LOCUS       sequence-313190-        9892 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF 
            (1541-2818).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, 
            Waybright T, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan 
            A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D
  TITLE     Biochemical and structural analyses reveal that the tumor suppressor
            neurofibromin (NF1) forms a high-affinity dimer.
  JOURNAL   J Biol Chem. 2020 Jan 24;295(4):1105-1119. doi: 
            10.1074/jbc.RA119.010934. Epub 2019 Dec 13.
  PUBMED    31836666
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010934"; journalName: "J Biol Chem"; date: 
            "2020-01-24- 24"; volume: "295"; issue: "4"; pages: "1105-1119"
COMMENT     Sequence Label: R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)
FEATURES             Location/Qualifiers
     source          1..9892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             167..1264
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1121..1130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1238..1261
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     protein_bind    1283..1307
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1307..1327
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(5166..5190)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5335..5469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5372..5391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5426..5445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5498..5663
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5847..6301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5934..5953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6143..6164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6327..6431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6432..6500,6501..7292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6650..6669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7463..8051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7952..7971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  8356..8580
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8647..9180)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(9369..9374,9375..9391,9392..9397))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(9760..9869)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcaccatgg gatcccatca ccatcaccat cacggcaaaa tcgaagaagg
      181 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      241 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      301 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      361 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      421 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      481 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
      541 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag
      601 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
      661 gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa
      721 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
      781 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
      841 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
      901 ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg
      961 tattaacgct gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct
     1021 gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     1081 gtcttacgag gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca
     1141 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     1201 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     1261 gactaattcg ggatcctcga tcacaagttt gtacaaaaaa gttggcgaaa acctgtactt
     1321 ccaaggcagc ctgaacctga ccagcagcaa gttcgaggaa ttcatgaccc ggcaccaggt
     1381 gcacgagaaa gaagagttta aagccctgaa aaccctgagc atcttctacc aggccggcac
     1441 ctccaaggcc ggcaacccca tcttctacta cgtggccaga aggttcaaga caggccagat
     1501 caacggcgac ctgctgatct accacgtgct gctgacactg aagccctact acgccaagcc
     1561 ctacgagatc gtggtggatc tgacccacac cggccccagc aaccggttca agaccgactt
     1621 cctgagcaag tggttcgtgg tgttccccgg cttcgcctac gataacgtga gcgccgtgta
     1681 catctacaac tgcaacagct gggtgcggga gtacaccaag taccacgagc gcctgctgac
     1741 cggcctgaag ggctccaagc ggctggtgtt catcgactgc cccggcaagc tggccgagca
     1801 catcgagcac gagcagcaga aactgcctgc cgccaccctg gccctggaag aggacctgaa
     1861 ggtgttccac aacgccctga agctggccca caaggataca aaggtgtcca tcaaagtggg
     1921 cagcaccgcc gtgcaggtga ccagcgccga gaggacaaag gtgctgggcc agagcgtgtt
     1981 cctgaacgac atctactacg cctccgagat cgaggaaatc tgcctggtgg atgagaacca
     2041 gttcaccctg actatcgcca accagggcac ccctctgacc ttcatgcacc aggaatgcga
     2101 ggccatcgtc cagtccatca tccacatccg gaccagatgg gagctgtccc agcccgacag
     2161 catcccccag cacaccaaga tccggcccaa ggatgtgccc ggcaccctgc tgaatatcgc
     2221 tctgctgaat ctgggcagca gcgacccttc tctgcggagc gccgcctaca atctgctgtg
     2281 cgccctgacc tgcaccttca acctgaagat cgagggccag ctgctggaaa ccagcggcct
     2341 gtgcatcccc gccaacaaca ccctgtttat cgtgagcatc agcaagaccc tggccgccaa
     2401 cgagcctcac ctgaccctgg aatttctgga agagtgcatc agcggcttca gcaagagcag
     2461 catcgagctg aagcacctgt gcctggaata catgaccccc tggctgtcta atctggtccg
     2521 gttctgcaag cacaacgacg acgccaagcg gcagagagtg accgccattc tggacaagct
     2581 gatcacaatg accatcaacg agaagcagat gtaccccagc atccaggcca agatctgggg
     2641 cagcctgggc cagatcaccg atctgctgga tgtggtgctg gacagcttca tcaagaccag
     2701 cgccaccggc ggactgggaa gcatcaaggc cgaggtgatg gccgatacag ccgtggccct
     2761 ggctagcggc aacgtgaagc tggtgtccag caaggtgatc ggccggatgt gcaagatcat
     2821 cgacaagacc tgcctgagcc ccacccctac cctggaacag cacctgatgt gggacgatat
     2881 cgccatcctg gcccggtaca tgctgatgct gtccttcaac aatagcctgg acgtggccgc
     2941 ccatctgccc tacctgttcc acgtggtgac ctttctggtg gccaccggcc ctctgagcct
     3001 gagagccagc acccacggcc tggtgattaa catcatccac tccctgtgca cctgctccca
     3061 gctgcacttc agcgaggaaa ccaagcaggt gctgagactg agcctgaccg agttctccct
     3121 gcccaagttc tacctgctgt ttggcatcag caaagtgaag tctgccgccg tgatcgcctt
     3181 caggtccagc tacagggaca gatctttctc ccccggaagc tacgagagag agacattcgc
     3241 cctgacaagc ctggaaaccg tgacagaggc cctgctggaa atcatggaag cctgcatgag
     3301 agacatcccc acctgcaagt ggctcgacca gtggaccgaa ctggcccaga ggttcgcctt
     3361 ccagtacaac cccagcctgc agcctagagc cctggtggtg ttcggctgca tctccaagag
