LOCUS       sequence-311558-        8743 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector expressing GFP driven by human EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Au YZ, Gu M, De Braekeleer E, Gozdecka M, Aspris D, Tarumoto Y, 
            Cooper J, Yu J, Ong SH, Chen X, Tzelepis K, Huntly BJP, Vassiliou G,
            Yusa K
  TITLE     KAT7 is a genetic vulnerability of acute myeloid leukemias driven by
            MLL rearrangements.
  JOURNAL   Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. 
            Epub 2020 Aug 6.
  PUBMED    32764680
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-020-1001-z"; journalName: "Leukemia"; date: 
            "2021-04"; volume: "35"; issue: "4"; pages: "1012-1022"
COMMENT     Sequence Label: pKLV-EF1aGFP-W
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..35
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        105..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        410..608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             626..806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    853..978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1475..1708
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1893..1937
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2235..2352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2823..4004
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3054..3996
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3953..3973
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4017..4026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4023..4025,4026..4028,4029..4742)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4023..4025,4026..4028,4029..4739)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4068..4089)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4329..4348)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4676..4697
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4808..4828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5226..5237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5689..5706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5695..5919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5945..5965)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5945..5963)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5984..6000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5984..6000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5997..6019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6008..6024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6032..6038,6039..6056,6057..6062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6077..6098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6215..6232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6386..6974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6466..6485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7145..7936,7937..8005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7768..7787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8006..8110)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8136..8591)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8273..8294)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8485..8504
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8718..8740
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(8732..8743,1..6)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(8733..8743,1..6)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      781 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      841 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      901 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      961 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1021 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1081 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1141 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1201 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1261 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1321 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1381 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1441 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1501 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1561 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1621 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1681 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1741 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1801 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1861 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1921 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1981 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     2041 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2101 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2161 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     2221 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     2281 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2341 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc
     2401 cctacgcgtg aggggcgcgg aagccggggc ggaggaggag ccaaccccgg gctgggctga
     2461 gacccgcaga ggaagacgct ctagggattt gtcccggact agcgagatgg caaggctgag
     2521 gacgggaggc tgattgagag gcgaaggtac accctaatct caatacaacc tttggagcta
     2581 agccagcaat ggtagaggga agattctgca cgtcccttcc aggcggcctc cccgtcacca
     2641 ccccccccaa cccgccccga ccggagctga gagtaattca tacaaaagga ctcgcccctg
     2701 ccttggggaa tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct
     2761 gcgttcccgc cccctcaccc gcccgctctc gtcatcactg aggtggagaa gagcatgcgt
     2821 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2881 ggggaggggt cggcaattga accggtgcct agagaaagtg gcgcggggta aactgggaaa
     2941 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     3001 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     3061 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     3121 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     3181 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     3241 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     3301 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     3361 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     3421 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     3481 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     3541 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3601 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3661 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3721 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3781 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3841 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3901 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3961 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtggaagct tggcgcgcca
     4021 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4081 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4141 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4201 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4261 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4321 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4381 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4441 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4501 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4561 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4621 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4681 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4741 aagaattcgc ggccgctcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5161 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5341 cgcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5401 aagaaaaggg gggactggaa gggctaattc actcccaaag aagtcaagat ctgctttttg
     5461 cctgtactgg gtctctctgg ttagaccaga gtctctctgg ttagaccaga tctgagcctg
     5521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5641 cttttagtca gtgtggaaaa tctctagcag tttaaacccg ctgatcagcc tcgactgtgc
     5701 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5761 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5821 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5881 tcaatagcag gcatgctggg gatgcggtgg gctctatggg cggccgttaa ttaagctttt
     5941 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     6001 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta
     6061 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6121 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7141 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7201 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7261 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7321 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7381 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7441 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7501 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7561 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7621 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7681 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7741 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7801 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7861 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7921 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7981 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8041 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8101 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     8161 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8221 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8281 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8341 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8401 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8461 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8521 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8581 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     8641 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     8701 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acg
//
