LOCUS       sequence-31322-e         894 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX3 (3DM1)
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             291..308
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(361..379)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      375..422
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      459..886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(546..565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     756..777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcgaa tttgtcgtgg aaaaagtact agatcgacgt
      121 gtagtgaatg ggaaagtgga atatttcctg aagtggaagg gatttacaga tgctgacaat
      181 acttgggaac ctgaagaaaa tttagattgt ccagaattga ttgaagcgtt tcttaactct
      241 cagaaagctg gcaaagaaaa atgatgacga agcttgcggc cgcactcgag caccaccacc
      301 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      361 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      421 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      481 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      541 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      601 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      661 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      721 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      781 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      841 ctattggtta aaaatgagct gatttaacaa aaatttaacg cgaatttaca atta
//
