LOCUS       sequence-313014-         519 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Constitutive expression of intC-ZF10-NLS-HA under the EF1-alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM522 EF1-alpha intC-ZF10-NLS-HA in TUPV2
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             457..477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             490..516
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgatgctga agaagatcct gaagatcgag gagctggacg agagagagct gatcgacatc
       61 gaggtgagcg gcaaccacct gttctacgcc aacgacatcc tgacccacaa cagcggatcc
      121 ggtggtggcg ggtctggagg aggaggtagt ggtggaggtg gctctggtac cggtggagct
      181 agacccgggg agcgcccctt ccagtgtcgc atttgcatgc ggaacttttc gactaatcaa
      241 aaattggaag ttcatacccg tactcatacc ggtgaaaaac cgtttcagtg tcggatctgt
      301 atgcgaaatt tctccgttag acataatttg caaagacatc tacgtacgca caccggcgag
      361 aagccattcc aatgccgaat atgcatgcgc aacttcagtc aacatccaaa tttgactaga
      421 cacctaaaaa cccacctgag aggatctggc tctggcccta agaagaagcg caaagttggc
      481 tctggcatgt acccatacga cgtaccagat tacgcttag
//
