LOCUS       sequence-313331-        1359 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Overexpresses KCNK12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1359)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNK12_OE_1
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1289..1313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1315..1356
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgtcctccc gcagcccccg gcccccgccc cgccgtagcc gccgccgcct gccgcgcccc
       61 tcctgctgct gctgctgctg ccgccgttcg cacctcaacg aggacaccgg ccgcttcgtg
      121 ctgctggcgg cgctcatcgg cctctacctg gtggcgggtg ccacagtctt ctcggcgctc
      181 gagagccccg gcgaggcgga ggcgcgggcg cgctggggcg ccacgctgcg caacttcagc
      241 gctgcgcacg gcgtggccga gccagagctg cgcgccttcc tccggcacta cgaggccgcg
      301 ctggccgccg gcgtccgcgc cgacgcgctg cgcccgcgct gggacttccc cggcgccttc
      361 tacttcgtgg gcaccgtggt gtcaaccata ggtttcggca tgaccacccc cgcgacggtg
      421 ggcgggaagg ccttcctcat cgcctacggg ctgttcggct gcgctgggac catcctgttc
      481 ttcaacctct tcctggagcg catcatctcg ctgctggcct tcatcatgcg cgcctgccgg
      541 gagcgccagc tgcgccgcag cggcctgctg cccgccacct tccgccgcgg ctccgcgctc
      601 tcggaggccg acagcctggc gggctggaag ccctcggtgt accacgtgct gctcatcctg
      661 ggcctgttcg ccgtgctgct gtcctgctgc gcctcggcca tgtacaccag cgtggagggc
      721 tgggactacg tggactcgct ctacttctgc ttcgtcacct tcagcaccat cggcttcggg
      781 gacctggtga gcagccagca cgccgcctac cggaaccagg ggctctaccg cctgggcaac
      841 ttcctcttca tcctgctcgg cgtgtgctgc atttactcgc tcttcaacgt catctccatc
      901 ctcatcaagc aggtgctcaa ctggatgctg cgcaagctga gctgccgctg ctgcgcgcgc
      961 tgctgcccgg ctcctggcgc gcccctggcc cggcgcaatg ccatcacccc aggctcccgg
     1021 ctgcgccgcc gcctggccgc gctcggcgcc gaccccgcgg cccgcgacag cgacgccgag
     1081 ggccgccgcc tctcgggcga gctcatctcc atgcgcgacc tcacggcctc caacaaggtg
     1141 tcgctggcgc tgctgcagaa gcagctgtcg gagacggcca acggctaccc gcgcagcgtg
     1201 tgcgtcaaca cgcgccagaa cggcttctcg ggcggcgtgg gcgcgctggg catcatgaac
     1261 aaccggctgg ccgagaccag cgcctccagg acagctttct tgtacaaagt ggttggtaag
     1321 cctatcccta accctctcct cggtctcgat tctacgtag
//
