LOCUS       sequence-311576-       10943 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1950..1970
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1970..3181
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3018..3037
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3182..3206
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4704..4728)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4730..4771
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4778..5548
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(5554..5574)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5603..6191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5656..5676)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6074..6085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6205..6704
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6240..6263)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6637..6656
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6725..7324
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6725..6744)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7221..7241
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7462..7695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7767..7888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7804..7823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7858..7877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7928..8063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8083..8102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8084..8102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8112..8129)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8112..8128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8121..8143)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8270..8725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8357..8376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8567..8588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8751..8855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8856..8924,8925..9716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9887..10475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10376..10395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10629..10646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10763..10784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10799..10804,10805..10822,10823..10829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10837..10853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10842..10864
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10861..10877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10861..10877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10896..10916
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10898..10916
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgtccagtgt ggtggaattc tgcagatatc aactttgtat agaaaagttg gcctccgcgc
     1981 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     2041 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     2101 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     2161 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     2221 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     2281 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     2341 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     2401 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     2461 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     2521 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     2581 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     2641 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     2701 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     2761 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     2821 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     2881 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     2941 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     3001 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     3061 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     3121 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     3181 cgcaactttg tacaaaaaag ttggcaccat gctagaacgg cctcctgcac tggccatgcc
     3241 catgcccacg gagggcaccc cgccacctct gagtggcacc cccatcccag tcccagccta
     3301 cttccgccac gcagaacctg gattctccct caagaggccc agggggctca gccggagcct
     3361 cccacctccg ccccctgcca agggcagcat tcccatcagc cgcctcttcc ctcctcggac
     3421 cccaggctgg caccagctgc agccccggcg ggtgtcattc cggggcgagg cctcagagac
     3481 tctgcagagc cctgggtatg acccaagccg gccagagtcc ttcttccagc agagcttcca
     3541 gaggctcagc cgcctgggcc atggctccta cggagaggtc ttcaaggtgc gctccaagga
     3601 ggacggccgg ctctatgcgg taaagcgttc catgtcacca ttccggggcc ccaaggaccg
     3661 ggcccgcaag ttggccgagg tgggcagcca cgagaaggtg gggcagcacc catgctgcgt
     3721 gcggctggag caggcctggg aggagggcgg catcctgtac ctgcagacgg agctgtgcgg
     3781 gcccagcctg cagcaacact gtgaggcctg gggtgccagc ctgcctgagg cccaggtctg
     3841 gggctacctg cgggacacgc tgcttgccct ggcccatctg cacagccagg gcctggtgca
     3901 ccttgatgtc aagcctgcca acatcttcct ggggccccgg ggccgctgca agctgggtga
     3961 cttcggactg ctggtggagc tgggtacagc aggagctggt gaggtccagg agggagaccc
     4021 ccgctacatg gcccccgagc tgctgcaggg ctcctatggg acagcagcgg atgtgttcag
     4081 tctgggcctc accatcctgg aagtggcatg caacatggag ctgccccacg gtggggaggg
     4141 ctggcagcag ctgcgccagg gctacctgcc ccctgagttc actgccggtc tgtcttccga
     4201 gctgcgttct gtccttgtca tgatgctgga gccagacccc aagctgcggg ccacggccga
     4261 ggccctgctg gcactgcctg tgttgaggca gccgcgggcc tggggtgtgc tgtggtgcat
     4321 ggcagcggag gccctgagcc gagggtgggc cctgtggcag gccctgcttg ccctgctctg
     4381 ctggctctgg catgggctgg ctcaccctgc cagctggcta cagcccctgg gcccgccagc
     4441 caccccgcct ggctcaccac cctgcagttt gctcctggac agcagcctct ccagcaactg
     4501 ggatgacgac agcctagggc cttcactctc ccctgaggct gtcctggccc ggactgtggg
     4561 gagcacctcc accccccgga gcaggtgcac acccagggat gccctggacc taagtgacat
     4621 caactcagag cctcctcggg gctccttccc ctcctttgag cctcggaacc tcctcagcct
     4681 gtttgaggac accctagacc caaccccaac tttcttgtac aaagtgggcg gcaagcccat
     4741 ccccaacccc ctgctgggcc tggacagcac cgctagcatc ccgctgctga acgctaaaca
     4801 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     4861 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     4921 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     4981 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     5041 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     5101 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     5161 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     5221 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     5281 cctggacagg aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact
     5341 gttcgagcag gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat
     5401 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     5461 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     5521 agaaatcagt ctgcggtctg ccgaaaagta agccaacttt attatacata gttgatatcc
     5581 agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     5641 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5701 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5761 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5821 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5881 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5941 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6001 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6061 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6121 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6181 cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     6241 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     6301 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     6361 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     6421 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6481 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6541 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6601 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6661 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     6721 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6781 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6841 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6901 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6961 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7021 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7081 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7141 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7201 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7261 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7321 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     7381 tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
     7501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7681 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7741 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7861 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7921 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7981 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8041 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8101 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8161 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8221 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8701 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8761 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8821 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8881 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8941 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9001 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9061 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9361 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9421 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9481 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9541 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9601 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9661 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9721 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9781 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10021 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10681 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10741 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10801 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10861 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    10921 aaaagctgga gctgcaagct taa
//
