LOCUS       sequence-31213-e         902 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPI64
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..93
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             660..677
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(730..748)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      744..791
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
       61 catcacagca gcggcctggt tccgcgtggt agtatgggaa taaaggttca acgtcctcga
      121 tgtttttttg acattgccat taacaatcaa cctgctggaa gagttgtctt tgaattattt
      181 tctgatgtgt gccccaaaac atgcgagaac tttcgttgtc tttgtacagg tgaaaagggg
      241 accgggaaat caactcagaa accattacat tataagagtt gtctctttca cagagttgtc
      301 aaggatttta tggttcaagg tggtgacttc agtgaaggaa atggacgagg aggggaatct
      361 atctatggag gattttttga agacgagagt ttcgctgtta aacacaacaa agaatttctc
      421 ttgtcaatgg ccaacagagg gaaggataca aatggttcac agttcttcat aacaacgaaa
      481 ccaactcctc atttagatgg gcatcatgtt gtttttggac aagtaatctc tggtcaagaa
      541 gttgtaagag agattgaaaa ccagaaaaca gatgcagcta gcaaaccgtt tgcggaggta
      601 cggatactca gttgtggaga gctgattccc tgatgacgaa gcttgcggcc gcactcgagc
      661 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      721 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      781 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      841 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      901 cg
//
