LOCUS       sequence-312907-        9721 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fluorescent reporter for intestinal stem cells through ASCL2 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Heinz MC, Oost KC, Snippert HJG
  TITLE     Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal 
            Organoids.
  JOURNAL   STAR Protoc. 2020 Oct 8;1(3):100126. doi: 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
  PUBMED    33377020
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-10-8"; volume: "1(3):100126. doi"; pages: " 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18"
COMMENT     Sequence Label: pLV 4xSTAR-sTomato-NLS-blast
FEATURES             Location/Qualifiers
     source          1..9721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2348..2368
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2350..2368
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2396..2622
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2623..2803
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2850..2975
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3468..3701
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3886..3930
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4192..4309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(4826..4844)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5094..5113
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5288..5311
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             5407..5427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5477..6029
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5621..5638)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5848..5867
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      6050..6059
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6056..6454
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7297..7530
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7602..7723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7639..7658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7693..7712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7763..7898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7918..7937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7919..7937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7947..7964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7947..7963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7956..7978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8105..8560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8192..8211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8402..8423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8586..8690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8691..8759,8760..9551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8909..8928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgct gatgaatccc
     1021 ctaatgattt tggtaaaaat cattaagtta aggtggatac acatcttgtc atatgatcaa
     1081 atggtttcgc gaaaaatcaa taatcagaca acaagatgtg cgaactcgat attttacacg
     1141 actctcttta ccaattctgc cccgaattac acttaaaacg actcaacagc ttaacgttgg
     1201 cttgccacgc attacttgac tgtaaaactc tcactcttac cgaacttggc cgtaacctgc
     1261 caaccaaagc gagaacaaaa cataacatca aacgaatcga ccgattgtta ggtaatcgtc
     1321 acctccacaa agagcgactc gctgtatacc gttggcatgc tagctttatc tgttcgggca
     1381 atacgatgcc cattgtactt gttgactggt ctgatattcg tgagcaaaaa cgacttatgg
     1441 tattgcgagc ttcagtcgca ctacacggtc gttctgttac tctttatgag aaagcgttcc
     1501 cgctttcaga gcaatgttca aagaaagctc atgaccaatt tctagccgac cttgcgagca
     1561 ttctaccgag taacaccaca ccgctcattg tcagtgatgc tggctttaaa gtgccatggt
     1621 ataaatccgt tgagaagctg ggttggtact ggttaagtcg agtaagagga aaagtacaat
     1681 atgcagacct aggagcggaa aactggaaac ctatcagcaa cttacatgat atgtcatcta
     1741 gtcactcaaa gactttaggc tataagaggc tgactaaaag caatccaatc tcatgccaaa
     1801 ttctattgta taaatctcgc tctaaaggcc gaaaaaatca gcgctcgaca cggactcatt
     1861 gtcaccaccc gtcacctaaa atctactcag cgtcggcaaa ggagccatgg gttctagcaa
     1921 ctaacttacc tgttgaaatt cgaacaccca aacaacttgt taatatctat tcgaagcgaa
     1981 tgcagattga agaaaccttc cgagacttga aaagtcctgc ctacggacta ggcctacgcc
     2041 atagccgaac gagcagctca gagcgttttg atatcatgct gctaatcgcc ctgatgcttc
     2101 aactaacatg ttggcttgcg ggcgttcatg ctcagaaaca aggttgggac aagcacttcc
     2161 aggctaacac agtcagaaat cgaaacgtac tctcaacagt tcgcttaggc atggaagttt
     2221 tgcggcattc tggctacaca ataacaaggg aagacttact cgtggctgca accctactag
     2281 ctcaaaattt attcacacat ggttacgctt tggggaaatt atgaggggat ctctcagcgc
     2341 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     2401 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     2461 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     2521 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     2581 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     2641 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2701 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2761 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2821 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2881 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2941 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     3001 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     3061 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     3121 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3181 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3241 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3301 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3361 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3421 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3481 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3541 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3601 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3661 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3721 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3781 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3841 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3901 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3961 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4021 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4081 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4141 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttttaaaag
     4201 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4261 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcggta
     4321 cccctagggt gaaaaggcag gtgcaaagca aatcctatcc cagcttctag ctaggtgaaa
     4381 aggcaggtgc aaagcaaatc ctatcccagc ttctagctag ggtgaaaagg caggtgcaaa
     4441 gcaaatccta tcccagcttc tagctaggtg aaaaggcagg tgcaaagcaa atcctatccc
     4501 agcttctagc tagcggcgcg ccggatccgg aggggacaag tggagggaaa gtggggccgg
     4561 gcggggggtg cctgggaagc caggctgcgc tgacgtcact gggcgcgcaa ttcgggctgg
     4621 agcgctttaa aaaacgagcg tgcaagcaga gaggctgctc cacaccgctc aggccgcggt
     4681 ttaaacatcg atatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     4741 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4801 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     4861 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     4921 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     4981 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     5041 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     5101 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     5161 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     5221 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     5281 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     5341 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
     5401 tccaggccaa aaaagaaaag aaaggtcatc gattagttaa ttaatggccc gggaattccg
     5461 cccctctccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5521 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5581 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5641 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5701 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5761 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5821 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5881 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5941 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6001 gttttccttt gaaaaacacg atgataatat ggatatggcc acaacggtcg ccaccatggc
     6061 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     6121 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     6181 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     6241 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     6301 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     6361 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     6421 attgctgccc tctggttatg tgtgggaggg ctaaatgcat gtattcaatc taagcaggct
     6481 ttcactttct cgccaactta caaggccttt ctgtgtaaac aatacctgaa cctttacccc
     6541 gttgcccggc aacggccagg tctgtgccaa gtgtttgctg acgcaacccc cactggctgg
     6601 ggcttggtca tgggccatca gcgcatgcgt ggaacctttt cggctcctct gccgatccat
     6661 actgcggaac tcctagccgc ttgttttgct cgcagcaggt ctggagcaaa cattatcggg
     6721 actgataact ctgttgtcct atcccgcaaa tatacatcgt ttccatggct gctaggctgt
     6781 gctgccaact ggatcctgcg cgggacgtcc tttgtttacg tcccgtcggc gctgaatcct
     6841 gcggacgacc cttctcgggg tcgcttggga ctctctcgtc cccttctccg tctgccgttc
     6901 cgaccgacca cggggcgcac ctctctttac gcggactccc cgtctgtgcc ttctcatctg
     6961 ccggaccgtg tgcacttcgc ttcacctctg cacgtcgcat ggagaccacc gtgaacgccc
     7021 accaaatatt gcccaaggtc ttacataaga ggactcttgg actctcagca atgtcaacga
     7081 ccgaccttga ggcatacttc aaagactgtt tgtttaaaga ctgggaggag ttgggggagg
     7141 agattaggtt aaaggtcttt gtactaggag gctgtaggca taaattggtc tgcgcaccag
     7201 caccatggcg caatcactag agcggggtac ctttaagacc aatgacttac aaggcagctg
     7261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7501 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7561 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7621 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7681 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7741 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7861 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7921 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     8101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9721 c
//
