LOCUS       sequence-31166-e         770 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Johnson RC, Penzes P, Eipper BA, Mains RE
  TITLE     Isoforms of kalirin, a neuronal Dbl family member, generated through
            use of different 5'- and 3'-ends along with an internal 
            translational initiation site.
  JOURNAL   J Biol Chem. 2000 Jun 23. 275(25):19324-33.
  PUBMED    10777487
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jun 23. 275(25):19324-33."
COMMENT     Sequence Label: pEAK10-His-Myc-Kal9
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..69
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cttgccacca tggcccatca ccatcaccat cacaacatgg agcaaaagct catttctgag
       61 gaagatctca atggtggtgg tggtgggtcg acaggatctt ttcggaatga tggtttgaaa
      121 gcttctgatg tccttcctat cctaaaggag aaagtggcct ttgtgtctgg gggtcgtgat
      181 aagcgaggtg gacccatcct gaccttccct gcccgaagca atcatgacag aataagacag
      241 gaagacctac ggaaactcgt gacgtatttg gccagcgtgc caagcgagga cgtttgcaaa
      301 cgtggcttca ccgtcatcat cgacatgcgg ggctccaagt gggacctcat caagcccctc
      361 ctcaagacgc tgcaggaagc ctttcccgcc gagatccatg tggccctcat catcaagcct
      421 gacaacttct ggcagaagca gaagaccaac tttggcagct ccaaattcat ctttgagacg
      481 agcatggtgt ctgtggaggg cctcacgaag ctggtggacc cctcccagct gacagaagag
      541 tttgacggtt ccctggatta caaccacgag gagtggattg agttgcgcct ctccctggag
      601 gagttcttca acagtgcggt ccacctgctc tcacgcctcg aggacctgca ggagatgctg
      661 gcccggaagg agttccctgt ggatgtggag ggctctcgga ggcttatcga cgagcacaca
      721 caacttaaga aaaaggtgct gaaggccccc gtggaagagc tggaccggga
//