     3421 agtgagccac ggccagatta agcagatcat caggatcctg tccaaggccc tggaaagctg
     3481 tctgaagggc cccgacacct acaactccca ggtgctgatc gaggccacag tgatcgccct
     3541 gaccaaactg cagccactgc tgaataagga cagccccctg cacaaagccc tgttctgggt
     3601 ggccgtggcc gtgctgcagc tggacgaggt gaacctgtac agcgccggga ccgctctgct
     3661 ggaacagaat ctgcacaccc tggactccct gagaatcttc aacgacaaga gccccgagga
     3721 agtgttcatg gccatccgga accccctgga atggcactgc aagcagatgg accacttcgt
     3781 gggcctgaac ttcaacagca acttcaactt cgccctggtc ggacacctgc tgaagggcta
     3841 cagacacccc agcccagcca tcgtggccag gaccgtgcgg atcctgcata ccctgctgac
     3901 cctggtgaac aagcaccgga actgcgacaa gtttgaggtg aacacccaga gcgtggccta
     3961 cctggctgcc ctgctgacag tgagcgagga agtgcggagc cggtgcagcc tgaagcacag
     4021 gaagagcctg ctcctcactg atatcagcat ggaaaacgtg cccatggaca cctaccccat
     4081 ccaccacggc gaccccagct acaggaccct gaaagaaacc cagccctggt cctcccctaa
     4141 gggcagcgag ggctatctgg ccgccaccta tcctaccgtc ggccagacaa gccctagggc
     4201 ccgcaagtcc atgagcctgg acatgggcca gcctagccag gccaacacca agaaactgct
     4261 gggcacccgg aagagcttcg accacctgat cagcgacacc aaggccccca agaggcagga
     4321 aatggaaagc ggcatcacca ccccccccaa gatgcggaga gtggccgaga cagactacga
     4381 gatggaaacc cagcggatca gcagctccca gcagcaccct cacctgcgga aagtgtccgt
     4441 gtccgagagc aatgtgctgc tggacgaaga ggtgctgacc gatcctaaga tccaggccct
     4501 gctgctgact gtcctggcca ccctggtgaa gtacaccacc gacgagttcg atcagcggat
     4561 cctgtacgag tacctggccg aggccagcgt ggtgtttccc aaggtgttcc ctgtggtgca
     4621 caatctgctg gatagcaaga tcaacaccct gctgtctctg tgccaggacc ccaacctgct
     4681 gaatcccatc cacggcatcg tgcagagtgt ggtgtaccac gaagagagcc cccctcagta
     4741 ccagaccagc tacctgcagt ccttcggctt caacggcctg tggcggtttg ccggcccttt
     4801 cagcaagcag acccagatcc ccgactacgc cgaactgatc gtgaagttcc tggacgccct
     4861 gatcgatacc tacctgcccg gcatcgacga ggaaacctcc gaggaatctc tgctgacccc
     4921 cacctcccct tatcctccag ccctgcagtc ccagctgtcc atcaccgcca acctgaacct
     4981 gtccaactcc atgaccagcc tggccacatc tcagcacagc cctggcatcg acaaagaaaa
     5041 cgtggagctg tctcccacca ccggccactg caactccggc aggaccagac acggaagcgc
     5101 cagccaggtg cagaagcaga gaagcgccgg cagcttcaag cggaacagca tcaagaaaat
     5161 cgtgtaatca actttcttgt acaaagtggt gatcgagcta gctagaagct tgtcgagaag
     5221 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     5281 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     5341 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5401 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5461 gtctggatct gatcactgct tgagcctagg agatccgaac cagataagtg aaatctagtt
     5521 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     5581 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     5641 cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa
     5701 catcctgcca actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa
     5761 tgaaaatttt tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct
     5821 tatttgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     5881 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5941 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6001 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6061 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6121 gttggagtcc acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6181 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6241 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6301 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6601 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6661 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6961 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7141 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7321 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7381 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7441 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7501 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7561 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7621 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7681 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7741 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7801 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7861 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7921 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7981 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8041 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8101 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8161 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8221 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8281 accgcataga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg
     8341 ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa
     8401 ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
     8461 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
     8521 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
     8581 gcggcgttgt gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac
     8641 gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc
     8701 caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac
     8761 ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc
     8821 ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg
     8881 ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc
     8941 agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg
     9001 atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg
     9061 catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc
     9121 acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat
     9181 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     9241 cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     9301 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     9361 tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt
     9421 tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct
     9481 ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc
     9541 tgttcttcta cggcaaggtg ctgtgcacgg atctgccctt gcttcaggag atcggtagac
     9601 ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg
     9661 gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt
     9721 ggctactcga gatcccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt
     9781 tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata
     9841 atatattgtg ttgggttgaa ttaaaggtcc gtatactccg gaatattaat ag
//
