LOCUS       sequence-31267-e         857 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PLXND1 (3H6N)
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             432..449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(502..520)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      516..563
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(687..706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc gcaaaaccac gtaatctgaa cgttagcttt
      121 caaggttgtg gcatggattc gctgtcggtt cgcgctatgg acacagatac cctgacgcag
      181 gtcaaagaaa aaatcctgga agcgttctgc aaaaatgttc cttatagtca gtggccgcgt
      241 gccgaggacg tagatctgga atggtttgca tcaagcaccc aatcctatat tctgcgcgac
      301 ctggatgata ctagcgtggt tgaggacggt cgtaaaaaac tgaacacact ggcgcactac
      361 aaaattccgg aaggggcttc tctggccatg agtttaatcg attgatgacg aagcttgcgg
      421 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      481 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      541 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc ccttttaggg ttccgattta
      781 gtgctttacg gcaccctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      841 ccatcgccct gatagac
//
