgt ggcacaacta aatatagcag aacagaattg gagtccgggg cagccttctt
     2701 tcctgcaacc acgggaactt cgaggtatgc ccaaccatat acacatggga gcaggacctc
     2761 cacctcagtt taaccggatg gaagaaatgg gtgtccaggg tggtcgagcc aaacgctatt
     2821 catcccagcg gcaaagacct gtgccagagc cccccgcccc tccagtgcat atcagtatca
     2881 tggagggaca ttactatgat ccactgcagt tccagggacc aatctatacc catggtgaca
     2941 gccctgcccc gctgcctcca cagggcatgc ttgtgcagcc aggaatgaac cttccccacc
     3001 caggtttaca tccccaccag acaccagctc ctctgcccaa tccaggcctc tatcccccac
     3061 cagtgtccat gtctccagga cagccaccac ctcagcagtt gcttgctcct acttactttt
     3121 ctgctccagg cgtcatgaat ttcggcaacc caagttaccc ttatgctcca ggggcactgc
     3181 ctcccccacc accgcctcat ctgtatccta atacacaggc cccatcacag gtatatggag
     3241 gagtgaccta ctataacccc gcccagcagc aggtgcagcc aaagccctcc ccaccccgga
     3301 ggactcccca gccagtcacc atcaagcccc ctccacctga ggttgtaagc aggggttcca
     3361 gttaagcggc cgcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     3421 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     3481 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     3541 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     3601 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     3661 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     3721 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     3781 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     3841 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     3901 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     3961 catggcgtcc ggaatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg
     4021 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
     4081 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
     4141 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
     4201 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
     4261 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
     4321 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
     4381 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
     4441 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
     4501 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
     4561 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
     4621 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc
     4681 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgct agagctgaat
     4741 ctaagtcgac ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     4801 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     4861 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     4921 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     4981 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5041 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5101 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5161 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5221 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5281 tgaccggcgc ctactctaga gccgccatgg cttacccata cgatgttcca gattacgcta
     5341 gtttggtgag gcagcagaga ccgatgacat ccactttgcc tttctctcca caggtgtcca
     5401 ctcccaggtc caagtttgga agatctacca tgccaaagag acccagaccc gtgatcatga
     5461 gtccaaagag aagaacacag gcagagcgcg caatggagac ccagggcaag ttgattgcag
     5521 cggccctggg ggttttacgg gaaaaaggtt acgcgggatt ccggatcgca gatgtgcccg
     5581 gtgctgcagg tgtctcgaga ggagcgcaga gccatcattt cccgacaaag cttgagcttc
     5641 tgcttgccac ttttgaatgg ctttacgaac agatcaccga acgcagtcgg gctcgattag
     5701 cgaaattgaa gccagaggat gacgtcatcc agcaaatgct ggacgacgcc gccgaatttt
     5761 tcctcgacga tgacttctct atcagccttg atttgattgt ggctgccgac cgggatccag
     5821 cgttacgcga gggtattcag cgcacggtag agaggaatcg gtttgtcgtc gaggatatgt
     5881 ggcttggtgt tctggtgagc cgtggtcttt cgcgtgatga tgcagaagat atcctttggt
     5941 tgatattcaa ttcggtgcgt gggcttgctg ttcgtagcct atggcagaag gacaaagaac
     6001 gctttgagcg tgtcaggaac tcgacactcg aaattgcgcg agagcggtac gcgaaattca
     6061 agcgcgcggc caaaccctct ggccggtctc tctctgttcg agagggcaga ggaagtcttc
     6121 taacatgcgg tgacgtggag gagaatcccg gccctgaatt catgaccgag tacaagccca
     6181 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6241 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6301 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6361 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6421 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6481 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6541 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6601 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6661 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6721 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg
     6781 attacaaaat ttgtggttaa ctaactaaac ttgtttattg cagcttataa tggttacaaa
     6841 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6901 ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa
     6961 ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata
     7021 tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg
     7081 cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag
     7141 ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc
     7201 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     7261 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     7321 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     7381 attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca agaaaatata
     7441 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     7501 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     7561 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     7621 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     7681 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca
     7741 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     7801 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     7861 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     7921 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     7981 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     8041 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     8101 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     8161 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca
     8221 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8281 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8341 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9481 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9601 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9661 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9721 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9781 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9841 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9901 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9961 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10021 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10081 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10141 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10201 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
    10381 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    10441 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    10501 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    10561 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    10621 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    10681 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    10741 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    10801 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
    10861 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10921 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10981 gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg
    11041 caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc
    11101 agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgtgt
    11161 aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag
    11221 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat
    11281 ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
    11341 aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
    11401 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
    11461 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
    11521 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
    11581 aggcctccac ggccagacta gttattaata gtaatcaatt acggggtcat tagttcatag
    11641 cccatatatg gagttccg
//
