LOCUS       sequence-311898-        1085 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Express a fragment of yeast pol1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL,
            Xu RM, Zhang Z
  TITLE     DNA polymerase alpha interacts with H3-H4 and facilitates the 
            transfer of parental histones to lagging strands.
  JOURNAL   Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. 
            eCollection 2020 Aug.
  PUBMED    32923642
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-08-26"; volume: "6(35):eabb5820. doi"; pages: " 
            10.1126/sciadv.abb5820. eCollection 2020 Aug"
COMMENT     Sequence Label: pGST-pol1-N1-2A2
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..62
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(546..568)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(713..731)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        799..903
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtttggtgg tggcgnccat cctccnaaaa tcgatacgac cgaaaacctg tattttcagg
       61 gcgccatgga tccgggatcc catgtcttct aaaagtgaaa aattagaaaa attgagaaag
      121 ctgcaagccg ctcgaaatgg tacatccatc gatgattacg aaggtgacga atcagatggt
      181 gacagaatat acgacgaaat cgacgaaaaa gaatacaggg ccagaaagcg ccaggaattg
      241 ctgcatgacg atgctgtagt tgacgccgat ggtgtaggct acgtcgatcg tggtgtcgaa
      301 gaagattgga gggaagttga taacagttca agtgatgaag acactggaaa ccttgctagc
      361 aaggattcta aaaggaagaa gaatataaaa agggagaaag accaccagat ttgaggaatt
      421 caaaggccta cgtcgacgag ctcaactagt gcggccgctt tcgaatctag agcctgcagt
      481 ctcgagcggc cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt
      541 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
      601 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
      661 atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg
      721 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      901 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      961 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttggtg
     1021 ccccnaagtg ggttacatcg aactggatct caacagcggn aagaatcctt gaaangtttt
     1081 tggcc
//
