LOCUS       sequence-313392-        1701 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Overexpresses KCNN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1701)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNN1_OE_1
FEATURES             Location/Qualifiers
     source          1..1701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1631..1655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1657..1698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgaacagcc acagctacaa tggcagcgtg gggcggccgc tgggcagcgg gccgggcgcc
       61 ctgggacgag accctccgga ccctgaggcc ggccaccccc cacaaccccc gcacagcccg
      121 ggcctccagg tggtagtggc caagagtgag ccagcccggc cctcacccgg cagcccccgg
      181 gggcagcccc aggaccagga cgatgacgag gatgatgagg aagatgaggc cggcaggcag
      241 agagcctcgg ggaaaccctc aaatgtgggc caccgcctgg gccaccggcg ggcgctcttc
      301 gagaagcgga agcgcctcag cgactatgcc ctcattttcg gcatgtttgg catcgtcgtc
      361 atggtgacgg agaccgagct gtcctggggg gtgtacacca aggagtctct gtactcattc
      421 gcactcaaat gcctcatcag cctctccacg gccatcctgc tgggtctcgt tgtcctctac
      481 catgcccggg agatccagct gttcatggtg gacaacgggg ctgatgactg gcgcatcgcc
      541 atgacctgcg agcgcgtgtt cctcatctcg ctagagctgg cagtgtgcgc cattcacccg
      601 gtgcccggcc actaccgctt cacgtggacg gcgcggctgg ccttcacgta cgcgccctcg
      661 gtggccgagg ccgacgtgga cgtgctgctg tccatcccca tgttcctgcg cctctacctg
      721 ctgggccggg tgatgctact gcacagcaaa atcttcacgg acgcctcgag ccgcagcatc
      781 ggggccctca acaagatcac cttcaacacg cgcttcgtca tgaagacact catgaccatc
      841 tgccccggca ccgtgctgct ggtcttcagc atctcctcct ggatcatcgc agcctggacc
      901 gtgcgcgtct gcgagaggta ccacgacaag caggaagtga ccagcaactt cctgggggcc
      961 atgtggctga tttccatcac cttcctctcc attggctacg gcgacatggt gccccacacc
     1021 tactgcggga agggtgtgtg cctgctcact ggcatcatgg gagctggctg taccgcgctc
     1081 gtggtggctg tggtggctcg gaagctggag ctcaccaagg ctgagaagca cgtgcacaac
     1141 ttcatgatgg acactcagct caccaagcgg gtaaaaaacg ccgctgctaa cgttctcagg
     1201 gagacgtggc tcatctacaa acataccagg ctggtgaaga agccagacca agcccgggtt
     1261 cggaaacacc agcgtaagtt cctccaagcc atccatcagg ctcagaagct ccggagtgtg
     1321 aagatcgagc aagggaagct gaacgaccag gctaacacgc ttaccgacct agccaagacc
     1381 cagaccgtca tgtacgacct tgtatcggag ctgcacgctc agcacgagga gctggaggcc
     1441 cgcctggcca ccctggaaag ccgcttggat gcgctgggtg cctctctaca ggccctgcct
     1501 ggcctcatcg cccaagccat acgcccaccc ccgcctcccc tgcctcccag gcccggcccc
     1561 ggcccccaag accaggcagc ccggagctcc ccctgccggt ggacgcccgt ggccccctcg
     1621 gactgcgggt tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc
     1681 ctcggtctcg attctacgta g
//
