LOCUS       sequence-313146-        9984 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of self-cleaving membrane targeting peptide 
            containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B
            cleavage site, msGFP and the NS3/4A protease. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Vevea JD, Chapman ER
  TITLE     Acute disruption of the synaptic vesicle membrane protein 
            synaptotagmin 1 using knockoff in mouse hippocampal neurons.
  JOURNAL   eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469.
  PUBMED    32515733
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-06-9"; volume: "9. pii: 56469. doi"; pages: " 
            10.7554/eLife.56469"
COMMENT     Sequence Label: pF(UG) hSyn NS34a-msGFP-'S-mito
FEATURES             Location/Qualifiers
     source          1..9984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      479..488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1199..1220)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1807..1828
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2075..2663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2128..2148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2546..2557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2666..2682)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2667..2683)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3188..3368
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3394..3411)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3400..3624
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3670..4098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3757..3776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3967..3988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4107..4127)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4112..4441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4292..4427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4354..4373
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4489..4536
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4555..4929
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5059..5180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5096..5115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5150..5169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5229..5245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5229..5245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5242..5264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5253..5269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5277..5283,5284..5301,5302..5307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5322..5343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5460..5477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5631..6219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5711..5730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6390..7181,7182..7250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7013..7032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7251..7355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7430..7449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7621..8000
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8002..8200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8155..8175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8218..8398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8445..8570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9063..9296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9481..9525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9823..9940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
       61 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
      121 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
      181 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
      241 ctgacgtcac tcgccggtcc cccgtaaact ccccttcccg gccaccttgg tcgcgtccgc
      301 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
      361 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
      421 ggagtcgtgt cgtgcctgag agcgcaggta cggctagcgg atccggtacc gcgggcccgc
      481 caccatgggc tgcgtggtga tcgtgggcag gatcgtgctg agcggcagcg gcaccagcgc
      541 gcccatcacg gcgtacgccc agcagacgag aggcctccta gggtgtataa tcaccagcct
      601 gactggccgg gacaaaaacc aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca
      661 aaccttcctg gcaacgtgca tcaatggggt atgctggaca gtctaccacg gggccggaac
      721 gaggaccatc gcatcaccca agggtcctgt catccagatg tataccaatg tggaccaaga
      781 ccttgtgggc tggcccgctc ctcaaggttc ccgctcattg acaccctgta cctgcggctc
      841 ctcggacctt tacctggtca cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga
      901 tagcaggggt agcctgcttt cgccccggcc catttcctac ttgaaaggct cctcgggggg
      961 tccgctgttg tgccccgcgg gacacgccgt gggcctattc agggccgcgg tgtgcacccg
     1021 tggagtggct aaagcggtgg actttatccc tgtggagaac ctagagacaa ccatgagatc
     1081 cccggtgttc acggacaact cctctccacc agcagggtca ggttcttcgg gcagttcggg
     1141 gtccagcgga tctagtatgg atagcactga gagcctgttc accggggtgg tgcccatcct
     1201 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg agggcgaggg
     1261 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1321 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1381 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1441 gcgcaccatc accttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga
     1501 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1561 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcaccgccga
     1621 caagcagaag aacggcatca aggccaactt caagatccgc cacaacgtgg aggacggcag
     1681 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1741 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     1801 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcggatccgg
     1861 aagttcgggg tccagcggtt cttcaggcag ttccgaggat gtggtctgct gttcaatgtc
     1921 ctacggcagt ggatctaagc gcggagttcc tgtagctgtg gtgctgctgc cagtgtttgc
     1981 ccttaccctg gtagcagttt gggccttcgt gagataccga aagcagctct gaggccgcac
     2041 tcctcagcta gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2101 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2161 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2221 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2281 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2341 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2401 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2461 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2521 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2581 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2641 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     2701 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     2761 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     2821 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2881 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2941 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     3001 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     3061 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     3121 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     3181 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3241 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3301 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3361 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3421 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3481 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3541 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3601 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3661 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3721 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3781 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3841 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3901 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3961 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4021 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4081 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4201 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4261 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4321 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4381 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4441 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     4501 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     4561 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     4621 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     4681 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     4741 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     4801 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     4861 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     4921 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4981 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5041 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5101 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5161 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7381 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7441 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7501 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7561 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7621 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7681 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7741 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7801 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7861 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7921 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7981 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     8041 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     8101 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     8161 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     8221 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8281 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8341 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8401 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8461 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8521 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8581 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8641 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8701 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8761 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8821 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8881 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8941 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9001 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9061 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9121 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9181 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9241 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9301 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9361 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9421 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     9481 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9541 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9601 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9661 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9721 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     9781 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     9841 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9901 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     9961 cagagatcca gtttggttaa ttaa
//
