LOCUS       sequence-31250-e         928 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IQGAP2
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             412..429
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(482..500)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      496..543
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(667..686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     877..898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca
       61 cagcagcggc agagaaaact tgtatttcca gggcgctaaa ccagttaaat ataccgccgc
      121 gaaactgcac gaaaaaggcg tactgctgga catcgacgat ctgcagacca atcagtttaa
      181 aaatgtgacc tttgacatca tcgctacgga agatgtcggt atcttcgacg ttcgttctaa
      241 atttctggga gttgaaatgg agaaggtgca gctgaacatc caggacctgc tgcaaatgca
      301 atatgaaggt gtcgcagtaa tgaagatgtt tgacaaagtg aaggtgaatg tgaacctgct
      361 gatttacctg ctgaacaaaa aatgatgacg aagcttgcgg ccgcactcga gcaccaccac
      421 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      481 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      541 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      601 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      661 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      721 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      781 ccaaaaanct tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      841 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      901 caacactcaa ccctatctcg gtctattc
//
