LOCUS       sequence-313323-         717 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Overexpresses SCN2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 717)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SCN2B_OE_1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(647..671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             673..714
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgcacagag atgcctggct acctcgccct gccttcagcc tcacggggct cagtctcttt
       61 ttctctttgg tgccaccagg acggagcatg gaggtcacag tacctgccac cctcaacgtc
      121 ctcaatggct ctgacgcccg cctgccctgc accttcaact cctgctacac agtgaaccac
      181 aaacagttct ccctgaactg gacttaccag gagtgcaaca actgctctga ggagatgttc
      241 ctccagttcc gcatgaagat cattaacctg aagctggagc ggtttcaaga ccgcgtggag
      301 ttctcaggga accccagcaa gtacgatgtg tcggtgatgc tgagaaacgt gcagccggag
      361 gatgagggga tttacaactg ctacatcatg aacccccctg accgccaccg tggccatggc
      421 aagatccatc tgcaggtcct catggaagag ccccctgagc gggactccac ggtggccgtg
      481 attgtgggtg cctccgtcgg gggcttcctg gctgtggtca tcttggtgct gatggtggtc
      541 aagtgtgtga ggagaaaaaa agagcagaag ctgagcacag atgacctgaa gaccgaggag
      601 gagggcaaga cggacggtga aggcaacccg gatgatggcg ccaagtaccc aactttcttg
      661 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtag
//
