LOCUS       sequence-313147-       11147 bp    DNA     circular SYN 15-APR-2025
DEFINITION  iGluSnFR glutamate sensor containing a Golgi export sequence and and
            an ER exit motif for improved trafficking to the plasma membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Vevea JD, Chapman ER
  TITLE     Acute disruption of the synaptic vesicle membrane protein 
            synaptotagmin 1 using knockoff in mouse hippocampal neurons.
  JOURNAL   eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469.
  PUBMED    32515733
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2020-06-9"; volume: "9. pii: 56469. doi"; pages: " 
            10.7554/eLife.56469"
COMMENT     Sequence Label: pF(UG) CamKII sf iGluSnFR
FEATURES             Location/Qualifiers
     source          1..11147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1289
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1305..1314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     1311..1373
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     primer_bind     2339..2360
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2463..2484)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2949..2978
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3228..3816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3281..3301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3699..3710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3819..3835)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3820..3836)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4341..4521
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4547..4564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4553..4777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4823..5251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4910..4929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5120..5141
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5260..5280)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5265..5594
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5445..5580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5507..5526
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5642..5689
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5708..6082
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6212..6333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6249..6268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6303..6322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6382..6398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6382..6398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6395..6417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6406..6422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6430..6436,6437..6454,6455..6460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6475..6496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6613..6630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6784..7372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6864..6883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7543..8334,8335..8403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8166..8185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8404..8508)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8583..8602)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8774..9153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9155..9353
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9308..9328
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9371..9551
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9598..9723
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10216..10449
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10634..10678
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10976..11093
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cattatggcc ttaggtcact tcatctccat ggggttcttc ttctgatttt ctagaaaatg
       61 agatgtgggt gcagagagct tcctcagtga cctgcccagg gtcacatcag aaatgtcaga
      121 gctagaactt gaactcagat tactaatctt aaattccatg ccttgggggc atgcaagtac
      181 gatatacaga aggagtgaac tcattagggc agatgaccaa tgagtttagg aaagaagagt
      241 ccagggcagg gtacatctac accacccgcc cagccctggg tgagtccagc cacgttcacc
      301 tcattatagt tgcctctctc cagtcctacc ttgacgggaa gcacaagcag aaactgggac
      361 aggagcccca ggagaccaaa tcttcatggt ccctctggga ggatgggtgg ggagagctgt
      421 ggcagaggcc tcaggagggg ccctgctgct cagtggtgac agataggggt gagaaagcag
      481 acagagtcat tccgtcagca ttctgggtct gtttggtact tcttctcacg ctaaggtggc
      541 ggtgtgatat gcacaatggc taaaaagcag ggagagctgg aaagaaacaa ggacagagac
      601 agaggccaag tcaaccagac caattcccag aggaagcaaa gaaaccatta cagagactac
      661 aagggggaag ggaaggagag atgaattagc ttcccctgta aaccttagaa cccagctgtt
      721 gccagggcaa cggggcaata cctgtctctt cagaggagat gaagttgcca gggtaactac
      781 atcctgtctt tctcaaggac catcccagaa tgtggcaccc actagccgtt accatagcaa
      841 ctgcctcttt gccccactta atcccatccc gtctgttaaa agggccctat agttggaggt
      901 gggggaggta ggaagagcga tgatcacttg tggactaagt ttgttcgcat ccccttctcc
      961 aaccccctca gtacatcacc ctgggggaac agggtccact tgctcctggg cccacacagt
     1021 cctgcagtat tgtgtatata aggccagggc aaagaggagc aggttttaaa gtgaaaggca
     1081 ggcaggtgtt ggggaggcag ttaccggggc aacgggaaca gggcgtttcg gaggtggttg
     1141 ccatggggac ctggatgctg acgaaggctc gcgaggctgt gagcagccac agtgccctgc
     1201 tcagaagccc caagctcgtc agtcaagccg gttctccgtt tgcactcagg agcacgggca
     1261 ggcgagtggc ccctagttct gggggcagcc ggtaccgcgg gcccgccacc atggagacag
     1321 acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt gacagatctg
     1381 ccgcaggcag cacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
     1441 aatcttcagt gcctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
     1501 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
     1561 taaaactgat tccgattacc gcacaaaacc gtattccact gctgcaaaac ggcactttcg
     1621 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
     1681 acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa gggtggcgat atcaaagatt
     1741 ttgccaacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
     1801 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg
     1861 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgacgtgc
     1921 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
     1981 cgcagtctca ggaggcctac ggttgtatgt tgcgtaaaga tgatccgcag ttcaaaaagc
     2041 tgatggatga caccatcgct caggtgcaga cctccggtga agcggaaaaa tggtttgata
     2101 agtggttcaa aaatccaatt ctggtgagcc acaacgtcta tatcaccgcc gacaagcaga
     2161 agaacggcat caaggcgaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc
     2221 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2281 accactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca
     2341 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     2401 agggcggtac cggagggagc atgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2461 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgcgc ggcgagggcg
     2521 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2581 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     2641 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2701 aggagcgcac catcagcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt
     2761 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2821 gcaacatcct ggggcacaag ctggagtaca actttaacaa ccccctgaac atgaatttcg
     2881 aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaaactgc
     2941 aggtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc caggacacgc
     3001 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
     3061 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcaagtac ctgcagaaaa
     3121 gaagggagcg gagacgccag gagaacgcaa acagcttttg ctatgaaaat gaagttgccc
     3181 tctaaggccg cactcctcag ctagaattcg atatcaagct tatcgataat caacctctgg
     3241 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3301 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3361 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3421 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3481 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3601 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3661 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3721 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3781 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     3841 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     3901 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     3961 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4021 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     4081 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     4141 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     4201 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     4261 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     4321 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4381 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4441 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4501 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4561 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4621 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4681 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4741 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4801 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4861 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4921 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4981 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5041 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5101 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5161 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5221 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5341 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5401 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5521 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5581 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     5641 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     5701 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     5761 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     5821 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     5881 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     5941 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     6001 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     6061 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     6121 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6181 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6241 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6301 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6361 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6421 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6481 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6541 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6601 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7561 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7621 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7681 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7741 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7801 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7861 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7921 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7981 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8041 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8101 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8161 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8221 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8281 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8341 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8401 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8461 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8521 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     8581 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     8641 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     8701 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     8761 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     8821 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8881 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8941 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     9001 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     9061 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     9121 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     9181 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     9241 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     9301 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     9361 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9421 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9481 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9541 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     9601 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9661 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9721 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9781 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9841 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9901 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9961 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    10021 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
    10081 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    10141 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    10201 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    10261 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    10321 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    10381 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    10441 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10501 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10561 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10621 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10681 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10741 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10801 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    10861 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10921 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
    10981 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    11041 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    11101 attacaggga cagcagagat ccagtttggt tagaggggtc ggcaatt
//
