LOCUS       sequence-31168-e         819 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Penzes P, Johnson RC, Alam MR, Kambampati V, Mains RE, Eipper BA
  TITLE     An isoform of kalirin, a brain-specific GDP/GTP exchange factor, is 
            enriched in the postsynaptic density fraction.
  JOURNAL   J Biol Chem. 2000 Mar 3. 275(9):6395-403.
  PUBMED    10692441
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 3. 275(9):6395-403."
COMMENT     Sequence Label: pEAK10-His-Myc-Kal7
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..37
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..73
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaagcttgcc accatggccc atcaccatca ccatcacaac atggagcaaa agctcatttc
       61 tgaggaagat ctcaatggtg gtggtggtgg gtcgacagga tcttttcgga atgatggttt
      121 gaaagcttct gatgtccttc ctatcctaaa ggagaaagtg gcctttgtgt ctgggggtcg
      181 tgataagcga ggtggaccca tcctgacctt ccctgcccga agcaatcatg acagaataag
      241 acaggaagac ctacggaaac tcgtgacgta tttggccagc gtgccaagcg aggacgtttg
      301 caaacgtggc ttcaccgtca tcatcgacat gcggggctcc aagtgggacc tcatcaagcc
      361 cctcctcaag acgctgcagg aagcctttcc cgccgagatc catgtggccc tcatcatcaa
      421 gcctgacaac ttctggcaga agcagaagac caactttggc agctccaaat tcatctttga
      481 gacgagcatg gtgtctgtgg agggcctcac gaagctggtg gacccctccc agctgacaga
      541 agagtttgac ggttccctgg attacaacca cgaggagtgg attgagttgc gcctctccct
      601 ggaggagttc ttcaacagtg cggtccacct gctctcacgc ctcgaggacc tgcaggagat
      661 gctggcccgg aaggagttcc ctgtggatgt ggagggctct cggaggctta tcgacgagca
      721 cacacaactt aagaaaaagg tgctgaaggc ccccgtggaa gagctggacc gggaggggca
      781 gcggctactg cagtgtatcc gctgcagtga tggcttctc
//
